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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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LHP938 - Gal1-HA-Ub Resource Report Resource Website 1+ mentions |
RRID:Addgene_32175 | synthetic yeast Ubiquitin | Saccharomyces cerevisiae | Ampicillin | 2 µm S. cerevisiae origin. Orientation of insert determined by sequencing. | Backbone Marker:Invitrogen; Backbone Size:5800; Vector Backbone:pYES2; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:13:39 | 1 | ||
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LHP599 - pACT2-Ub Resource Report Resource Website |
RRID:Addgene_32170 | synthetic yeast Ubiquitin | Saccharomyces cerevisiae | Ampicillin | Backbone Marker:Clontech - Discontinued; Backbone Size:8100; Vector Backbone:pACT2 AD; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:13:39 | 0 | |||
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LHP905 - Gal1-Ub Resource Report Resource Website |
RRID:Addgene_32173 | synthetic yeast Ubiquitin | Saccharomyces cerevisiae | Ampicillin | CEN4 ARS1 S. cerevisiae replication. Ubiquitin was amplified using oligos which introduce BamHI sites on either end and cloned in to the BamHI site on the vector. Insertion was confirmed by digests and then orientation confirmed by sequencing. | Vector Backbone:N/A; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:13:39 | 0 | ||
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LHP424-YCplac33-STE2-WT Resource Report Resource Website 1+ mentions |
RRID:Addgene_32171 | STE2 | Saccharomyces cerevisiae | Ampicillin | Wildtype STE2 ORF including 1100bp upstream of the start codon (from SalI site) and 200bp downstream of the stop codon (HindIII). The wildtype 1.6kB HindIII fragment encoding STE2 was subcloned from LHP123 into the HindIII site of YCplac33. The upstream promoter sequence was obtained by dropping out the 1260bp EcoRI (upstream)/PstI fragment and ligating in the 2kB EcoRI/PstI fragment from pJR3. | Vector Backbone:YCPlac33; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:13:39 | 1 | ||
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LHP428-YCplac33-STE2-378Stop Resource Report Resource Website |
RRID:Addgene_32172 | STE2 | Saccharomyces cerevisiae | Ampicillin | STE2 ORF with a stop codon introduced at aa378. The 1.6kB STE2 378Stop -encoding HindIII fragment was subcloned from LHP147 into the HindIII site of YCplac33. The upstream promoter sequence was obtained by dropping out the 1260bp EcoRI(upstream)/PstI fragment and ligating in the 2KB EcoRI/PstI fragment from pJR3. | Vector Backbone:YCPlac33; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | Stop codon introduced at aa 378 | 2026-08-15 01:13:43 | 0 | |
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LHP809 - Ub-K63A-CYC1 Resource Report Resource Website |
RRID:Addgene_32166 | synthetic yeast Ubiquitin | Saccharomyces cerevisiae | Ampicillin | A ~1040 bp BamHI / HindIII fragment containing the CUP1 promoter, the synthetic yeast Ub gene and a CYC1 terminator sequence was ligated into the BamHI and HindIII sites of YEplac195. The resulting plasmid is approximately 6.281kb). K63A mutation was introduced by site-directed mutagenesis of LHP585 (Addgene 32168). 2 µm S. cerevisiae replication. | Vector Backbone:YEplac195; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | K63A | 2026-08-15 01:13:43 | 0 | |
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LHP398 - Ub-K48R/K63R Resource Report Resource Website |
RRID:Addgene_32167 | synthetic yeast Ubiquitin | Saccharomyces cerevisiae | Ampicillin | BglII-KpnI K48R/K63R fragment of synthetic yeast Ubiquitin cloned into YEp96 (D. Ecker, U PENN). 2 µm S. cerevisiae replication. | Vector Backbone:N/A; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | K48R and K63R | 2026-08-15 01:13:39 | 0 | |
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LHP585 - Ub-CYC1 Resource Report Resource Website |
RRID:Addgene_32168 | uba1 yeast | Saccharomyces cerevisiae | Ampicillin | PMID:12628920 | A ~1040 bp BamHI / HindIII fragment containing the CUP1 promoter, the synthetic yeast Ub gene and a CYC1 terminator sequence was ligated into the BamHI and HindIII sites of YEplac195. The resulting plasmid is approximately 6.281kb). 2 µm S. cerevisiae replication. | Vector Backbone:YEplac195; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:13:39 | 0 | |
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LPH2781 - Ub-K63R Resource Report Resource Website |
RRID:Addgene_32160 | synthetic yeast Ubiquitin | Saccharomyces cerevisiae | Ampicillin | BamHI-KpnI fragment from YEp96 (D. Ecker, U PENN) was cloned into pES7 (Mike Ellison, University of Alberta) cut with BamHI/KpnI. K63R mutation introduced by quickchange of LHP308 (Addgene plasmid 32151). 2 µm S. cerevisiae origin | Backbone Marker:Mike Ellison - University of Alberta; Vector Backbone:pES7; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | K63R | 2026-08-15 01:13:39 | 0 | |
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LHP416-PAN1-HA Resource Report Resource Website |
RRID:Addgene_32159 | PAN1 | Saccharomyces cerevisiae | Ampicillin | PAN1-HA including 1kB upstream sequence and 280bp downstream sequence of PAN1 ORF. | Vector Backbone:Unknown; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:13:43 | 0 | ||
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LHP334-pcDNA3-STE2 Resource Report Resource Website |
RRID:Addgene_32158 | STE2 | Saccharomyces cerevisiae | Ampicillin | Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:13:39 | 0 | |||
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LHP1472 - EDE1 Resource Report Resource Website |
RRID:Addgene_32238 | EDE1 | Saccharomyces cerevisiae | Ampicillin | Vector Backbone:YCplac33; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:13:39 | 0 | |||
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LHP1468 - VPS23 Resource Report Resource Website |
RRID:Addgene_32239 | VPS23 | Saccharomyces cerevisiae | Ampicillin | HA tag is likely 3xHA and likely C-terminal | Backbone Marker:ATCC; Vector Backbone:pRS316; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:13:44 | 0 | ||
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LHP1048 - pFB-RSP5 Resource Report Resource Website |
RRID:Addgene_32434 | RSP5 | Saccharomyces cerevisiae | Ampicillin | Backbone Marker:Invitrogen; Vector Backbone:pFastbac; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:13:41 | 0 | |||
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LHP1463 - INP52 Resource Report Resource Website |
RRID:Addgene_32432 | INP52 | Saccharomyces cerevisiae | Ampicillin | PMID:14761940 | INP52 was amplified from yeast genomic DNA using 5′ PstI and 3′ BamHI site-containing oligonucleotides, digested and ligated into PstI- and BamHI-digested YCplac22. The NotI site was inserted at the 5′-end of INP52 via QuikChange™ mutagenesis. A triple HA epitope was ligated into the NotI site to generate pHA-INP52 (LHP1463). | Backbone Marker:ATCC; Vector Backbone:YCplac22; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:13:41 | 0 | |
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LHP1049 - pFB-YCK2 Resource Report Resource Website |
RRID:Addgene_32433 | YCK2 | Saccharomyces cerevisiae | Ampicillin | Backbone Marker:Invitrogen; Vector Backbone:pFastbac; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin | Y193L | 2026-08-15 01:13:46 | 0 | ||
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pENTR L1-20XUAS-R5 Resource Report Resource Website |
RRID:Addgene_32302 | 20XUAS | Saccharomyces cerevisiae | Kanamycin | PMID:21931740 | Please see http://www.everyvector.com/sequences/show_public/12859 for additional plasmid map. | Backbone Marker:Invitrogen; Backbone Size:2609; Vector Backbone:pDONR 221 P1-P5r; Vector Types:Gateway MultiSite entry vector; Bacterial Resistance:Kanamycin | 2026-08-15 01:13:40 | 0 | |
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pENTR R4-GAL4-R3 Resource Report Resource Website |
RRID:Addgene_32309 | GAL4 | Saccharomyces cerevisiae | Kanamycin | PMID:21931740 | Please see http://www.everyvector.com/sequences/show_public/12862 for additional plasmid map. | Backbone Marker:Invitrogen; Backbone Size:2667; Vector Backbone:pDONR 221 P4r-P3r; Vector Types:Gateway MultiSite entry clone; Bacterial Resistance:Kanamycin | 2026-08-15 01:13:40 | 0 | |
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LHP1021 - BUL1 Resource Report Resource Website |
RRID:Addgene_32439 | BUL1 | Saccharomyces cerevisiae | Ampicillin | Backbone Marker:ATCC; Vector Backbone:YCplac111; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:13:46 | 0 | |||
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pTH375-SUP45-P174Q Resource Report Resource Website 1+ mentions |
RRID:Addgene_29383 | SUP45 gene encoding translation release factor 1 from S. cerevisiae | Saccharomyces cerevisiae | Ampicillin | PMID:20444877 | Backbone Size:5976; Vector Backbone:pRS315; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | P174Q mutant gene including genomic sequence 1432 bp upstream of AUG and 241 bp downstream of stop codon. | 2026-08-15 01:13:18 | 1 |
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