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Species: Synthetic
Genetic Insert: G-CEPIA1er
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4819; Vector Backbone:pCMV/myc/ER; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24923787
Proper citation: RRID:Addgene_58215 Copy
Species: Synthetic
Genetic Insert: CEPIA2mt
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4799; Vector Backbone:pCMV/myc/mito; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24923787
Proper citation: RRID:Addgene_58218 Copy
Species: Escherichia coli
Genetic Insert: hygromycin B phosphotransferase
Vector Backbone Description: Vector Backbone:pFGL815; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Reference:
Sweigard J, Chumley F, Carroll A, Farrall L, Valent B (1997) A series of vectors for fungal transformation. Fungal Genet Newsl 44: 52-53
Proper citation: RRID:Addgene_58223 Copy
Species: Streptomyces sp.
Genetic Insert: phosphinothricin acetyl transferase
Vector Backbone Description: Vector Backbone:pFGL815; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Reference:
Sweigard J, Chumley F, Carroll A, Farrall L, Valent B (1997) A series of vectors for fungal transformation. Fungal Genet Newsl 44: 52-53
Proper citation: RRID:Addgene_58226 Copy
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: ECFP/Venus cytosolic calcium biosensor. Excitation = 433 / 515; Emission = 475 / 528
Proper citation: RRID:Addgene_58182 Copy
Vector Backbone Description: Vector Backbone:pSNAPf-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: With SNAP tag TBRII contains the H385N mutation .
Proper citation: RRID:Addgene_58187 Copy
Species: Synthetic
Genetic Insert: CEPIA4mt
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4799; Vector Backbone:pCMV/myc/mito; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24923787
Proper citation: RRID:Addgene_58220 Copy
Species: Magnaporthe grisea
Genetic Insert: ILV2
Vector Backbone Description: Vector Backbone:pFGL815; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Reference:
Sweigard J, Chumley F, Carroll A, Farrall L, Valent B (1997) A series of vectors for fungal transformation. Fungal Genet Newsl 44: 52-53
Please note that the actual sequence of the SUR gene in this plasmid differs from the Genbank reference sequence AF013601 by a G120V mutation in the ILV2 locus. G120V does not contribute to the sulfonyl urea resistance per se.
Proper citation: RRID:Addgene_58221 Copy
Species: Mus musculus
Genetic Insert: Plexin A1
Vector Backbone Description: Vector Backbone:pBS KS+; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22723296
Comments: cDNA from expressed sequence tag (EST) IMAGE clones (Invitrogen), clone ID 1853022 (Plxna1).
For antisense RNA transcription, use EcoR1 and T7.
Proper citation: RRID:Addgene_58237 Copy
Species: Homo sapiens
Genetic Insert: PDHA1
Vector Backbone Description: Vector Backbone:pSNAPf-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: With SNAP tag.
Proper citation: RRID:Addgene_58195 Copy
Species: GATEWAY DEST cassette
Genetic Insert: attR1-Cm(R)-CcdB-attR2
Vector Backbone Description: Backbone Marker:Adrian Trasher, UCL, London, UK; Vector Backbone:pHR-SIN-CSGW; Vector Types:Mammalian Expression, Lentiviral, RNAi, GATEWAY destionation vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24841113
Comments: There are some minor discrepancies between the depositor's assembled sequence and Addgene's quality control sequence. These differences should not affect the function of the plasmid.
Proper citation: RRID:Addgene_58245 Copy
Species: Homo sapiens
Genetic Insert: PITP alpha
Vector Backbone Description: Backbone Marker:EMD Millipore; Backbone Size:4671; Vector Backbone:pET14b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14962392
Proper citation: RRID:Addgene_58258 Copy
Genetic Insert: Enhanced Green Fluorescence Protein, Puromycin resistance gene
Vector Backbone Description: Backbone Size:4941; Vector Backbone:HSC1 retroviral vector; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23752310
Comments: Please note that there is a small deletion (32bp) just after the 3’LTR inside the cloning vector that removes the M13R primer region. The depositing lab was able to produce viruses with good titer and doesn’t think the missing 32bp is a major concern.
Proper citation: RRID:Addgene_58254 Copy
Species: Rattus norvegicus
Genetic Insert: Nrxn1beta AS4(-)
Vector Backbone Description: Backbone Size:4869; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17360903
Comments: See map & sequence file for insert-releasing enzymes (e.g. NheI / KpnI).
Insert can also be released by single digest using XhoI.
The rat neurexin 1 beta encoded by this plasmid lacks alternatively spliced segment 4 (AS4), and contains the region homologous to alternatively spliced segment 5 (AS5) of mouse neurexin 1 beta (compare Schreiner et al., Neuron, 2014 [DOI: 10.1016/j.neuron.2014.09.011]).
Note: HA-epitope tag was introduced at the mature N-terminus.
Proper citation: RRID:Addgene_58267 Copy
Species: Homo sapiens
Genetic Insert: FADD
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:8519; Vector Backbone:pLVX-IRES-Hygro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24581498
Proper citation: RRID:Addgene_58263 Copy
Species: Rattus norvegicus
Genetic Insert: Nrxn1alpha
Vector Backbone Description: Backbone Size:4869; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17360903
Comments: See map & sequence file for insert-releasing enzymes (e.g. NheI / XmaI).
Insert can also be released without HA-tag (e.g. by SpeI / XmaI digest).
The rat neurexin 1 alpha encoded by this plasmid lacks alternatively spliced segment 4 (AS4), and contains the regions homologous to alternatively spliced segment 3 and 5 (AS3 and AS5, respectively) of mouse neurexin 1 alpha (compare Schreiner et al., Neuron, 2014 [DOI: 10.1016/j.neuron.2014.09.011]).
Note: HA-epitope tag was introduced at the mature N-terminus.
Proper citation: RRID:Addgene_58266 Copy
Species: Homo sapiens
Genetic Insert: IL36RN
Vector Backbone Description: Backbone Marker:Origene; Backbone Size:4900; Vector Backbone:pCMV6-entry; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37414245
Proper citation: RRID:Addgene_58324 Copy
Species: S.pyogenes
Genetic Insert: SpCas9
Vector Backbone Description: Vector Backbone:pLKO.005; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24952903
Proper citation: RRID:Addgene_58329 Copy
Species: Homo sapiens
Genetic Insert: Mis12-FKBPx3
Vector Backbone Description: Vector Backbone:inducible knockdown/cDNA RMCE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24637323
Proper citation: RRID:Addgene_58285 Copy
Species: Homo sapiens
Genetic Insert: IL36RN
Vector Backbone Description: Backbone Marker:Origene; Backbone Size:4900; Vector Backbone:pCMV6-entry; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37414245
Proper citation: RRID:Addgene_58321 Copy
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