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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pDL278 Resource Report Resource Website |
RRID:Addgene_46882 | Spectinomycin | PMID:1409970 | This plasmid may contain unstable elements. Prolonged growth in E. coli selects for deletions, but not in E. faecalis. Test multiple clones upon to ensure the full plasmid is selected. | Backbone Marker:Addgene plasmid 46881; Backbone Size:6733; Vector Backbone:pDL276; Vector Types:Bacterial Expression, Other, E. coli/Gram-positive Bacterial Shuttle Vector; Bacterial Resistance:Spectinomycin | 2026-08-15 01:15:36 | 0 | |||
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pMSP3535 Resource Report Resource Website 1+ mentions |
RRID:Addgene_46886 | Erythromycin | PMID:10964628 | This plasmid utilizes the NIsin Controlled Expression (NICE) system. NICE® is a registered trademark of NIZO food research BV, P.O.Box 20, 6710 BA Ede, The Netherlands. pMSP3535 contains the Nisin-inducible PnisA promoter, and the pAMB1 replicon for expression in gram-positive bacteria. For protein expression in E. faecalis, induce with 10-25 ng/mL nisin (Sigma, N-5764). See associated article for instructions on how to make nisin solution. Note: Nucleotide sequence encoding the signal sequence and one-third of the mature NisA (nisin) peptide remains associated with the PnisA promoter. The depositing laboratory recommends adding of a Stop codon to the 5' end of inserted sequences to avoid unintentional gene fusions. Addgene's quality control sequencing finds discrepancies with the depositor's provided sequencew which includes a mutation in RepE- F229L. This however is thought to not affect plasmid function. | Backbone Size:8353; Vector Backbone:pMSP3535; Vector Types:Bacterial Expression, Nisin-inducible expression; Gram-positive Bacterial Shuttle Vector; Bacterial Resistance:Erythromycin | 2026-08-15 01:15:36 | 6 | |||
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pMSP3535VA Resource Report Resource Website 1+ mentions |
RRID:Addgene_46887 | Kanamycin | PMID:10964628 | This plasmid utilizes the NIsin Controlled Expression (NICE) system. NICE® is a registered trademark of NIZO food research BV, P.O.Box 20, 6710 BA Ede, The Netherlands. pMSP3535VA contains the Nisin-inducible PnisA promoter, and the pVA380-1 replicon for expression in gram-positive bacteria. For protein expression in E. faecalis, induce with 10-25 ng/mL nisin (Sigma, N-5764). See associated article for instructions on how to make nisin solution. Note: Nucleotide sequence encoding the signal sequence and one-third of the mature NisA (nisin) peptide remains associated with the PnisA promoter. The depositing laboratory recommends adding of a Stop codon to the 5' end of inserted sequences to avoid unintentional gene fusions. Note: The theoretical full sequence uploaded here is NOT fully accurate - the NheI site at position 4414 has been lost, along with approximately 500bp of sequence in this region (compare the depositor's uploaded maps). The actual size of this plasmid is 8278 as listed here. The depositing lab has not resequenced the region in question, but the discrepancy does not affect plasmid function. Note: This plasmid has been shown to accumulate deletions when replicated in E. coli (or gram-negative bacteria). Screening transformants by restriction digest is recommended. Addgene's quality control sequencing finds discrepancies with the depositor's provided sequence, but they are not thought to affect plasmid function. | Backbone Size:8278; Vector Backbone:pMSP3535VA; Vector Types:Bacterial Expression, Nisin-inducible expression; Gram-positive Bacterial Shuttle Vector; Bacterial Resistance:Kanamycin | 2026-08-15 01:15:36 | 1 | |||
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pTDH3-dCas9 Resource Report Resource Website 1+ mentions |
RRID:Addgene_46920 | dCas9 | Ampicillin | PMID:23849981 | The Cas9 contains a N612K mutation that does not effect the function of the enzyme. | Backbone Size:6900; Vector Backbone:pJED103; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin | 2026-08-15 01:15:36 | 7 | ||
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pMA3174 Resource Report Resource Website |
RRID:Addgene_46880 | Ampicillin | PMID:22637478 | Backbone Marker:Homemade; Backbone Size:6990; Vector Backbone:pMA3211; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:15:36 | 0 | ||||
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pU6-sgGFP-NT1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_46914 | sgGFP-NT1 | Ampicillin | PMID:23849981 | gRNA target sequence GAATAGCTCAGAGGCCGAGG | Backbone Marker:Addgene; Backbone Size:8200; Vector Backbone:pSICO derivative; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin | 2026-08-15 01:15:36 | 8 | ||
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pU6-sgGAL4-1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_46915 | sgGAL4-1 | Ampicillin | PMID:23849981 | gRNA target sequence GTTGGAGCACTGTCCTCCGAACGT | Backbone Marker:Addgene; Backbone Size:8200; Vector Backbone:pSICO derivative; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin | 2026-08-15 01:15:36 | 2 | ||
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pMA3211 Resource Report Resource Website 1+ mentions |
RRID:Addgene_46879 | Ampicillin | PMID:22637478 | Backbone Marker:Homemade; Backbone Size:6642; Vector Backbone:pMA3211; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:15:36 | 1 | ||||
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pMSCV-LTR-dCas9-p65AD-BFP Resource Report Resource Website 1+ mentions |
RRID:Addgene_46913 | dCas9-p65AD-BFP fusion | Homo sapiens | Ampicillin | PMID:23849981 | Backbone Marker:Clontech; Backbone Size:6300; Vector Backbone:MSCV-puro; Vector Types:Mammalian Expression, Retroviral, CRISPR; Bacterial Resistance:Ampicillin | 2026-08-15 01:15:37 | 2 | ||
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pU6-sgCD71-2 Resource Report Resource Website |
RRID:Addgene_46918 | sgCD71 -2 | Homo sapiens | Ampicillin | PMID:23849981 | gRNA target sequence GGACGCGCTAGTGTGAGTGC | Backbone Marker:Addgene; Backbone Size:8200; Vector Backbone:pSICO derivative; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin | 2026-08-15 01:15:36 | 0 | |
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pU6-sgGAL4-4 Resource Report Resource Website 1+ mentions |
RRID:Addgene_46916 | sgGAL4-4 | Ampicillin | PMID:23849981 | gRNA target sequence GAACGACTAGTTAGGCGTGTA | Backbone Marker:Addgene; Backbone Size:8200; Vector Backbone:pSICO derivative; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin | 2026-08-15 01:15:37 | 1 | ||
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Beclin-HA S-A Resource Report Resource Website 1+ mentions |
RRID:Addgene_46994 | becn1 | Mus musculus | Ampicillin | PMID:23685627 | Vector Backbone:pqcxih; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | S14A | 2026-08-15 01:15:37 | 1 | |
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pMA3379 Resource Report Resource Website |
RRID:Addgene_46874 | soluble guanylate cyclase 1alpha3 | Rattus norvegicus | Ampicillin | PMID:22637478 | Please note that though there are some differences between Addgene's quality control sequence and the depositor's assembled sequence, this plasmid should function as reported. | Backbone Marker:Homemade; Backbone Size:6642; Vector Backbone:pMA3211; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:15:36 | 0 | |
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Beclin-HA S-D Resource Report Resource Website 1+ mentions |
RRID:Addgene_46995 | becn1 | Mus musculus | Ampicillin | PMID:23685627 | Vector Backbone:pqcxih; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | S14D | 2026-08-15 01:15:37 | 1 | |
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CS2-Myc-dLIS1 Resource Report Resource Website |
RRID:Addgene_47047 | Lis1 | Drosophila melanogaster | Ampicillin | PMID:23097494 | Vector Backbone:CS2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:15:37 | 0 | ||
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pMA3411 Resource Report Resource Website |
RRID:Addgene_46877 | soluble guanylate cyclase1 beta 3 | Rattus norvegicus | Ampicillin | PMID:22637478 | Backbone Size:5684; Vector Backbone:pMA1662; Vector Types:Retroviral; Bacterial Resistance:Ampicillin | E473K, C541D | 2026-08-15 01:15:36 | 0 | |
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CS2-HA-dASUN Resource Report Resource Website |
RRID:Addgene_47041 | Asunder | Drosophila melanogaster | Ampicillin | PMID:23097494 | Vector Backbone:CS2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:15:37 | 0 | ||
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CS2-CHY-dLIS1 Resource Report Resource Website |
RRID:Addgene_47045 | Lis1 | Drosophila melanogaster | Ampicillin | PMID:23097494 | Vector Backbone:CS2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:15:37 | 0 | ||
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CS2-Myc-mASUN Resource Report Resource Website |
RRID:Addgene_47044 | Asunder | Mus musculus | Ampicillin | PMID:23097494 | Vector Backbone:CS2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:15:38 | 0 | ||
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pRK5-EGFP-Tau E14 P301L Resource Report Resource Website 1+ mentions |
RRID:Addgene_46909 | Tau E14 P301L | Homo sapiens | Ampicillin | PMID:21172610 | WT htau construct (Addgene plasmid 46904) was used as a template to make mutations and contains human four-repeat tau lacking the N-terminal sequences (4R0N) and contains exons 1, 4 and 5, 7, and 9–13, intron 13, and exon 14. | Backbone Marker:Clontech; Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | P301L AND all 14 S/P or T/P amino acid residues (T111, T153, T175, T181, S199, S202, T205, T212, T217, T231, S235, S396, S404, and S422; numbering based on the longest 441-amino acid brain isoform of htau) changed to glutamate | 2026-08-15 01:15:36 | 2 |
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