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Species: Rattus norvegicus
Genetic Insert: fRGECO2
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3684; Vector Backbone:pCI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30651353
Comments: fRGECO2 is the W44Y and D432A variant of the jRGECO1a gene cloned from the pGP-CMV vector (#61563) published by Dana et al. 2016.
Proper citation: RRID:Addgene_125244 Copy
Species: Rattus norvegicus
Genetic Insert: fRCaMP1
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3703; Vector Backbone:pCI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30651353
Comments: fRCaMP1 is the W44Y variant of the jRCaMP1a gene cloned from the pGP-CMV vector (#61562) published by Dana et al. 2016.
Proper citation: RRID:Addgene_125245 Copy
Species: Rattus norvegicus
Genetic Insert: fRGECO1
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3684; Vector Backbone:pCI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30651353
Comments: fRGECO1 is the D323A variant of the jRGECO1a gene cloned from the pGP-CMV vector (#61563) published by Dana et al. 2016.
Proper citation: RRID:Addgene_125243 Copy
Species: Rattus norvegicus
Genetic Insert: fRGECO2
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3955; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30651353
Comments: fRGECO2 is the W44Y, D432A variant of the jRGECO1a gene cloned from the pGP-CMV vector (#61563) published by Dana et al. 2016.
Proper citation: RRID:Addgene_125248 Copy
Species: Rattus norvegicus
Genetic Insert: fRGECO2
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3955; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30651353
Comments: fRGECO1 is the D323A variant of the jRGECO1a gene cloned from the pGP-CMV vector (#61563) published by Dana et al. 2016.
Proper citation: RRID:Addgene_125247 Copy
Species: Rattus norvegicus
Genetic Insert: fRCaMp1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3955; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30651353
Comments: fRCaMP1 is the W44Y variant of the jRCaMP1a gene cloned from the pGP-CMV vector (#61563) published by Dana et al. 2016.
Proper citation: RRID:Addgene_125249 Copy
Species: Rattus norvegicus
Genetic Insert: CCAAT/Enhancer-binding Protein beta LIP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5427; Vector Backbone:pcDNA3.1(-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: The C/EBPbeta gene encodes 3 in-frame translational isoforms (LAP*, LAP, and LIP). This plasmid is designed to express the LIP isoform of the protein.
For more information on the C/EBPbeta isoforms, please see the original reference:
Descombes, P., and Schibler, U. A liver-enriched transcriptional activator protein, LAP, and a transcriptional inhibitory protein, LIP, are translated from the same mRNA. Cell 67:569-579 (1991).
Proper citation: RRID:Addgene_12562 Copy
Species: Rattus norvegicus
Genetic Insert: calcium channel beta1b auxillary subunit
Vector Backbone Description: Backbone Marker:Genetics Institute, Inc, Cambridge, MA, USA; Backbone Size:5163; Vector Backbone:pMT2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9006976
Comments: Please note, although not a perfect match, the nucleotide sequence of this plasmid most closely matches NM_017346.1 Rattus norvegicus cacnb1 mRNA.
Proper citation: RRID:Addgene_107423 Copy
Species: Rattus norvegicus
Genetic Insert: cacnb4
Vector Backbone Description: Backbone Marker:Genetics Institute, Inc, Cambridge, MA, USA; Backbone Size:5163; Vector Backbone:pMT2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11756448
Comments: We received it in pBluescript and subcloned it into pMT2 plasmid.
Proper citation: RRID:Addgene_107426 Copy
Species: Rattus norvegicus
Genetic Insert: VAMP2
Vector Backbone Description: Backbone Size:3740; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29123102
Proper citation: RRID:Addgene_107369 Copy
Species: Rattus norvegicus
Genetic Insert: VGF8a
Vector Backbone Description: Vector Backbone:pUC; Vector Types:Cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:3839317
Comments: Note: The VGF insert contains a V480M mutation compared to the closest reference sequence. The depositor is unable to confirm what, if any, functional impact this has on the plasmid.
Proper citation: RRID:Addgene_107572 Copy
Species: Rattus norvegicus
Genetic Insert: VGF promoter
Vector Backbone Description: Vector Backbone:pUC; Vector Types:Cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:1570299
Proper citation: RRID:Addgene_107574 Copy
Species: Rattus norvegicus
Genetic Insert: synaptophysin-GCaMP6s, mRuby3
Vector Backbone Description: Backbone Marker:Bong-Kiun Kaang; Backbone Size:4476; Vector Backbone:pAAV-CW3SL-EGFP; Vector Types:Mammalian Expression, AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Comments: This plasmid contains a naturally-occurring a G→T mutation in one of the AAV ITRs at position 570bp. However, we have still obtained high titer AAV using this plasmid and robust in vivo expression in Cre+ mouse neurons.
Proper citation: RRID:Addgene_107737 Copy
Species: Rattus norvegicus
Genetic Insert: synaptophysin-GCaMP6s, mRuby3
Vector Backbone Description: Backbone Marker:Bong-Kiun Kaang; Backbone Size:3372; Vector Backbone:pAAV-CW3SL-EGFP; Vector Types:Mammalian Expression, AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_107736 Copy
Species: Rattus norvegicus
Genetic Insert: PKC zeta
Vector Backbone Description: Backbone Size:4650; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9768361
Proper citation: RRID:Addgene_10800 Copy
Species: Rattus norvegicus
Genetic Insert: PKC zeta
Vector Backbone Description: Backbone Size:4650; Vector Backbone:pCMV6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9768361
Comments: Constitutively active. Myr sequence from p60src. Note: There is a HindIII site in the insert ~1100bp from the 5'end.
Proper citation: RRID:Addgene_10802 Copy
Species: Rattus norvegicus
Genetic Insert: GABRD
Vector Backbone Description: Vector Backbone:pEGFP N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16181787
Proper citation: RRID:Addgene_119223 Copy
Species: Rattus norvegicus
Genetic Insert: GABRG1
Vector Backbone Description: Vector Backbone:pEGFP N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_119224 Copy
Species: Rattus norvegicus
Genetic Insert: Ywhae
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5514; Vector Backbone:pcDNA3.1 His C; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11864996
Proper citation: RRID:Addgene_11943 Copy
Species: Rattus norvegicus
Genetic Insert: GABRD/GABRG2
Vector Backbone Description: Vector Backbone:pCDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_119731 Copy
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