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Species: Mus musculus
Genetic Insert: Cdk9 donor region including homology arms and HaloTag
Vector Backbone Description: Vector Backbone:pbluescript; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29889212
Proper citation: RRID:Addgene_113113 Copy
Species: Mus musculus
Genetic Insert: Gata3 donor region including homology arms and T2A-H2B-eGFP
Vector Backbone Description: Vector Backbone:pbluescript; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29889212
Proper citation: RRID:Addgene_113119 Copy
Species: Mus musculus
Genetic Insert: Dppa1 donor region including homology arms and T2A-H2B-Halo
Vector Backbone Description: Vector Backbone:pbluescript; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29889212
Proper citation: RRID:Addgene_113118 Copy
Species: Mus musculus
Genetic Insert: Fgfr2 donor region including homology arms and T2A-H2B-Halo
Vector Backbone Description: Vector Backbone:pbluescript; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29889212
Proper citation: RRID:Addgene_113117 Copy
Species: Mus musculus
Genetic Insert: pgk-1 5' and 3' regions
Vector Backbone Description: Backbone Size:2700; Vector Backbone:pUC18; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:3440520
Comments: Includes 500 bp mouse PGK promoter, 832 bp neo, 460 bp PGK 3' end.
Proper citation: RRID:Addgene_11333 Copy
Species: Mus musculus
Genetic Insert: mAID-BE3
Vector Backbone Description: Backbone Size:3399; Vector Backbone:CMV; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30125268
Proper citation: RRID:Addgene_113420 Copy
Species: Mus musculus
Genetic Insert: mA1-BE3
Vector Backbone Description: Backbone Size:3399; Vector Backbone:CMV; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30125268
Proper citation: RRID:Addgene_113421 Copy
Species: Mus musculus
Genetic Insert: p37
Vector Backbone Description: Vector Backbone:pGEX-6P-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30344098
Proper citation: RRID:Addgene_113501 Copy
Species: Mus musculus
Genetic Insert: p37
Vector Backbone Description: Vector Backbone:pGEX-6P-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30344098
Proper citation: RRID:Addgene_113504 Copy
Species: Mus musculus
Genetic Insert: p37
Vector Backbone Description: Vector Backbone:pGEX-6P-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30344098
Proper citation: RRID:Addgene_113503 Copy
Species: Mus musculus
Genetic Insert: p37
Vector Backbone Description: Vector Backbone:pGEX-6P-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30344098
Proper citation: RRID:Addgene_113502 Copy
Species: Mus musculus
Genetic Insert: PAR2
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29411438
Proper citation: RRID:Addgene_113609 Copy
Species: Mus musculus
Genetic Insert: I-Ab beta Derp1-117
Vector Backbone Description: Backbone Size:3818; Vector Backbone:pRMHa-3; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29956152
Comments: This construct, when co-expressed with the corresponding alpha chain construct in Drosophila S2 cells and induced with copper sulfate, results in secreted expression of soluble peptide:MHCII complexes. The antigenic peptide sequence (residues 117-127 of Der p 1 allergen) is encoded as a fusion to the N-terminus of I-Ab beta, connected via a Gly-Ser linker. This fusion is encoded just downstream of the signal peptide, which should be cleaved during post-translational processing. Alternative antigenic peptides can be cloned in place of Der p 1 117-127 by exploiting the XmaI and SpeI restriction sites flanking this sequence.
Proper citation: RRID:Addgene_113639 Copy
Species: Mus musculus
Genetic Insert: I-Ab alpha BirA
Vector Backbone Description: Backbone Size:3808; Vector Backbone:pRMHa-3; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29956152
Comments: This construct, when co-expressed with the corresponding beta chain construct in Drosophila S2 cells and induced with copper sulfate, results in secreted expression of soluble peptide:MHCII complexes.
Proper citation: RRID:Addgene_113638 Copy
Species: Mus musculus
Genetic Insert: Fndc3b
Vector Backbone Description: Vector Backbone:pcDNA3.1(+) Laccase2 MCS Exon; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31541092
Proper citation: RRID:Addgene_126444 Copy
Species: Mus musculus
Genetic Insert: m-Smoothened
Vector Backbone Description: Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26291458
Proper citation: RRID:Addgene_126415 Copy
Species: Mus musculus
Genetic Insert: m-Smoothened
Vector Backbone Description: Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26291458
Proper citation: RRID:Addgene_126406 Copy
Species: Mus musculus
Genetic Insert: 2XMCP-Ezh2
Vector Backbone Description: Vector Backbone:pHAGE-UbiC; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31053162
Proper citation: RRID:Addgene_126590 Copy
Species: Mus musculus
Genetic Insert: Ighg3
Vector Backbone Description: Backbone Size:5729; Vector Backbone:pUC19; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_127160 Copy
Species: Mus musculus
Genetic Insert: Ighg2c
Vector Backbone Description: Backbone Size:5729; Vector Backbone:pUC19; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_127159 Copy
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