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Species: Homo sapiens
Genetic Insert: JNK1a1
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:5053; Vector Backbone:pGEX-4T-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23233447
Proper citation: RRID:Addgene_47574 Copy
Species: Synthetic
Genetic Insert: Codon-optimised P12p
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:6633; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24043421
Proper citation: RRID:Addgene_47726 Copy
Species: Synthetic
Genetic Insert: Codon-optimised P38
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:6633; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24043421
Proper citation: RRID:Addgene_47727 Copy
Species: Homo sapiens
Genetic Insert: CALM3
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4000; Vector Backbone:pGEMEX-2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9828009
Comments: We observed in preliminary experiments that IPTG added to the growth media did not induce substantial additional CLP expression. In addition, we found that the addition of glucose to the growth medium significantly inhibited CLP expression from this vector. An NdeI site at position 3259 has been abolished.
Proper citation: RRID:Addgene_47600 Copy
Species: Synthetic
Genetic Insert: Codon-optimised P12
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:6633; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24043421
Proper citation: RRID:Addgene_47725 Copy
Genetic Insert: TP-1 promoter
Vector Backbone Description: Backbone Marker:described in Kato, et al. 1997 PMID 9374409 ; Vector Backbone:pGA981-6; Vector Types:Notch reporter construct; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16508309
Comments: This plasmid was constructed from the pGa981-6 plasmid by replacing the luciferase gene in the original pGA981-6 plasmid with dsRedExpress (Clontech) using XhoI/SmaI sites.
The construction of pGA981-6 was described as: "Six copies of this oligonucleotide were cloned in the BamHI site:
5' strand GATCCCGACT CGTGGGAAAA TGGGCGGAAG GGCACCGTGG GAAAATAGTA............
3' strand ............GGCTGA GCACCCTTTT ACCCGCCTTC CCGTGGCACC CTTTTATCAT CTAG"
Please note that Addgene's sequencing results indicate that the first and fourth ERE-TP1 repeats differ from the above oligo sequence at the twelfth nucleotide (G->A). These differences may have occurred during construction of the original pGA981-6 plasmid.
Proper citation: RRID:Addgene_47683 Copy
Species: Synthetic
Genetic Insert: Codon-optimised MSP5
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:6633; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24043421
Proper citation: RRID:Addgene_47718 Copy
Species: Entercoccus faecalis
Genetic Insert: ndoE
Vector Backbone Description: Backbone Marker:GE Helthcare; Backbone Size:4974; Vector Backbone:pGEX-5X-3; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15028731
Proper citation: RRID:Addgene_47716 Copy
Species: Synthetic
Genetic Insert: Codon-optimised RAMA
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:6633; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24043421
Proper citation: RRID:Addgene_47786 Copy
Species: Synthetic
Genetic Insert: Codon-optimised TLP
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:6633; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24043421
Proper citation: RRID:Addgene_47787 Copy
Species: Synthetic
Genetic Insert: Codon-optimised PTEX150
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:6633; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24043421
Proper citation: RRID:Addgene_47788 Copy
Species: Synthetic
Genetic Insert: Codon-optimised SPATR
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:6633; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24043421
Proper citation: RRID:Addgene_47782 Copy
Species: Synthetic
Genetic Insert: Codon-optimised RhopH3
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:6633; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24043421
Proper citation: RRID:Addgene_47781 Copy
Species: Synthetic
Genetic Insert: Codon-optimised RH2b (partial)
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:6633; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24043421
Proper citation: RRID:Addgene_47778 Copy
Species: Arabidopsis thaliana
Genetic Insert: AmTrac-100
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pDR GW; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23840931
Proper citation: RRID:Addgene_47771 Copy
Species: Arabidopsis thaliana
Genetic Insert: AmTrac
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pDR GW; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23840931
Proper citation: RRID:Addgene_47770 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: MepTrac-H194E
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pDR GW; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23840931
Proper citation: RRID:Addgene_47764 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: meptrac
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pDR GW; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23840931
Proper citation: RRID:Addgene_47768 Copy
Species: Arabidopsis thaliana
Genetic Insert: AmTrac-GS
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pDR GW; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23840931
Proper citation: RRID:Addgene_47769 Copy
Genetic Insert: PesaR-luxCDABE-term
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:2934; Vector Backbone:pET-17b; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23879176
Comments: PesaR-luxCDABE-transcriptional terminator fragment from pCS-PesaRlux is between XhoI and BglII sites in pET17b.
PesaR (274bp, indicated as lowercase letters in the 2nd partial sequence for PesaR between XhoI and BamHI) is located between XhoI and BamHI sites. luxCDABE is located between two NotI sites. The transcriptional terminator is located downstream of luxCDABE between the second NotI and BglII.
Sequencing primers provided were used to sequence PesaR.
Proper citation: RRID:Addgene_47802 Copy
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