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Species: Synthetic
Genetic Insert: 3xFlag-nsp5CS[VRLQ]-nsp10
Vector Backbone Description: Vector Backbone:pET11a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34198326
Comments: We have included the predicted cleavage site for nsp5 found between nsp9 and nsp10 (VRLQ) between the 3xFlag tag and nsp10 in this construct that should allow a purified nsp5 protease to cleave-out the 3xFlag tag similarly to what might happen in cells.
Proper citation: RRID:Addgene_169157 Copy
Species: Synthetic
Genetic Insert: XimD and PcPT
Vector Backbone Description: Vector Backbone:pSE152; Vector Types:Synthetic Biology; Bacterial Resistance:Apramycin
Defining Citation: PMID:32786357
Proper citation: RRID:Addgene_169109 Copy
Species: Synthetic
Genetic Insert: protein G - NanoLuc
Vector Backbone Description: Backbone Size:5369; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31909607
Proper citation: RRID:Addgene_169105 Copy
Species: Synthetic
Genetic Insert: protein G - mNeonGreen - NanoLuc
Vector Backbone Description: Backbone Size:5369; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31909607
Proper citation: RRID:Addgene_169106 Copy
Species: Synthetic
Genetic Insert: nsp7
Vector Backbone Description: Vector Backbone:pBIG1b (biGBac); Vector Types:Insect Expression; Bacterial Resistance:Ampicillin and Spectinomycin
Defining Citation: PMID:34198323
Comments: Baculovirus generation using Tn7 transposition (Bac-to-Bac).
Proper citation: RRID:Addgene_169178 Copy
Species: Synthetic
Genetic Insert: nsp8
Vector Backbone Description: Vector Backbone:pBIG1c (biGBac); Vector Types:Insect Expression; Bacterial Resistance:Ampicillin and Spectinomycin
Defining Citation: PMID:34198323
Comments: Baculovirus generation using Tn7 transposition (Bac-to-Bac).
Proper citation: RRID:Addgene_169179 Copy
Species: Synthetic
Genetic Insert: nsp7-Linker-nsp8
Vector Backbone Description: Vector Backbone:pBIG1b (biGBac); Vector Types:Insect Expression; Bacterial Resistance:Ampicillin and Spectinomycin
Defining Citation: PMID:34198323
Comments: Baculovirus generation using Tn7 transposition (Bac-to-Bac).
Proper citation: RRID:Addgene_169181 Copy
Species: Synthetic
Genetic Insert: 6xHis mCherry F30-Broccoli
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2665; Vector Backbone:pEXP5-NT; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35057626
Comments: Plasmid used for monitoring transcription and translation dynamics. Broccoli aptamer provides a fluorescent signal with DFHBI for mRNA level and mCherry provides fluorescent signal for protein level. Design and sequence of the Broccoli aptamer is from Filonov et al. (2015, doi: 10.1016/j.chembiol.2015.04.018).
Proper citation: RRID:Addgene_169234 Copy
Species: Synthetic
Genetic Insert: 3xFlag-His6-PLpro
Vector Backbone Description: Vector Backbone:pLIB (biGBac); Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34198325
Comments: Baculovirus generation using Tn7 transposition (Bac-to-Bac).
Proper citation: RRID:Addgene_169194 Copy
Species: Synthetic
Genetic Insert: GST-PLproCS-MBP
Vector Backbone Description: Vector Backbone:pGEX-4T-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34198325
Proper citation: RRID:Addgene_169195 Copy
Species: Synthetic
Genetic Insert: Luciferase
Vector Backbone Description: Vector Backbone:SIN40C; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34570885
Proper citation: RRID:Addgene_169307 Copy
Species: Synthetic
Genetic Insert: Luciferase
Vector Backbone Description: Vector Backbone:SIN40C; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34570885
Proper citation: RRID:Addgene_169308 Copy
Species: Synthetic
Genetic Insert: Luciferase
Vector Backbone Description: Vector Backbone:SIN40C; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34570885
Proper citation: RRID:Addgene_169317 Copy
Species: Synthetic
Genetic Insert: Double SapI flanked mNeongreen-P2A-Puro
Vector Backbone Description: Backbone Size:1969; Vector Backbone:Minimalistic Amp + ColE1 backbone; Vector Types:Targeting vector backbone; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35089911
Proper citation: RRID:Addgene_169228 Copy
Species: Synthetic
Genetic Insert: Double SapI flanked mTurquoise2-P2A-Puro
Vector Backbone Description: Backbone Size:1969; Vector Backbone:Minimalistic Amp + ColE1 backbone; Vector Types:Targeting vector backbone; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35089911
Proper citation: RRID:Addgene_169224 Copy
Species: Synthetic
Genetic Insert: Double SapI flanked mTurquoise2-P2A-Hygro
Vector Backbone Description: Backbone Size:1969; Vector Backbone:Minimalistic Amp + ColE1 backbone; Vector Types:Targeting vector backbone; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35089911
Proper citation: RRID:Addgene_169225 Copy
Species: Synthetic
Genetic Insert: Double SapI flanked mNeongreen
Vector Backbone Description: Backbone Size:1969; Vector Backbone:Minimalistic Amp + ColE1 backbone; Vector Types:Targeting vector backbone; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35089911
Proper citation: RRID:Addgene_169226 Copy
Species: Synthetic
Genetic Insert: 14His-SUMO-nsp12
Vector Backbone Description: Vector Backbone:pK27Sumo; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34198323
Proper citation: RRID:Addgene_169188 Copy
Species: Synthetic
Genetic Insert: Double SapI flanked mScarlet-P2A-Blast
Vector Backbone Description: Backbone Size:1969; Vector Backbone:Minimalistic Amp + ColE1 backbone; Vector Types:Targeting vector backbone; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35089911
Proper citation: RRID:Addgene_169221 Copy
Species: Synthetic
Genetic Insert: 3xFlag-His6-nsp13
Vector Backbone Description: Vector Backbone:pLIB (biGBac); Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34198322
Comments: Baculovirus generation using Tn7 transposition (Bac-to-Bac).
Proper citation: RRID:Addgene_169189 Copy
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