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Species: Arabidopsis thaliana
Genetic Insert: EFR
Vector Backbone Description: Vector Backbone:pEarleyGate103; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_102415 Copy
Species: Synthetic
Genetic Insert: 7SK paired gRNA insert
Vector Backbone Description: Backbone Size:4762; Vector Backbone:pDONR221; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30346403
Proper citation: RRID:Addgene_102440 Copy
Genetic Insert: TetR
Vector Backbone Description: Backbone Size:3878; Vector Backbone:pSC101; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30790525
Comments: Please visit https://www.biorxiv.org/content/early/2017/04/02/123190 for bioRxiv preprint.
Proper citation: RRID:Addgene_102449 Copy
Species: Synechococcus elongatus PCC7942
Genetic Insert: RNAP Beta Prime Subunit with C-terminal FLAG TAG
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29239721
Proper citation: RRID:Addgene_102337 Copy
Genetic Insert: eBFP2
Vector Backbone Description: Backbone Size:1759; Vector Backbone:pJL1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30083609
Proper citation: RRID:Addgene_102640 Copy
Genetic Insert: Minos transposon carrying TetR gene
Vector Backbone Description: Vector Backbone:Lorist6; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:10886410
Proper citation: RRID:Addgene_102537 Copy
Species: Homo sapiens
Genetic Insert: MYC
Vector Backbone Description: Backbone Size:4319; Vector Backbone:pCMV-Tag-4A; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26977881
Proper citation: RRID:Addgene_102625 Copy
Genetic Insert: mTagBFP2
Vector Backbone Description: Backbone Size:1759; Vector Backbone:pJL1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30083609
Proper citation: RRID:Addgene_102638 Copy
Genetic Insert: mTFP1
Vector Backbone Description: Backbone Size:1759; Vector Backbone:pJL1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30083609
Proper citation: RRID:Addgene_102635 Copy
Species: Homo sapiens
Genetic Insert: Homo sapiens RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae) (RAD51), transcript variant 1
Vector Backbone Description: Backbone Marker:Novagen.EMD Millipore; Backbone Size:3719; Vector Backbone:pCOLADuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27671650
Comments: This plasmid was specifically designed to allow high levels of RAD51 expression in E.coli along with the ability to incorporate non-natural amino acids using amber suppressor technology. It was used to incorporate an artificial amino acid to mimic tyrosine phosphorylation (published in Subramanyam S, et. al. (2016). Proceedings of the National Academy of Sciences 113(41):E6045-E6054).
Proper citation: RRID:Addgene_102562 Copy
Species: Other
Genetic Insert: Formylglycine-generating enzyme
Vector Backbone Description: Vector Backbone:pRSF-Duet; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29458769
Comments: FSG gene on PfoI-XhoI fragment from pBAD/myc-hisA Rv0712 (FGE) (Carolyn Bertozzi, Addgene plasmid # 16132) was cloned into pRSF-Duet PfoI-XhoI cut
FGE(rv0712) is nt 4810-5709
Proper citation: RRID:Addgene_102615 Copy
Species: Homo sapiens
Genetic Insert: APPL1
Vector Backbone Description: Backbone Size:4700; Vector Backbone:EGFP-C3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: *Note: Addgene's quality control sequencing finds additional residues KPNSAVDGTAGPGSTGSR appended to the C-terminus of the APPL1 protein. These additional residues are not thought to affect protein function.
Proper citation: RRID:Addgene_102783 Copy
Genetic Insert: J23106:B0034:spisPink:rrnBT1-T7TE
Vector Backbone Description: Backbone Marker:Self-made; Backbone Size:2195; Vector Backbone:derived from pSB1K3; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29293531
Proper citation: RRID:Addgene_102701 Copy
Genetic Insert: J23106:B0034:spisPink:rrnBT1-T7TE
Vector Backbone Description: Backbone Marker:Self-made; Backbone Size:2195; Vector Backbone:derived from pSB1K3; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29293531
Proper citation: RRID:Addgene_102703 Copy
Species: Homo sapiens
Genetic Insert: APPL1
Vector Backbone Description: Backbone Size:4722; Vector Backbone:mCherry-C3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: *Note: Addgene's quality control sequencing finds additional residues KPNSAVDGTAGPGSTGSR appended to the C-terminus of the APPL1 protein. These additional residues are not thought to affect protein function.
Proper citation: RRID:Addgene_102784 Copy
Species: Homo sapiens
Genetic Insert: Rab5a
Vector Backbone Description: Backbone Size:4731; Vector Backbone:EGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: mCherry-C2-Rab5a encodes wild-type human Rab5a,(NM_004162) as a fusion protein with mCherry. Rab5a was inserted between the Eco R1 and Sal 1 restriction sites. mCherry-C2 encodes a monomeric variant of DsRed. The backbone plasmid is pEGFP-C1 (Clontech). The C2 version of mCherry was created by insertion of a short piece of DNA between Xho 1 and EcoR1 restriction sites. In the resulting multiple cloning site, the Sac 1 restriction site has been eliminated and the Eco R1 restriction site is in frame.
Proper citation: RRID:Addgene_102785 Copy
Species: T7 phage
Genetic Insert: T7 RNA polymerase
Vector Backbone Description: Backbone Marker:Qiagen; Vector Backbone:pQE; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29120463
Proper citation: RRID:Addgene_102790 Copy
Genetic Insert: Linker sequence
Vector Backbone Description: Backbone Marker:Self-made; Backbone Size:2267; Vector Backbone:derived from pSB1K3; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29293531
Proper citation: RRID:Addgene_102714 Copy
Genetic Insert: Linker sequence
Vector Backbone Description: Backbone Marker:Self-made; Backbone Size:2267; Vector Backbone:derived from pSB1K3; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29293531
Proper citation: RRID:Addgene_102710 Copy
Species: Homo sapiens
Genetic Insert: EFNA1
Vector Backbone Description: Vector Backbone:pAd Easy1; Vector Types:Adenoviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17079451
Comments: This construct is a adenoviral vector which carries human EFNA1 cDNA and ready to produce adenovirus which express Ephrin-A1 by tranfection into 293A cells followed by PacI digestion.
Proper citation: RRID:Addgene_102724 Copy
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