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Species: Arabidopsis thaliana
Genetic Insert: BR1
Vector Backbone Description: Backbone Size:5515; Vector Backbone:pAAV-CAG; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36324888
Proper citation: RRID:Addgene_192819 Copy
Species: Arabidopsis thaliana
Genetic Insert: AtUbi10 promoter
Vector Backbone Description: Backbone Marker:Self-made; Vector Backbone:pMC-1-ccdB-6; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:36896468
Comments: Please visit https://doi.org/10.1101/2022.10.15.511792 for bioRxiv preprint.
Proper citation: RRID:Addgene_197700 Copy
Species: Arabidopsis thaliana
Genetic Insert: AtU6 promoter for sgRNA assembly
Vector Backbone Description: Backbone Marker:Self-made; Vector Backbone:pMC-1-ccdB-11; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:36896468
Comments: Please visit https://doi.org/10.1101/2022.10.15.511792 for bioRxiv preprint.
Proper citation: RRID:Addgene_197779 Copy
Species: Arabidopsis thaliana
Genetic Insert: Intermediate fragment for assembly of gRNA-TLS2 construct
Vector Backbone Description: Vector Backbone:pENTR4; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36593415
Comments: Please refer to the GO CRISP Cloning Protocol linked above for additional information.
Proper citation: RRID:Addgene_196983 Copy
Species: Arabidopsis thaliana
Genetic Insert: AtNIA1 gRNAs fused to TLS1 tags
Vector Backbone Description: Vector Backbone:pMDC100; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36593415
Comments: Please refer to the GO CRISP Cloning Protocol linked above for additional information.
Proper citation: RRID:Addgene_196980 Copy
Species: Arabidopsis thaliana
Genetic Insert: gRNA expression cassette in pENTR4
Vector Backbone Description: Vector Backbone:pENTR4; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36593415
Comments: Please refer to the GO CRISP Cloning Protocol linked above for additional information.
Proper citation: RRID:Addgene_198729 Copy
Species: Arabidopsis thaliana
Genetic Insert: AtNIA1 gRNAs
Vector Backbone Description: Vector Backbone:pMDC7; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36593415
Comments: Please refer to the GO CRISP Cloning Protocol linked above for additional information.
Proper citation: RRID:Addgene_198728 Copy
Species: Arabidopsis thaliana
Genetic Insert: CIBN
Vector Backbone Description: Backbone Size:7368; Vector Backbone:pCDH-CMV-MCS-EF1-Puro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37217787
Proper citation: RRID:Addgene_188989 Copy
Species: Arabidopsis thaliana
Genetic Insert: CIBN
Vector Backbone Description: Backbone Size:7368; Vector Backbone:pCDH-CMV-MCS-EF1-Puro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37217787
Proper citation: RRID:Addgene_188990 Copy
Species: Arabidopsis thaliana
Genetic Insert: chloroplast inner envelope membrane transit peptide
Vector Backbone Description: Vector Backbone:pGWB405; Vector Types:Bacterial Expression, Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:36398034
Proper citation: RRID:Addgene_213865 Copy
Species: Arabidopsis thaliana
Genetic Insert: MUTEpro_XVE_SCFP3A
Vector Backbone Description: Vector Backbone:pH7m34GW; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:41693245
Proper citation: RRID:Addgene_257227 Copy
Species: Arabidopsis thaliana
Genetic Insert: TMMpro_XVE_SCFP3A
Vector Backbone Description: Vector Backbone:pH7m34GW; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:41693245
Proper citation: RRID:Addgene_257225 Copy
Species: Arabidopsis thaliana
Genetic Insert: FAMApro_XVE_SCFP3A
Vector Backbone Description: Vector Backbone:pH7m34GW; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:41693245
Proper citation: RRID:Addgene_257228 Copy
Species: Arabidopsis thaliana
Genetic Insert: FAMApro_XVE_DBox_SCFP3A
Vector Backbone Description: Vector Backbone:pH7m34GW; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:41693245
Proper citation: RRID:Addgene_257230 Copy
Species: Arabidopsis thaliana
Genetic Insert: Csy4
Vector Backbone Description: Vector Backbone:pTKan-GW-R1R2; Vector Types:Bacterial Expression, Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:28094975
Proper citation: RRID:Addgene_110145 Copy
Species: Arabidopsis thaliana
Genetic Insert: Csy4
Vector Backbone Description: Vector Backbone:pTKan-GW-R1R2; Vector Types:Bacterial Expression, Plant Expression, binary vector; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:28094975
Proper citation: RRID:Addgene_110143 Copy
Species: Arabidopsis thaliana
Genetic Insert: 5X Myc-Spacers-Auxin Inducible Degron for N-terminal attachment
Vector Backbone Description: Backbone Size:2710; Vector Backbone:pUC; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Submitted the 5X Myc-Spacers+Degron sequence (see below) to Genscript for GenePlus Economy synthesis and cloning into the EcoRV site of pUC57.
Proper citation: RRID:Addgene_110913 Copy
Species: Arabidopsis thaliana
Genetic Insert: Phytochrome B
Vector Backbone Description: Backbone Marker:Junichi Miyazaki (Osaka University, Japan); Backbone Size:4870; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29078307
Comments: Expression vector of PhyB-mCherry-HRasCT in mammalian cells. Of note, the expression level of PIF3-mEGFP must be comparable or lower than that of PhyB. We usually co-transfect pCAGGS-PhyB-mCherry-HRasCT and pCAGGS-PIF3-mEGFP plasmids into HeLa cells with lipofection in a 50:1 ratio.
mCherry has a G229L mutation that does not affect function.
Proper citation: RRID:Addgene_100281 Copy
Species: Arabidopsis thaliana
Genetic Insert: PR1 promoter from Arabidopsis thaliana Col-0
Vector Backbone Description: Backbone Marker:Zhao lab at UCSD; Vector Backbone:pHDE-RUBY; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Comments: Addgene's NGS result has discrepancies compared to the depositor's reference sequence: 2bp indel and 1bp indel in PR1 promoter, both in homopolymer regions. The depositor confirms this is not of concern for plasmid function.
Proper citation: RRID:Addgene_255430 Copy
Species: Arabidopsis thaliana
Genetic Insert: RuvA
Vector Backbone Description: Backbone Marker:n/a; Backbone Size:7000; Vector Backbone:pCP3; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:1435721
Comments: Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.
Proper citation: RRID:Addgene_20170 Copy
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