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Species: Plasmodium falciparum
Genetic Insert: Pf-GMPS
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:7737; Vector Backbone:pET15-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: PDB: 3UOW. http://www.thesgc.org/structures/3UOW/
Proper citation: RRID:Addgene_36899 Copy
Species: Homo sapiens
Genetic Insert: DMTF1
Vector Backbone Description: Backbone Marker:SGC (Addgene plasmid 26096); Backbone Size:7325; Vector Backbone:pET28-MHL (Addgene plasmid 26096); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: PDB: 2LLK. http://www.thesgc.org/structures/2LLK/
Proper citation: RRID:Addgene_36895 Copy
Species: Homo sapiens
Genetic Insert: TNRC6A Delta 5a
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pCl-neo_NHA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21131274
Proper citation: RRID:Addgene_36936 Copy
Genetic Insert: T (i.e., ATG, start codon as a first exon)
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript SK(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22479386
Comments: The first exon (T) that contains the start codon is out of frame with the second GFP exon.
Proper citation: RRID:Addgene_36887 Copy
Species: P. aeruginosa
Genetic Insert: PaBphP-PCD
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5369; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22002602
Proper citation: RRID:Addgene_36920 Copy
Genetic Insert: GFP
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript SK(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22479386
Proper citation: RRID:Addgene_36888 Copy
Genetic Insert: red fluorescent protein
Vector Backbone Description: Backbone Size:4802; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18183494
Comments: This vector contains a MCS and combines the Cre/loxP with a bicistronic transcript that contains dual fluorescent and bioluminescent cell markers. mRFP was used as a marker for easy genotyping and tissue labeling. A chimeric membrane-localizing luciferase/eGFP fusion protein was designed as a secondary marker, which upon Cre activation, highlights the cells expressing the transgene with eGFP as well as luciferase activity.
Proper citation: RRID:Addgene_36921 Copy
Genetic Insert: T (i.e., ATG, start codon as a first exon)
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript SK(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22479386
Comments: ATG (T) and GFP are not in frame.
Proper citation: RRID:Addgene_36889 Copy
Species: Homo sapiens
Genetic Insert: Ago2
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pCl-neo_NHA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21131274
Proper citation: RRID:Addgene_36922 Copy
Species: Homo sapiens
Genetic Insert: TNGW1
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pCl-neo_NHA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21131274
Proper citation: RRID:Addgene_36923 Copy
Genetic Insert: tTA2
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript SK(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22479386
Proper citation: RRID:Addgene_36880 Copy
Species: Homo sapiens
Genetic Insert: TNGW1
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pCl-neo_NHA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21131274
Proper citation: RRID:Addgene_36928 Copy
Species: Homo sapiens
Genetic Insert: TNGW1
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pCl-neo_NHA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21131274
Comments: There is a typo in the NAR paper that incorrectly lists the N-term truncation as AA655 (instead of the correct AA566).
Proper citation: RRID:Addgene_36925 Copy
Genetic Insert: GFP
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript SK(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22479386
Proper citation: RRID:Addgene_36876 Copy
Genetic Insert: GFP
Vector Backbone Description: Backbone Size:8774; Vector Backbone:pROSA26-PA (Addgene plasmid Plasmid 21271); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22479386
Proper citation: RRID:Addgene_36878 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ScURA3
Vector Backbone Description: Vector Backbone:L22; Vector Types:Mammalian Expression, Bacterial Expression, Yeast Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22327349
Proper citation: RRID:Addgene_36911 Copy
Genetic Insert: tTA2
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript SK(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22479386
Proper citation: RRID:Addgene_36879 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ScURA3
Vector Backbone Description: Vector Backbone:L22-EGFP; Vector Types:Mammalian Expression, Bacterial Expression, Yeast Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22327349
Proper citation: RRID:Addgene_36912 Copy
Species: mycoplasma
Genetic Insert: myco
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3956; Vector Backbone:pCR4; Vector Types:; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_36872 Copy
Genetic Insert: tdTomato-3Myc
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript SK(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22479386
Proper citation: RRID:Addgene_36873 Copy
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