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Species: Synthetic
Genetic Insert: Double SapI flanked mTurquoise2
Vector Backbone Description: Backbone Size:1969; Vector Backbone:Minimalistic Amp + ColE1 backbone; Vector Types:Targeting vector backbone; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35089911
Proper citation: RRID:Addgene_169222 Copy
Species: Synthetic
Genetic Insert: nsp12-His6-3xFlag/nsp7-Linker-nsp8
Vector Backbone Description: Vector Backbone:pBIG2abc (biGBac); Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:34198323
Comments: Baculovirus generation using Tn7 transposition (Bac-to-Bac).
Proper citation: RRID:Addgene_169184 Copy
Species: Synthetic
Genetic Insert: nsp12-His6-3xFlag/nsp7/nsp8
Vector Backbone Description: Vector Backbone:pBIG2abc (biGBac); Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:34198323
Comments: Baculovirus generation using Tn7 transposition (Bac-to-Bac).
Proper citation: RRID:Addgene_169185 Copy
Species: Synthetic
Genetic Insert: 14His-SUMO-nsp10-GSGSGS-nsp16
Vector Backbone Description: Vector Backbone:pK27SUMO; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34198328
Proper citation: RRID:Addgene_169191 Copy
Species: Synthetic
Genetic Insert: 14His-SUMO-nsp3_E746-K1060 (pp1ab_E1564-K1878)
Vector Backbone Description: Vector Backbone:pK27SUMO; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34198325
Proper citation: RRID:Addgene_169193 Copy
Species: Synthetic
Genetic Insert: Double SapI flanked Dendra2-P2A-Puro
Vector Backbone Description: Backbone Size:1969; Vector Backbone:Minimalistic Amp + ColE1 backbone; Vector Types:Targeting vector backbone; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35089911
Proper citation: RRID:Addgene_169217 Copy
Species: Synthetic
Genetic Insert: Double SapI flanked mScarlet
Vector Backbone Description: Backbone Size:1969; Vector Backbone:Minimalistic Amp + ColE1 backbone; Vector Types:Targeting vector backbone; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35089911
Proper citation: RRID:Addgene_169218 Copy
Species: Synthetic
Genetic Insert: Double SapI flanked mScarlet
Vector Backbone Description: Backbone Size:1969; Vector Backbone:Minimalistic Amp + ColE1 backbone; Vector Types:Targeting vector backbone; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35089911
Proper citation: RRID:Addgene_169219 Copy
Species: Synthetic
Genetic Insert: ZnGreen1
Vector Backbone Description: Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27539450
Comments: When selecting zinc biosensors for your particular application, please pay attention to the Kd. The Kd of the sensor has to match the concentration range of your particular experiments.
Proper citation: RRID:Addgene_169470 Copy
Species: Synthetic
Genetic Insert: pMag-Dre247
Vector Backbone Description: Vector Backbone:Adapted from pT2/BH; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33318209
Proper citation: RRID:Addgene_169705 Copy
Species: Synthetic
Genetic Insert: Dre246-nMag-T2A-pMag-Dre247
Vector Backbone Description: Vector Backbone:Adapted from px330; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33318209
Proper citation: RRID:Addgene_169706 Copy
Species: Synthetic
Genetic Insert: a double-floxed inverted Dre246-nMag-T2A-pMag-Dre247
Vector Backbone Description: Vector Backbone:Adapted from px330; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33318209
Proper citation: RRID:Addgene_169708 Copy
Species: Synthetic
Genetic Insert: anti-caffeine nanobody
Vector Backbone Description: Backbone Size:4900; Vector Backbone:mEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33552855
Proper citation: RRID:Addgene_169685 Copy
Species: Synthetic
Genetic Insert: tetR-P2A-eGFP-miR(FF4) target-miR(FF4)
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:8594; Vector Backbone:pcDNA5/FRT; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34226556
Comments: Note that although the plasmid encodes a hygromycin resistance gene, it is not expressed from the plasmid. It is only expressed after Flp recombinase-mediated chromosomal integration of the plasmid.
Proper citation: RRID:Addgene_169734 Copy
Species: Synthetic
Genetic Insert: miR(FF4) target-tetR-P2A-eGFP-miR(FF4) target-miR(FF4)
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:8585; Vector Backbone:pcDNA5/FRT; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34226556
Comments: Note that although the plasmid encodes a hygromycin resistance gene, it is not expressed from the plasmid. It is only expressed after Flp recombinase-mediated chromosomal integration of the plasmid.
Proper citation: RRID:Addgene_169735 Copy
Species: Synthetic
Genetic Insert: tetR-P2A-eGFP-miR(FF4) target-miR(FF4)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:6113; Vector Backbone:pcDNA5/FRT; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34226556
Proper citation: RRID:Addgene_169731 Copy
Species: Synthetic
Genetic Insert: miR(FF4) target-tetR-P2A-eGFP-miR(FF4) target-miR(FF4)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:6104; Vector Backbone:pcDNA5/FRT; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34226556
Comments: Note that although the plasmid encodes a hygromycin resistance gene, it is not expressed from the plasmid. It is only expressed after Flp recombinase-mediated chromosomal integration of the plasmid.
Proper citation: RRID:Addgene_169732 Copy
Species: Synthetic
Genetic Insert: miR(FF4) target-tetR-P2A-eGFP-miR(FF4) target-miR(FF4)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:13287; Vector Backbone:pCEP4; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34226556
Comments: Please note the OriP sequence contains a deletion that is also found in Addgene plasmid (pCEP4-CXCR4) which was used as the backbone.
Proper citation: RRID:Addgene_169738 Copy
Species: Synthetic
Genetic Insert: SpCas9-N57
Vector Backbone Description: Backbone Marker:Addgene # 52962; Vector Backbone:pCAG-spCas9; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32986839
Proper citation: RRID:Addgene_169920 Copy
Species: Synthetic
Genetic Insert: dCjCas9
Vector Backbone Description: Backbone Marker:Addgene #89752; Vector Backbone:pRGEN-CMV-CjCas9; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33751124
Proper citation: RRID:Addgene_169912 Copy
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