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pET11a_3xFlag-nsp10 (SARS-CoV-2)
 
Resource Report
Resource Website 		RRID:Addgene_169157 3xFlag-nsp5CS[VRLQ]-nsp10 Synthetic Ampicillin PMID:34198326 We have included the predicted cleavage site for nsp5 found between nsp9 and nsp10 (VRLQ) between the 3xFlag tag and nsp10 in this construct that should allow a purified nsp5 protease to cleave-out the 3xFlag tag similarly to what might happen in cells. Vector Backbone:pET11a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin Codon optimised for E. coli 2026-08-15 01:09:57 0
pIB1884-ximD-PCPT
 
Resource Report
Resource Website 		RRID:Addgene_169109 XimD and PcPT Synthetic Apramycin PMID:32786357 Vector Backbone:pSE152; Vector Types:Synthetic Biology; Bacterial Resistance:Apramycin 2026-08-15 01:09:57 0
pET28a Gx-NL
 
Resource Report
Resource Website 		RRID:Addgene_169105 protein G - NanoLuc Synthetic Kanamycin PMID:31909607 Backbone Size:5369; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin Ala 24 stop 2026-08-15 01:09:57 0
pET28a Gx-mNG-NL
 
Resource Report
Resource Website 		RRID:Addgene_169106 protein G - mNeonGreen - NanoLuc Synthetic Kanamycin PMID:31909607 Backbone Size:5369; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin Ala 24 stop 2026-08-15 01:09:57 0
pBIG1b_nsp7 (SARS-CoV-2)
 
Resource Report
Resource Website 		RRID:Addgene_169178 nsp7 Synthetic Ampicillin and Spectinomycin PMID:34198323 Baculovirus generation using Tn7 transposition (Bac-to-Bac). Vector Backbone:pBIG1b (biGBac); Vector Types:Insect Expression; Bacterial Resistance:Ampicillin and Spectinomycin Codon optimised for insect cells (Spodoptera frugiperda) 2026-08-15 01:09:57 0
pBIG1c_nsp8 (SARS-CoV-2)
 
Resource Report
Resource Website 		RRID:Addgene_169179 nsp8 Synthetic Ampicillin and Spectinomycin PMID:34198323 Baculovirus generation using Tn7 transposition (Bac-to-Bac). Vector Backbone:pBIG1c (biGBac); Vector Types:Insect Expression; Bacterial Resistance:Ampicillin and Spectinomycin Codon optimised for insect cells (Spodoptera frugiperda) 2026-08-15 01:09:58 0
pBIG1b_nsp7-Linker-nsp8 (SARS-CoV-2)
 
Resource Report
Resource Website 		RRID:Addgene_169181 nsp7-Linker-nsp8 Synthetic Ampicillin and Spectinomycin PMID:34198323 Baculovirus generation using Tn7 transposition (Bac-to-Bac). Vector Backbone:pBIG1b (biGBac); Vector Types:Insect Expression; Bacterial Resistance:Ampicillin and Spectinomycin Codon optimised for insect cells (Spodoptera frugiperda) 2026-08-15 01:09:57 0
pEXP5-NT/6xHis mCherry F30 Broccoli
 
Resource Report
Resource Website 		RRID:Addgene_169234 6xHis mCherry F30-Broccoli Synthetic Ampicillin PMID:35057626 Plasmid used for monitoring transcription and translation dynamics. Broccoli aptamer provides a fluorescent signal with DFHBI for mRNA level and mCherry provides fluorescent signal for protein level. Design and sequence of the Broccoli aptamer is from Filonov et al. (2015, doi: 10.1016/j.chembiol.2015.04.018). Backbone Marker:Invitrogen; Backbone Size:2665; Vector Backbone:pEXP5-NT; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:09:58 0
pLIB_3xFlag-His-PLpro (nsp3) (SARS-CoV-2)
 
Resource Report
Resource Website 		RRID:Addgene_169194 3xFlag-His6-PLpro Synthetic Ampicillin PMID:34198325 Baculovirus generation using Tn7 transposition (Bac-to-Bac). Vector Backbone:pLIB (biGBac); Vector Types:Insect Expression; Bacterial Resistance:Ampicillin Codon optimised for insect cells (Spodoptera frugiperda) 2026-08-15 01:09:58 0
pGEX4T1_GST-PLproCS-MBP
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_169195 GST-PLproCS-MBP Synthetic Ampicillin PMID:34198325 Vector Backbone:pGEX-4T-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:09:57 2
SIN40C.SFFV.Luciferase.IRES.dTomato
 
Resource Report
Resource Website 		RRID:Addgene_169307 Luciferase Synthetic Ampicillin PMID:34570885 Vector Backbone:SIN40C; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin 2026-08-15 01:09:58 0
SIN40C.SFFV.Luciferase.IRES.GFP
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_169308 Luciferase Synthetic Ampicillin PMID:34570885 Vector Backbone:SIN40C; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin 2026-08-15 01:09:58 2
SIN40C.TRE.Luciferase.IRES.dTomato.PGK.sfGFP.P2A.Tet3G
 
Resource Report
Resource Website 		RRID:Addgene_169317 Luciferase Synthetic Ampicillin PMID:34570885 Vector Backbone:SIN40C; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin 2026-08-15 01:09:59 0
TVBB C-term-mNeongreen-Puro
 
Resource Report
Resource Website 		RRID:Addgene_169228 Double SapI flanked mNeongreen-P2A-Puro Synthetic Ampicillin PMID:35089911 Backbone Size:1969; Vector Backbone:Minimalistic Amp + ColE1 backbone; Vector Types:Targeting vector backbone; Bacterial Resistance:Ampicillin 2026-08-15 01:09:58 0
TVBB C-term-mTurquoise2-Puro
 
Resource Report
Resource Website 		RRID:Addgene_169224 Double SapI flanked mTurquoise2-P2A-Puro Synthetic Ampicillin PMID:35089911 Backbone Size:1969; Vector Backbone:Minimalistic Amp + ColE1 backbone; Vector Types:Targeting vector backbone; Bacterial Resistance:Ampicillin 2026-08-15 01:09:58 0
TVBB C-term-mTurquoise2-Hygro
 
Resource Report
Resource Website 		RRID:Addgene_169225 Double SapI flanked mTurquoise2-P2A-Hygro Synthetic Ampicillin PMID:35089911 Backbone Size:1969; Vector Backbone:Minimalistic Amp + ColE1 backbone; Vector Types:Targeting vector backbone; Bacterial Resistance:Ampicillin 2026-08-15 01:09:58 0
TVBB N-term-mNeongreen
 
Resource Report
Resource Website 		RRID:Addgene_169226 Double SapI flanked mNeongreen Synthetic Ampicillin PMID:35089911 Backbone Size:1969; Vector Backbone:Minimalistic Amp + ColE1 backbone; Vector Types:Targeting vector backbone; Bacterial Resistance:Ampicillin 2026-08-15 01:09:58 0
pK27Sumo_His-SUMO-nsp12 (SARS-CoV-2)
 
Resource Report
Resource Website 		RRID:Addgene_169188 14His-SUMO-nsp12 Synthetic Kanamycin PMID:34198323 Vector Backbone:pK27Sumo; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin Codon optimised for E. coli 2026-08-15 01:09:58 0
TVBB C-term-mScarlet-Blast
 
Resource Report
Resource Website 		RRID:Addgene_169221 Double SapI flanked mScarlet-P2A-Blast Synthetic Ampicillin PMID:35089911 Backbone Size:1969; Vector Backbone:Minimalistic Amp + ColE1 backbone; Vector Types:Targeting vector backbone; Bacterial Resistance:Ampicillin 2026-08-15 01:09:58 0
pLIB_3xFlag-His6-nsp13 (SARS-CoV-2)
 
Resource Report
Resource Website 		RRID:Addgene_169189 3xFlag-His6-nsp13 Synthetic Ampicillin PMID:34198322 Baculovirus generation using Tn7 transposition (Bac-to-Bac). Vector Backbone:pLIB (biGBac); Vector Types:Insect Expression; Bacterial Resistance:Ampicillin Codon optimised for insect cells (Spodoptera frugiperda) 2026-08-15 01:09:57 0

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