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Species: Danio rerio
Genetic Insert: Targeted 2aGal4VP16 protein trap; secondary marker gamma crystallin BFP
Vector Backbone Description: Backbone Marker:K.Clark; Backbone Size:7200; Vector Backbone:pKTol2-SE; Vector Types:Targetable insertional mutagen and reporter system; Bacterial Resistance:Kanamycin
Comments: This plasmid is designed to have homology domains cloned into the 5' end and 3' end. Bfuai is used to clone in the 5' homology domain, while BspQi is used to clone in the 3' homology domain.
Proper citation: RRID:Addgene_188015 Copy
Species: Danio rerio
Genetic Insert: Targeted 2aGal4VP16 protein trap; secondary marker gamma crystallin GFP
Vector Backbone Description: Backbone Marker:K.Clark; Backbone Size:7200; Vector Backbone:pKTol2-SE; Vector Types:Targetable insertional mutagen and reporter system; Bacterial Resistance:Kanamycin
Comments: This plasmid is designed to have homology domains cloned into the 5' end and 3' end. Bfuai is used to clone in the 5' homology domain, while BspQi is used to clone in the 3' homology domain.
Proper citation: RRID:Addgene_188017 Copy
Species: Danio rerio
Genetic Insert: Targeted 2aGal4VP16 protein trap; secondary marker cmlc2 BFP
Vector Backbone Description: Backbone Marker:K.Clark; Backbone Size:7200; Vector Backbone:pKTol2-SE; Vector Types:Targetable insertional mutagen and reporter system; Bacterial Resistance:Kanamycin
Comments: This plasmid is designed to have homology domains cloned into the 5' end and 3' end. Bfuai is used to clone in the 5' homology domain, while BspQi is used to clone in the 3' homology domain.
Proper citation: RRID:Addgene_188019 Copy
Species: Danio rerio
Genetic Insert: Targeted mRFP protein trap; secondary marker insulin GFP
Vector Backbone Description: Backbone Marker:K.Clark; Backbone Size:7200; Vector Backbone:pKTol2-SE; Vector Types:Targetable insertional mutagen and reporter system; Bacterial Resistance:Kanamycin
Comments: This plasmid is designed to have homology domains cloned into the 5' end and 3' end. Bfuai is used to clone in the 5' homology domain, while BspQi is used to clone in the 3' homology domain.
Proper citation: RRID:Addgene_188010 Copy
Species: Danio rerio
Genetic Insert: cytosolic acvr2b-CIB1N
Vector Backbone Description: Vector Backbone:pCS2; Vector Types:In vitro transcription; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40145591
Comments: Please visit https://doi.org/10.1101/2024.04.11.588875 for bioRxiv preprint.
Proper citation: RRID:Addgene_161720 Copy
Species: Danio rerio
Genetic Insert: dr-Ubi promoter
Vector Backbone Description: Vector Backbone:pDONRP4-P1r; Vector Types:Other, Gateway cloning; Bacterial Resistance:Kanamycin
Comments: Please visit https://doi.org/10.1101/2024.07.13.603267 for bioRxiv preprint.
Proper citation: RRID:Addgene_224542 Copy
Species: Danio rerio
Genetic Insert: drAgo2
Vector Backbone Description: Vector Backbone:pCS2+; Vector Types:Fish expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29272705
Proper citation: RRID:Addgene_221712 Copy
Species: Danio rerio
Genetic Insert: drAgo2
Vector Backbone Description: Vector Backbone:pCS2+; Vector Types:Fish expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29272705
Proper citation: RRID:Addgene_221711 Copy
Species: Danio rerio
Genetic Insert: drAgo2D-E
Vector Backbone Description: Vector Backbone:pCS2+; Vector Types:Fish expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29272705
Proper citation: RRID:Addgene_221714 Copy
Species: Danio rerio
Genetic Insert: drAgo2Y-F
Vector Backbone Description: Vector Backbone:pCS2+; Vector Types:Fish expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29272705
Proper citation: RRID:Addgene_221715 Copy
Species: Danio rerio
Genetic Insert: drAgo2Y-F
Vector Backbone Description: Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29272705
Proper citation: RRID:Addgene_221732 Copy
Species: Danio rerio
Genetic Insert: drAgo2D-E
Vector Backbone Description: Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29272705
Proper citation: RRID:Addgene_221731 Copy
Species: Danio rerio
Genetic Insert: drAgo2D-A
Vector Backbone Description: Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29272705
Proper citation: RRID:Addgene_221734 Copy
Species: Danio rerio
Genetic Insert: Zinc finger array targeting HACE1
Vector Backbone Description: Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18657511
Comments: This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene HACE1. Please note that this plasmid does NOT contain the HACE1 sequence.
Users must order the complementary plasmid HACE1_L (OZ565) [Addgene plasmid 33379] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene.
Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290/292 (Addgene plasmids 21872 & 21873), pMLM800/802 (Addgene plasmids 27202 & 27203).
The difference between pMLM800/802 and pMLM290/292 is the length of the “spacer” sequence in the full ZFN target site. If the spacer is 7 bp, scientists should use pMLM800/802. If the spacer is 5 or 6 bps, scientists should use pMLM290/292.
pMLM290/292 is identical to pST1374 except that it harbors two mutations in the FokI nuclease domain (Q486E, I499L; aka the “-“ mutation see Miller et al., Nat. Biotech 2007, PMID 17603475) which confers heterodimeric behavior on these domains
This zinc finger array was tested for binding activity to the sequence 5'-GCTGGAGAA-3' in a bacterial two hybrid assay, and resulted in 7.96 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus).
Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication.
Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4 (10):1471-1501. Pubmed ID: 19798082)
"Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934)
Proper citation: RRID:Addgene_33380 Copy
Species: Danio rerio
Genetic Insert: Zinc finger array targeting dio2
Vector Backbone Description: Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18657511
Comments: This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene dio2. Please note that this plasmid does NOT contain the dio2 sequence.
Users must order the complementary plasmid dio2_R (OZ570) [Addgene plasmid 33384] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene.
Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290/292 (Addgene plasmids 21872 & 21873), pMLM800/802 (Addgene plasmids 27202 & 27203).
The difference between pMLM800/802 and pMLM290/292 is the length of the “spacer” sequence in the full ZFN target site. If the spacer is 7 bp, scientists should use pMLM800/802. If the spacer is 5 or 6 bps, scientists should use pMLM290/292.
pMLM290/292 is identical to pST1374 except that it harbors two mutations in the FokI nuclease domain (Q486E, I499L; aka the “-“ mutation see Miller et al., Nat. Biotech 2007, PMID 17603475) which confers heterodimeric behavior on these domains
This zinc finger array was tested for binding activity to the sequence 5'-GGAGCAGCT-3' in a bacterial two hybrid assay, and resulted in 7.90 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus).
Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication.
Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082)
"Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934)
Proper citation: RRID:Addgene_33383 Copy
Species: Danio rerio
Genetic Insert: Zinc finger array targeting dio2
Vector Backbone Description: Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18657511
Comments: This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene dio2. Please note that this plasmid does NOT contain the dio2 sequence.
Users must order the complementary plasmid dio2_L (OZ569) [Addgene plasmid 33383] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene.
Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290/292 (Addgene plasmids 21872 & 21873), pMLM800/802 (Addgene plasmids 27202 & 27203).
The difference between pMLM800/802 and pMLM290/292 is the length of the “spacer” sequence in the full ZFN target site. If the spacer is 7 bp, scientists should use pMLM800/802. If the spacer is 5 or 6 bps, scientists should use pMLM290/292.
pMLM290/292 is identical to pST1374 except that it harbors two mutations in the FokI nuclease domain (Q486E, I499L; aka the “-“ mutation see Miller et al., Nat. Biotech 2007, PMID 17603475) which confers heterodimeric behavior on these domains
This zinc finger array was tested for binding activity to the sequence 5'-TAAGGTGGT-3' in a bacterial two hybrid assay, and resulted in 8.35 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus).
Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication.
Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082)
"Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934)
Proper citation: RRID:Addgene_33384 Copy
Species: Danio rerio
Genetic Insert: Zinc finger array targeting atp2c1
Vector Backbone Description: Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18657511
Comments: This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene atp2c1. Please note that this plasmid does NOT contain the atp2c1 sequence.
Users must order the complementary plasmid atp2c1_R (OZ568) [Addgene plasmid 33382] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene.
Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290/292 (Addgene plasmids 21872 & 21873), pMLM800/802 (Addgene plasmids 27202 & 27203).
The difference between pMLM800/802 and pMLM290/292 is the length of the “spacer” sequence in the full ZFN target site. If the spacer is 7 bp, scientists should use pMLM800/802. If the spacer is 5 or 6 bps, scientists should use pMLM290/292.
pMLM290/292 is identical to pST1374 except that it harbors two mutations in the FokI nuclease domain (Q486E, I499L; aka the “-“ mutation see Miller et al., Nat. Biotech 2007, PMID 17603475) which confers heterodimeric behavior on these domains
This zinc finger array was tested for binding activity to the sequence 5'-GTGGAGGTA-3' in a bacterial two hybrid assay, and resulted in 4.15 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus).
Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication.
Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082)
"Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934)
Proper citation: RRID:Addgene_33381 Copy
Species: Danio rerio
Genetic Insert: Zinc finger array targeting atp2c1
Vector Backbone Description: Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18657511
Comments: This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene atp2c1. Please note that this plasmid does NOT contain the atp2c1 sequence.
Users must order the complementary plasmid atp2c1_L (OZ567) [Addgene plasmid 33381] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene.
Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290/292 (Addgene plasmids 21872 & 21873), pMLM800/802 (Addgene plasmids 27202 & 27203).
The difference between pMLM800/802 and pMLM290/292 is the length of the “spacer” sequence in the full ZFN target site. If the spacer is 7 bp, scientists should use pMLM800/802. If the spacer is 5 or 6 bps, scientists should use pMLM290/292.
pMLM290/292 is identical to pST1374 except that it harbors two mutations in the FokI nuclease domain (Q486E, I499L; aka the “-“ mutation see Miller et al., Nat. Biotech 2007, PMID 17603475) which confers heterodimeric behavior on these domains
This zinc finger array was tested for binding activity to the sequence 5'-GCTGATGGA-3' in a bacterial two hybrid assay, and resulted in 14.13 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus).
Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication.
Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082)
"Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934)
Proper citation: RRID:Addgene_33382 Copy
Species: Danio rerio
Genetic Insert: Zinc finger array targeting esrrga
Vector Backbone Description: Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18657511
Comments: This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene esrrga. Please note that this plasmid does NOT contain the esrrga sequence.
Users must order the complementary plasmid esrrga_R (OZ564) [Addgene plasmid 33378] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene.
Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290/292 (Addgene plasmids 21872 & 21873), pMLM800/802 (Addgene plasmids 27202 & 27203).
The difference between pMLM800/802 and pMLM290/292 is the length of the “spacer” sequence in the full ZFN target site. If the spacer is 7 bp, scientists should use pMLM800/802. If the spacer is 5 or 6 bps, scientists should use pMLM290/292.
pMLM290/292 is identical to pST1374 except that it harbors two mutations in the FokI nuclease domain (Q486E, I499L; aka the “-“ mutation see Miller et al., Nat. Biotech 2007, PMID 17603475) which confers heterodimeric behavior on these domains
This zinc finger array was tested for binding activity to the sequence 5'-GCGGTAGCT-3' in a bacterial two hybrid assay, and resulted in 4.39 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus).
Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication.
Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082)
"Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934)
Proper citation: RRID:Addgene_33377 Copy
Species: Danio rerio
Genetic Insert: Zinc finger array targeting esrrga
Vector Backbone Description: Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18657511
Comments: This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene esrrga. Please note that this plasmid does NOT contain the esrrga sequence.
Users must order the complementary plasmid esrrga_L (OZ563) [Addgene plasmid 33377] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene.
Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290/292 (Addgene plasmids 21872 & 21873), pMLM800/802 (Addgene plasmids 27202 & 27203).
The difference between pMLM800/802 and pMLM290/292 is the length of the “spacer” sequence in the full ZFN target site. If the spacer is 7 bp, scientists should use pMLM800/802. If the spacer is 5 or 6 bps, scientists should use pMLM290/292.
pMLM290/292 is identical to pST1374 except that it harbors two mutations in the FokI nuclease domain (Q486E, I499L; aka the “-“ mutation see Miller et al., Nat. Biotech 2007, PMID 17603475) which confers heterodimeric behavior on these domains
This zinc finger array was tested for binding activity to the sequence 5'-TCTGAGGAG-3' in a bacterial two hybrid assay, and resulted in 5.42 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus).
Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication.
Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082)
"Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934)
Proper citation: RRID:Addgene_33378 Copy
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