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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pET22b_EcSSB_2 Resource Report Resource Website |
RRID:Addgene_78204 | ssb (W88C) | E. coli | Ampicillin | PMID:20350930 | Please note- The numbering system used was for the mature natural wild-type ssb protein. The N-terminal methionine is quantitatively cleaved off in E. coli and was not numbered so all mutations will be off by 1 nucleotide (e.g., W89C = W88C). The expressed protein is a single-cysteine variant of E. coli single-stranded DNA-binding protein, EcSSB, for subsequent labeling at the cysteine. The adduct with Cy3B maleimide has been used as a biosensor for single-stranded DNA in TIRF microscopy measurements. | Vector Backbone:pET22b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | W88C (please see depositor comment below) | 2026-08-15 01:20:22 | 0 |
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pET22b_EcSSB_1 Resource Report Resource Website |
RRID:Addgene_78203 | ssb (G26C) | E. coli | Ampicillin | PMID:18599625 | Please note: The numbering system used was for the mature natural wild-type ssb protein. The N-terminal methionine is quantitatively cleaved off in E. coli and was not numbered so all mutations will be off by 1 nucleotide (e.g., G27C = G26C). The expressed protein is a single-cysteine variant of E. coli single-stranded DNA-binding protein, EcSSB, for subsequent labeling at the cysteine with one IDCC (N-[2-(iodoacetamido)ethyl]-7-diethylaminocoumarin-3-carboxamide) per subunit. This adduct is then used as a fluorescent single-stranded DNA (ssDNA) biosensor, having ~6-fold fluorescence increase on binding ssDNA with very high affinity. | Vector Backbone:pET22b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | G26C (please see depositor comment below) | 2026-08-15 01:20:22 | 0 |
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pET22b_PstS_3 Resource Report Resource Website |
RRID:Addgene_78200 | pstS (A17C,I76G,A197C) | E. coli | Ampicillin | PMID:26199994 | Please note- The numbering system used was for the mature natural wild-type PstS protein. The plasmid produces an extra N-terminal methionine so all mutations will be off by 1nt (e.g., A197C =A198C) PstS ORF encoding the mature E. coli (A17C, I76G, A197C)PstS protein (PBPw) containing a stop codon at the end of the gene in pET22b (inserted between Nde1 and Xho1 sites in the MCS). Expression produces MEA at the N-terminus of the mature PstS: So there is an extra M at the N-terminus over the natural, mature PstS. A stop codon was also added at the end of the sequence BEFORE the plasmid-derived His tag. This gives PLY at the C-terminus at the C-terminus of the expressed protein, as in the natural PstS. The amino-acid numbering has been left as based on positions in the natural, mature protein. Usage The expressed protein is a weak-binding variant of E. coli phosphate binding protein, PstS. It contains two cysteines (A17C, A197C) and a mutation to weaken binding (I76G). The gene encodes the mature protein without the N-terminal signal peptide, but with an N-terminal methionine. Subsequently the cysteines are labeled with 2 6-iodoactemidotetramethylrhodamines per molecule. This adduct is then used as a phosphate biosensor, having up to 9-fold fluorescence intensity increase on binding inorganic phosphate, with a low dissociation constant so inorganic phosphate can be measured in the tens of micromolar range. | Vector Backbone:pET22b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | A17C,I76G,A197C (please see depositor comment below) | 2026-08-15 01:20:22 | 0 |
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pHis17_ParM _2 Resource Report Resource Website 1+ mentions |
RRID:Addgene_78202 | parM (K33A D63C T174A T175N D224C C287A) | E. coli | Ampicillin | PMID:20158267 | The plasmid is used to express the plasmid segregation protein, ParM, from E. col. The protein is modified for use as the scaffold of an ADP biosensor, ATR-ParM. Two cysteine mutations are for label attachment (D63C and D224C); there is a mutation to inhibit filament formation (K33A), two mutations to weaken ATP binding (T174A and T175N), a mutation to change the exposed cysteine (C287A) and a C-terminal His6 tag to aid purification. When labelled with two iodoacetamidotetramethylrhodamine (IATR) fluorophores, this biosensor has a fluorescence intensity change of up to 20-fold and can be used to measure ADP in the range of hundreds of nanomolar to ~100 μM, in the presence of up to millimolar ATP. The biosensor can also be used for measurement of other nucleoside diphosphates e.g. GDP, UDP and others. | Vector Backbone:pHis17; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | K33A D63C T174A T175N D224C C287A | 2026-08-15 01:20:22 | 3 |
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pHis17_ParM _1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_78201 | parM (K33A I27C T174A T175N C287A) | E. coli | Ampicillin | PMID:19801632 | The plasmid is used to express the plasmid segregation protein, ParM, from E. col. The protein is modified for use as the scaffold of an ADP biosensor, MDCC-ParM. A single cysteine mutation is for label attachment (I27C); there is a mutation to inhibit filament formation (K33A), two mutations to weaken ATP binding (T174A and T175N), a mutation to change the exposed cysteine (C287A) and a C-terminal His6 tag to aid purification. When labelled with a single N-[2-(1-maleimidyl)ethyl]-7-diethylaminocoumarin-3-carboxamide (MDCC) fluorophore, this biosensor has a fluorescence intensity change of up to 4-fold and can be used to measure ADP in the range of tens of nanomolar to tens of micromolar in the presence of up to~200 μM ATP. | Vector Backbone:pHis17; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | K33A I27C T174A T175N C287A | 2026-08-15 01:20:22 | 5 |
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pET22b_PstS_2 Resource Report Resource Website |
RRID:Addgene_78199 | pstS (A17C,A197C) | E. coli | Ampicillin | PMID:26199994 | Please note- The numbering system used was for the mature natural wild-type PstS protein. The plasmid produces an extra N-terminal methionine so all mutations will be off by 1nt (e.g., A197C =A198C) The expressed protein is a double-cysteine variant of E. coli phosphate binding protein. The gene encodes the mature protein without the N-terminal signal peptide, but with an N-terminal methionine, for subsequent labeling at the cysteines with two 6-iodoacetamidotetramethylrhodamines per molecule. This adduct is then used as a fluorescent phosphate biosensor, having 10-15-fold fluorescence increase on binding inorganic phosphate with very high affinity. | Vector Backbone:pET22b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | A17C; A197C (please see depositor comment below) | 2026-08-15 01:20:22 | 0 |
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MetNI (E166Q in MetN) Resource Report Resource Website |
RRID:Addgene_78320 | MetI | E. coli | Ampicillin | PMID:22095702 | Backbone Marker:Novagen; Backbone Size:5791; Vector Backbone:pET19b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:20:23 | 0 | ||
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MetNI (Lm) Resource Report Resource Website |
RRID:Addgene_78316 | MetI | E. coli | Ampicillin | PMID:18621668 | Backbone Marker:Novagen; Backbone Size:5791; Vector Backbone:pET19b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:20:23 | 0 | ||
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pZA31-sulA-GFP Resource Report Resource Website 1+ mentions |
RRID:Addgene_78493 | GFPmut2 | E. coli | Chloramphenicol | PMID:27060147 | Vector Backbone:pZA31-Luc; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol | 2026-08-15 01:20:24 | 2 | ||
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pCOLA-T7-YhjH Resource Report Resource Website 1+ mentions |
RRID:Addgene_79165 | yhjH | E. coli | Kanamycin | PMID:27382070 | Backbone Marker:Novagen; Backbone Size:3719; Vector Backbone:pCOLADuet; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:20:29 | 1 | ||
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pETDuet-T7-Ct-YhjH Resource Report Resource Website 1+ mentions |
RRID:Addgene_79167 | yhjH | E. coli | Ampicillin | PMID:27382070 | Backbone Marker:Novagen; Backbone Size:5420; Vector Backbone:pETDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:20:29 | 1 | ||
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pB33eCPX Resource Report Resource Website 1+ mentions |
RRID:Addgene_23336 | Enhanced Circularly Permuted Outer Membrane Protein X | E. coli | Chloramphenicol | PMID:18480093 | Backbone Size:5400; Vector Backbone:pBAD33; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol | 2026-08-15 01:12:19 | 8 | ||
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ykgMO-IGS-sfGFP Resource Report Resource Website |
RRID:Addgene_207231 | ykgMO-IGS | E. coli | Chloramphenicol | PMID:37523538 | Backbone Marker:p15A ori plasmid; Backbone Size:4770; Vector Backbone:pJBL5034; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol | 2026-08-15 01:28:55 | 0 | ||
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ykgMO-IGS-LuxCDABE Resource Report Resource Website |
RRID:Addgene_207232 | ykgMO-IGS | E. coli | Chloramphenicol | PMID:37523538 | Backbone Marker:p15A ori plasmid; Backbone Size:2269; Vector Backbone:pJBL5034; Vector Types:Bacterial Expression, Luciferase, Synthetic Biology; Bacterial Resistance:Chloramphenicol | 2026-08-15 01:28:54 | 0 | ||
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COC053 Resource Report Resource Website |
RRID:Addgene_209131 | Enzyme BirA | E. coli | Ampicillin | PMID:9887208 | Vector Backbone:pGEX-2T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:29:07 | 0 | ||
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Strep-TGT-His Resource Report Resource Website |
RRID:Addgene_206495 | E. coli tRNA Guanine Transglycosylase | E. coli | Ampicillin | PMID:36988146 | Backbone Marker:NEB; Backbone Size:5874; Vector Backbone:(T7)-2 Vector; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:27:53 | 0 | ||
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pAAV-CAG-tTA Resource Report Resource Website 1+ mentions |
RRID:Addgene_193338 | tTA2 | E. coli | Ampicillin | PMID:38918382 | Please visit https://www.biorxiv.org/content/10.1101/2022.10.20.512984v1 for bioRxiv preprint. | Vector Backbone:AAV with CAG promter; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin | 2026-08-15 01:27:37 | 1 | |
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pDA303 Resource Report Resource Website |
RRID:Addgene_195199 | TetR | E. coli | Kanamycin | PMID:36246369 | Vector Backbone:pQE80; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:27:43 | 0 | ||
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pTrc-CysGA-Im7 F84A Resource Report Resource Website |
RRID:Addgene_201082 | CysGA-Im7 F84A | E. coli | Ampicillin | PMID:33753520 | Backbone Size:3904; Vector Backbone:pTrc-99a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | Im7 F84A | 2026-08-15 01:27:49 | 0 | |
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pTrc-CysGA-Im7 WT Resource Report Resource Website |
RRID:Addgene_201081 | CysGA-Im7 | E. coli | Ampicillin | PMID:33753520 | Backbone Size:3904; Vector Backbone:pTrc-99a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:27:48 | 0 |
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