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Species: Chloramphenicol resistance gene (CmR) - ccdB gene
Genetic Insert: Chloramphenicol resistance gene (CmR) - ccdB gene
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:9998; Vector Backbone:pLenti6/UbC/V5-Dest; Vector Types:Mammalian Expression, Lentiviral, Other, Gateway; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20132838
Comments: V5 tag deleted
Proper citation: RRID:Addgene_24177 Copy
Species: reporter gene mCherry
Vector Backbone Description: Backbone Marker:Scott Barolo; Backbone Size:10462; Vector Backbone:pGreen H-Pelican; Vector Types:reporter construct; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20506180
Comments: AmpR in backbone; CmR in gateway RfA cassette
Proper citation: RRID:Addgene_24567 Copy
Genetic Insert: Gateway(TM) cassette
Vector Backbone Description: Backbone Size:0; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20305087
Comments: The mouse PGK promoter was PCR-amplified and cloned into pLD-puro-CnVA (Plasmid #24587) to generate pLD-puro-PGKnVA.
VA= versatile affinity tag, 3XFlag, 6XHis+StrepIII
Proper citation: RRID:Addgene_24589 Copy
Genetic Insert: Gateway(TM) cassette
Vector Backbone Description: Backbone Size:0; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20305087
Comments: The CMV promoter from the pLJM1 vector (Addgene) was PCR amplified and cloned into pLD-puro-EnVA using NdeI/NheI to generate pLD-puro-CnVA.
pLD-puro-EnVA (pLD-puromycin resistance- EF1alpha, N-terminal VA tag) was generated as described in the paper from pLKO.1
VA tag= versatile affinity tag, 3XFlag+2XTev+6XHis+2XStrepIII
Proper citation: RRID:Addgene_24587 Copy
Genetic Insert: Gateway(TM) cassette
Vector Backbone Description: Backbone Size:0; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20305087
Comments: VA= Versatile Affinity tag, 3XFlag+6XHis+StrepIII
Proper citation: RRID:Addgene_24592 Copy
Genetic Insert: Gateway(TM) cassette
Vector Backbone Description: Backbone Size:0; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20305087
Comments: pLD-hygro-EnVM was constructed by subcloning the hygromycin resistance gene from the pLJM6 vector (J Moffat, unpublished) into the pLD-puro-EnFH vector using SpeI/NsiI and subsequently subcloning a gene synthesized V5-Myc (VM) tag (Bio Basic Inc.) from the pUC57 host vector.
pLD-puro-EnFH (pLD-puromycin resistance- EF1alpha, N-terminus triple Flag-6-His) was generated from pLKO.1 (see paper for details).
Proper citation: RRID:Addgene_24590 Copy
Vector Backbone Description: Backbone Size:9249; Vector Backbone:p156RRL-sinPPT-CMV-GFP-PRE/Nhe I; Vector Types:Lentiviral, RNAi, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:19657394
Comments: Please note that there are a few gaps in alignment between Addgene's quality control sequence and the depositor's reference sequence. These mismatches are in backbone regions and should not affect function.
Proper citation: RRID:Addgene_17390 Copy
Vector Backbone Description: Backbone Size:8784; Vector Backbone:p156RRL-sinPPT-CMV-GFP-PRE/Nhe I; Vector Types:Lentiviral, RNAi, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:19657394
Comments: Please note that there are a few gaps in alignment between Addgene's quality control sequence and the depositor's reference sequence. These mismatches are in backbone regions and should not affect function.
Proper citation: RRID:Addgene_17391 Copy
Vector Backbone Description: Backbone Size:9515; Vector Backbone:pQCXIH; Vector Types:Mammalian Expression, Retroviral, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:19657394
Proper citation: RRID:Addgene_17394 Copy
Vector Backbone Description: Backbone Size:8614; Vector Backbone:pQCXIB; Vector Types:Mammalian Expression, Retroviral, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:19657394
Proper citation: RRID:Addgene_17400 Copy
Vector Backbone Description: Backbone Size:8562; Vector Backbone:pQCXIZ; Vector Types:Mammalian Expression, Retroviral, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:19657394
Proper citation: RRID:Addgene_17401 Copy
Vector Backbone Description: Backbone Size:9331; Vector Backbone:p156RRL-sinPPT-CMV-GFP-PRE/Nhe I; Vector Types:Mammalian Expression, Lentiviral, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:19657394
Comments: There are 2 gaps between depositor's reference sequence and Addgene's quality control sequence. The gaps are in vector regions only and do not affect any of the features.
Proper citation: RRID:Addgene_17451 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA4/TO; Vector Types:Mammalian Expression, TALEN; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24324157
Comments: This destination vector for TALEs is compatible with the Zhang Lab TALE Toolbox.
Proper citation: RRID:Addgene_49631 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA4/TO; Vector Types:Mammalian Expression, TALEN; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24324157
Comments: This destination vector for TALEs is compatible with the Zhang Lab TALE Toolbox.
Proper citation: RRID:Addgene_49630 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA4/TO; Vector Types:Mammalian Expression, TALEN; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24324157
Comments: This destination vector for TALEs is compatible with the Zhang Lab TALE Toolbox.
Proper citation: RRID:Addgene_49624 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA4/TO; Vector Types:Mammalian Expression, TALEN; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24324157
Comments: This destination vector for TALEs is compatible with the Zhang Lab TALE Toolbox.
Proper citation: RRID:Addgene_49626 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA4/TO; Vector Types:Mammalian Expression, TALEN; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24324157
Comments: This destination vector for TALEs is compatible with the Zhang Lab TALE Toolbox.
Proper citation: RRID:Addgene_49625 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2400; Vector Backbone:pDESTR4-R3; Vector Types:Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24820376
Comments: See www.wormbuilder.org for updated protocols.
Proper citation: RRID:Addgene_51481 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2400; Vector Backbone:pDESTR4-R3; Vector Types:Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24820376
Comments: See www.wormbuilder.org for updated protocols.
Proper citation: RRID:Addgene_51483 Copy
Species: Caenorhabditis elegans
Genetic Insert: unc-119 rescuing fragment / pie-1promoter-GFP- gateway destination cassette B -pie-1 3’
Vector Backbone Description: Backbone Size:16320; Vector Backbone:n/a; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:16499902
Comments: Use ONLY DB3.1 to transform this plasmid. DH5 alpha does not work (it has a death gene).
Proper citation: RRID:Addgene_22720 Copy
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