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Species: Homo sapiens
Genetic Insert: PYL-HDAC5
Vector Backbone Description: Backbone Size:5391; Vector Backbone:pAAV-CMV-GFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34494094
Proper citation: RRID:Addgene_114395 Copy
Species: Ramazzottius varieornatus, Tardigrade
Genetic Insert: mEGFP flanked by 1kbp upstream and downstream of the cahs3 gene from Ramazzottius varieornatus
Vector Backbone Description: Backbone Size:2021; Vector Backbone:pCAGGS; Vector Types:Tardigrade expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36693101
Proper citation: RRID:Addgene_205008 Copy
Species: Homo sapiens
Genetic Insert: RORC
Vector Backbone Description: Backbone Marker:Broad Institute Genetic Perturbation Platform; Backbone Size:8264; Vector Backbone:pLX_TRC317; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36608654
Comments: NM_005060.3 Please test multiple small colonies in case of plasmid recombination. To make this collection available in a timely manner, a portion of this collection was not fully sequenced by Addgene. If an Addgene verified full plasmid sequence is not available, please contact us at [email protected] prior to placing an order to request the full sequence.
Proper citation: RRID:Addgene_144446 Copy
Species: Homo sapiens
Genetic Insert: PYL-HDAC5 H1006A
Vector Backbone Description: Backbone Size:5391; Vector Backbone:pAAV-CMV-GFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34494094
Proper citation: RRID:Addgene_114397 Copy
Species: Ramazzottius varieornatus, Tardigrade
Genetic Insert: mCherry-NLS flanked by 1kbp upstream and downstream of the actin gene from R. varieornatus
Vector Backbone Description: Backbone Size:2021; Vector Backbone:pCAGGS; Vector Types:Tardigrade expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36693101
Proper citation: RRID:Addgene_205002 Copy
Species: Ramazzottius varieornatus, Tardigrade
Genetic Insert: RvCAHS3-mEGFP with 1kbp upstream and downstream of the cahs3 gene from Ramazzottius varieornatus
Vector Backbone Description: Backbone Size:2021; Vector Backbone:pCAGGS; Vector Types:Tardigrade expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36693101
Proper citation: RRID:Addgene_205006 Copy
Species: Hypsibius exemplaris, Tardigrade
Genetic Insert: mEGFP-NLS flanked by 1kbp upstream and downstream of the actin gene from Hypsibius exemplaris
Vector Backbone Description: Backbone Size:2021; Vector Backbone:pCAGGS; Vector Types:Tardigrade expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36693101
Proper citation: RRID:Addgene_205003 Copy
Genetic Insert: sfGFP
Vector Backbone Description: Backbone Marker:Sheng et al. 2017; Vector Backbone:pMTL61110; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37154828
Proper citation: RRID:Addgene_201213 Copy
Species: Ramazzottius varieornatus, Tardigrade
Genetic Insert: mEGFP-NLS flanked by 1kbp upstream and downstream of the cahs1 gene from Ramazzottius varieornatus
Vector Backbone Description: Backbone Size:2021; Vector Backbone:pCAGGS; Vector Types:Tardigrade expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36693101
Proper citation: RRID:Addgene_205013 Copy
Species: Hypsibius exemplaris, Tardigrade
Genetic Insert: mEGFP-NLS flanked by 1kbp upstream and downstream of the ef1a gene from Hypsibius exemplaris
Vector Backbone Description: Backbone Size:2021; Vector Backbone:pCAGGS; Vector Types:Tardigrade expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36693101
Proper citation: RRID:Addgene_205012 Copy
Species: Ramazzottius varieornatus, Tardigrade
Genetic Insert: mEGFP-NLS flanked by 1kbp upstream and downstream of the tubulin gene from R. varieornatus
Vector Backbone Description: Backbone Size:2021; Vector Backbone:pCAGGS; Vector Types:Tardigrade expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36693101
Proper citation: RRID:Addgene_205011 Copy
Species: Ramazzottius varieornatus, Tardigrade
Genetic Insert: mEGFP-NLS flanked by 1kbp upstream and downstream of the ubiquitin c gene from R. varieornatus
Vector Backbone Description: Backbone Size:2021; Vector Backbone:pCAGGS; Vector Types:Tardigrade expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36693101
Proper citation: RRID:Addgene_205010 Copy
Species: Rattus norvegicus
Genetic Insert: Rattus rattus biliverdin reductase A flanked with an N-terminal plastid transit peptide and a C-terminal FLAG tag.
Vector Backbone Description: Vector Backbone:pGWB511; Vector Types:Bacterial Expression, Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37285441
Proper citation: RRID:Addgene_203480 Copy
Vector Backbone Description: Backbone Marker:Paul Freemont Lab / iGem; Vector Backbone:pTU2-C-RFP / pSB1C3; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37587063
Comments: Added replications elements for Acinetobacter species into the PstI site; Added Kanamycin resistance cassette into EcoRV site
Proper citation: RRID:Addgene_190124 Copy
Vector Backbone Description: Backbone Marker:Paul Freemont Lab / iGem; Vector Backbone:pTU2-A-RFP / pSB1C3; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37587063
Comments: Added replications elements for Acinetobacter species into the PstI site; Added Kanamycin resistance cassette into EcoRV site
Proper citation: RRID:Addgene_190122 Copy
Vector Backbone Description: Backbone Marker:Paul Freemont Lab / iGem; Vector Backbone:pBP / pSB1C3; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:37587063
Proper citation: RRID:Addgene_190128 Copy
Vector Backbone Description: Backbone Marker:Paul Freemont Lab / iGem; Vector Backbone:pTU2-D-RFP / pSB1C3; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37587063
Comments: Added replications elements for Acinetobacter species into the PstI site; Added Kanamycin resistance cassette into EcoRV site.
NGS assembly indicates that the KanR region is inverted. However, since the plasmid still confers resistance to kanamycin, this mutation is of no concern.
Proper citation: RRID:Addgene_190125 Copy
Species: Synthetic
Genetic Insert: Exon S5 with 49C>A mutation in beta globin splicing minigene
Vector Backbone Description: Backbone Marker:Regev Group (NYU); Vector Backbone:pRegev_ExonSkip_LandingPad; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37796983
Proper citation: RRID:Addgene_204175 Copy
Genetic Insert: MTS-jAspSnFR3
Vector Backbone Description: Vector Backbone:pLenti; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38393319
Comments: Please visit https://doi.org/10.1101/2023.06.27.546775 for bioRxiv preprint.
Proper citation: RRID:Addgene_203487 Copy
Species: Other
Genetic Insert: HA epitope
Vector Backbone Description: Vector Backbone:pHiUGE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31272828
Proper citation: RRID:Addgene_200372 Copy
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