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  • Organism:saccharomyces cerevisiae (facet)


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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
LHP938 - Gal1-HA-Ub
 
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1+ mentions
RRID:Addgene_32175 synthetic yeast Ubiquitin Saccharomyces cerevisiae Ampicillin 2 µm S. cerevisiae origin. Orientation of insert determined by sequencing. Backbone Marker:Invitrogen; Backbone Size:5800; Vector Backbone:pYES2; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:13:39 1
LHP599 - pACT2-Ub
 
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RRID:Addgene_32170 synthetic yeast Ubiquitin Saccharomyces cerevisiae Ampicillin Backbone Marker:Clontech - Discontinued; Backbone Size:8100; Vector Backbone:pACT2 AD; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:13:39 0
LHP905 - Gal1-Ub
 
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RRID:Addgene_32173 synthetic yeast Ubiquitin Saccharomyces cerevisiae Ampicillin CEN4 ARS1 S. cerevisiae replication. Ubiquitin was amplified using oligos which introduce BamHI sites on either end and cloned in to the BamHI site on the vector. Insertion was confirmed by digests and then orientation confirmed by sequencing. Vector Backbone:N/A; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:13:39 0
LHP424-YCplac33-STE2-WT
 
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RRID:Addgene_32171 STE2 Saccharomyces cerevisiae Ampicillin Wildtype STE2 ORF including 1100bp upstream of the start codon (from SalI site) and 200bp downstream of the stop codon (HindIII). The wildtype 1.6kB HindIII fragment encoding STE2 was subcloned from LHP123 into the HindIII site of YCplac33. The upstream promoter sequence was obtained by dropping out the 1260bp EcoRI (upstream)/PstI fragment and ligating in the 2kB EcoRI/PstI fragment from pJR3. Vector Backbone:YCPlac33; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:13:39 1
LHP428-YCplac33-STE2-378Stop
 
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RRID:Addgene_32172 STE2 Saccharomyces cerevisiae Ampicillin STE2 ORF with a stop codon introduced at aa378. The 1.6kB STE2 378Stop -encoding HindIII fragment was subcloned from LHP147 into the HindIII site of YCplac33. The upstream promoter sequence was obtained by dropping out the 1260bp EcoRI(upstream)/PstI fragment and ligating in the 2KB EcoRI/PstI fragment from pJR3. Vector Backbone:YCPlac33; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin Stop codon introduced at aa 378 2026-08-15 01:13:43 0
LHP809 - Ub-K63A-CYC1
 
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RRID:Addgene_32166 synthetic yeast Ubiquitin Saccharomyces cerevisiae Ampicillin A ~1040 bp BamHI / HindIII fragment containing the CUP1 promoter, the synthetic yeast Ub gene and a CYC1 terminator sequence was ligated into the BamHI and HindIII sites of YEplac195. The resulting plasmid is approximately 6.281kb). K63A mutation was introduced by site-directed mutagenesis of LHP585 (Addgene 32168). 2 µm S. cerevisiae replication. Vector Backbone:YEplac195; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin K63A 2026-08-15 01:13:43 0
LHP398 - Ub-K48R/K63R
 
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RRID:Addgene_32167 synthetic yeast Ubiquitin Saccharomyces cerevisiae Ampicillin BglII-KpnI K48R/K63R fragment of synthetic yeast Ubiquitin cloned into YEp96 (D. Ecker, U PENN). 2 µm S. cerevisiae replication. Vector Backbone:N/A; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin K48R and K63R 2026-08-15 01:13:39 0
LHP585 - Ub-CYC1
 
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RRID:Addgene_32168 uba1 yeast Saccharomyces cerevisiae Ampicillin PMID:12628920 A ~1040 bp BamHI / HindIII fragment containing the CUP1 promoter, the synthetic yeast Ub gene and a CYC1 terminator sequence was ligated into the BamHI and HindIII sites of YEplac195. The resulting plasmid is approximately 6.281kb). 2 µm S. cerevisiae replication. Vector Backbone:YEplac195; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:13:39 0
LPH2781 - Ub-K63R
 
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RRID:Addgene_32160 synthetic yeast Ubiquitin Saccharomyces cerevisiae Ampicillin BamHI-KpnI fragment from YEp96 (D. Ecker, U PENN) was cloned into pES7 (Mike Ellison, University of Alberta) cut with BamHI/KpnI. K63R mutation introduced by quickchange of LHP308 (Addgene plasmid 32151). 2 µm S. cerevisiae origin Backbone Marker:Mike Ellison - University of Alberta; Vector Backbone:pES7; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin K63R 2026-08-15 01:13:39 0
LHP416-PAN1-HA
 
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RRID:Addgene_32159 PAN1 Saccharomyces cerevisiae Ampicillin PAN1-HA including 1kB upstream sequence and 280bp downstream sequence of PAN1 ORF. Vector Backbone:Unknown; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:13:43 0
LHP334-pcDNA3-STE2
 
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RRID:Addgene_32158 STE2 Saccharomyces cerevisiae Ampicillin Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:13:39 0
LHP1472 - EDE1
 
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RRID:Addgene_32238 EDE1 Saccharomyces cerevisiae Ampicillin Vector Backbone:YCplac33; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:13:39 0
LHP1468 - VPS23
 
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RRID:Addgene_32239 VPS23 Saccharomyces cerevisiae Ampicillin HA tag is likely 3xHA and likely C-terminal Backbone Marker:ATCC; Vector Backbone:pRS316; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:13:44 0
LHP1048 - pFB-RSP5
 
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RRID:Addgene_32434 RSP5 Saccharomyces cerevisiae Ampicillin Backbone Marker:Invitrogen; Vector Backbone:pFastbac; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:13:41 0
LHP1463 - INP52
 
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RRID:Addgene_32432 INP52 Saccharomyces cerevisiae Ampicillin PMID:14761940 INP52 was amplified from yeast genomic DNA using 5′ PstI and 3′ BamHI site-containing oligonucleotides, digested and ligated into PstI- and BamHI-digested YCplac22. The NotI site was inserted at the 5′-end of INP52 via QuikChange™ mutagenesis. A triple HA epitope was ligated into the NotI site to generate pHA-INP52 (LHP1463). Backbone Marker:ATCC; Vector Backbone:YCplac22; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:13:41 0
LHP1049 - pFB-YCK2
 
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RRID:Addgene_32433 YCK2 Saccharomyces cerevisiae Ampicillin Backbone Marker:Invitrogen; Vector Backbone:pFastbac; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin Y193L 2026-08-15 01:13:46 0
pENTR L1-20XUAS-R5
 
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RRID:Addgene_32302 20XUAS Saccharomyces cerevisiae Kanamycin PMID:21931740 Please see http://www.everyvector.com/sequences/show_public/12859 for additional plasmid map. Backbone Marker:Invitrogen; Backbone Size:2609; Vector Backbone:pDONR 221 P1-P5r; Vector Types:Gateway MultiSite entry vector; Bacterial Resistance:Kanamycin 2026-08-15 01:13:40 0
pENTR R4-GAL4-R3
 
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RRID:Addgene_32309 GAL4 Saccharomyces cerevisiae Kanamycin PMID:21931740 Please see http://www.everyvector.com/sequences/show_public/12862 for additional plasmid map. Backbone Marker:Invitrogen; Backbone Size:2667; Vector Backbone:pDONR 221 P4r-P3r; Vector Types:Gateway MultiSite entry clone; Bacterial Resistance:Kanamycin 2026-08-15 01:13:40 0
LHP1021 - BUL1
 
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RRID:Addgene_32439 BUL1 Saccharomyces cerevisiae Ampicillin Backbone Marker:ATCC; Vector Backbone:YCplac111; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:13:46 0
pTH375-SUP45-P174Q
 
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1+ mentions
RRID:Addgene_29383 SUP45 gene encoding translation release factor 1 from S. cerevisiae Saccharomyces cerevisiae Ampicillin PMID:20444877 Backbone Size:5976; Vector Backbone:pRS315; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin P174Q mutant gene including genomic sequence 1432 bp upstream of AUG and 241 bp downstream of stop codon. 2026-08-15 01:13:18 1

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