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Species: Other
Genetic Insert: Rluc
Vector Backbone Description: Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19383768
Comments: Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_146090 Copy
Species: Other
Genetic Insert: GFP52
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Comments: Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_146099 Copy
Species: Other
Genetic Insert: GFP-PTC
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18974281
Comments: Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_146154 Copy
Species: Other
Genetic Insert: ccdb
Vector Backbone Description: Vector Backbone:I-SceI; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:32709891
Proper citation: RRID:Addgene_141067 Copy
Species: Other
Genetic Insert: loxp-DsRed-loxp-eGFP
Vector Backbone Description: Backbone Marker:Eric Campeau & Paul Kaufman (Addgene plasmid # 17294); Vector Backbone:pLenti CMV/TO Zeo DEST (644-1) ; Vector Types:Lentiviral; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:31649238
Comments: loxp-DsRed-loxp-eGFP was PCR amplified from pMSCV-loxp-DsRed-loxp-eGFP-Puro-WPRE (Addgene plasmid #32702) using primers containing Sal1 and Not1 restriction enzyme sites. The amplified PCR fragment was digested with Sal1 and Not1 and ligated into the pENTRA1 vector cut with Sal1 and Not1. The Gateway SystemTM (Invitrogen) was used to recombine the pENTR1A shuttle vector with pLenti CMV/TO Zeo DEST (644-1) (#17294, Addgene) generating lentiviral vector 1.
Proper citation: RRID:Addgene_141148 Copy
Species: Other
Genetic Insert: 4xHRE-MinTK-CRE
Vector Backbone Description: Backbone Marker:Didier Trono (Addgene plasmid # 12252); Vector Backbone:pRRLSIN.cPPT.PGK-GFP.WPRE; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31649238
Proper citation: RRID:Addgene_141144 Copy
Species: Other
Genetic Insert: A7 3nY tRNA synthetase
Vector Backbone Description: Vector Backbone:pAcBac; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31117397
Proper citation: RRID:Addgene_141173 Copy
Species: Other
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:pET-45(b+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32386547
Proper citation: RRID:Addgene_145279 Copy
Species: Other
Genetic Insert: CasPhi-3
Vector Backbone Description: Vector Backbone:Custom; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32675376
Proper citation: RRID:Addgene_158802 Copy
Species: Other
Genetic Insert: CasPhi-1
Vector Backbone Description: Vector Backbone:Custom; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32675376
Proper citation: RRID:Addgene_158800 Copy
Species: Other
Genetic Insert: Sth1 sgRNA scaffold
Vector Backbone Description: Vector Backbone:custom design; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:31992616
Comments: insert sizes: 171 bp and 3366 bp
Proper citation: RRID:Addgene_158719 Copy
Species: Other
Genetic Insert: eforCP (for crRNA cloning)
Vector Backbone Description: Vector Backbone:Pacyc184; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28646112
Proper citation: RRID:Addgene_158711 Copy
Species: Other
Genetic Insert: dCasPhi-3 (D413A)
Vector Backbone Description: Vector Backbone:pRSF-Duet1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32675376
Comments: Please note there is a silent mutation in the dCasPhi-3 coding sequence that does not alter plasmid function.
Proper citation: RRID:Addgene_158799 Copy
Species: Other
Genetic Insert: CasPhi-3
Vector Backbone Description: Vector Backbone:pRSF-Duet1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32675376
Proper citation: RRID:Addgene_158796 Copy
Species: Other
Genetic Insert: CasPhi-1
Vector Backbone Description: Vector Backbone:pRSF-Duet1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32675376
Proper citation: RRID:Addgene_158794 Copy
Species: Other
Genetic Insert: Sth1 sgRNA scaffold
Vector Backbone Description: Vector Backbone:custom design; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:31992616
Comments: insert sizes: 171 bp and 3366 bp
Proper citation: RRID:Addgene_158720 Copy
Species: Other
Genetic Insert: AsCpf1-HA-2A-GFP
Vector Backbone Description: Vector Backbone:pCDNA3.1; Vector Types:Mammalian Expression, Multicistronic vector with both CAGGS promoter-driven AsCpf1 and U6 promoter-driven single guide RNA (sgRNA); Bacterial Resistance:Ampicillin
Defining Citation: PMID:33205073
Proper citation: RRID:Addgene_159281 Copy
Species: Other
Genetic Insert: tdTomato
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:Mammalian Expression, AAV, S1 knockin donor vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29599125
Proper citation: RRID:Addgene_159275 Copy
Species: Other
Genetic Insert: mScarlet-I
Vector Backbone Description: Backbone Marker:Naldini Lab; Backbone Size:6782; Vector Backbone:pLenti-MP2; Vector Types:Mammalian Expression, Lentiviral, Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: The codons were optimized to the Human codon usage
Proper citation: RRID:Addgene_159172 Copy
Species: Other
Genetic Insert: OsTIR
Vector Backbone Description: Backbone Size:8811; Vector Backbone:pKLV-EF1amCherry; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32764680
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.04.25.054049v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_159296 Copy
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