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Species: Rattus norvegicus
Genetic Insert: apolipoprotein B mRNA editing enzyme, catalytic polypeptide 1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25806564
Proper citation: RRID:Addgene_112857 Copy
Species: Rattus norvegicus
Genetic Insert: rat Kv3.1
Vector Backbone Description: Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23233530
Comments: This plasmid contains a mutation an I350N in Kv3.1 compared to the reference NP_036988.1.
Proper citation: RRID:Addgene_113055 Copy
Species: Rattus norvegicus
Genetic Insert: Two gRNAs for rat TH
Vector Backbone Description: Backbone Marker:NIDA OTTC; Backbone Size:6000; Vector Backbone:pOTTC407; Vector Types:Mammalian Expression, AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30792150
Proper citation: RRID:Addgene_113155 Copy
Species: Rattus norvegicus
Genetic Insert: gRNA for rat TH
Vector Backbone Description: Backbone Marker:NIDA OTTC; Backbone Size:6000; Vector Backbone:pOTTC407; Vector Types:Mammalian Expression, AAV, Cre/Lox, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30792150
Proper citation: RRID:Addgene_113158 Copy
Species: Rattus norvegicus
Genetic Insert: Two gRNAs for rat MANF
Vector Backbone Description: Backbone Marker:NIDA OTTC; Backbone Size:6000; Vector Backbone:pOTTC407; Vector Types:Mammalian Expression, AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30792150
Proper citation: RRID:Addgene_113157 Copy
Species: Rattus norvegicus
Genetic Insert: IRS-3
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript SK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11113178
Comments: (Kahn lab plasmid name = pBS IRS3)
Proper citation: RRID:Addgene_11361 Copy
Species: Rattus norvegicus
Genetic Insert: NK1R
Vector Backbone Description: Backbone Marker:Chris Richie; Backbone Size:5236; Vector Backbone:pOTTC385; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31242442
Proper citation: RRID:Addgene_102935 Copy
Species: Rattus norvegicus
Genetic Insert: LAMP1
Vector Backbone Description: Vector Backbone:pLJC5; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29074583
Comments: LAMP1-RFP was cloned from Plasmid #34611
Proper citation: RRID:Addgene_102932 Copy
Species: Rattus norvegicus
Genetic Insert: KIF1A
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:9902; Vector Backbone:peGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27658622
Comments: The rat eGFP-KIF1A was a kind gift from Gary Banker (OHSU).
The tdTomato-KIF1A plasmid was constructed by restriction site removal of the eGFP from the eGFP-KIF1A plasmid and insertion of tdTomato.
Addgene NGS results found S33N within the KIF1A translation compared to NCBI reference sequence XP_017459420.1
Proper citation: RRID:Addgene_102850 Copy
Species: Rattus norvegicus
Genetic Insert: GLUT2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8626492
Comments: cDNA clone isolated from a rat islet cDNA library constructed in pcDNA-1. The full length insert was excised by KpnI/XbaI and subcloned into pcDNA3.
Proper citation: RRID:Addgene_14946 Copy
Species: Rattus norvegicus
Genetic Insert: Rat insulin promoter (RIP)
Vector Backbone Description: Backbone Size:6700; Vector Backbone:modified pBluescript II KS; Vector Types:Mammalian Expression, Cre/Lox, for making transgenic constructs; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15129273
Comments: The CRE-ER(TM) protein requires tamoxifen (TM) to catalyze LoxP site-mediated excision.
The RIP–CreER construct was generated by fusing a 0.66-kilobase SmaI–HindIII fragment of the RIP2 promoter to a minimal hsp68 promoter, and placing the chimaeric promoter upstream of CreER(TM).
See Author's Map for more information.
Proper citation: RRID:Addgene_15029 Copy
Species: Rattus norvegicus
Genetic Insert: JNK3
Vector Backbone Description: Backbone Marker:Bruce Mayer Lab; Backbone Size:6000; Vector Backbone:pEBG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_15005 Copy
Species: Rattus norvegicus
Genetic Insert: Hes1
Vector Backbone Description: Backbone Marker:Gary Nolan, Stanford; Backbone Size:0; Vector Backbone:pBABE-MN-IRES-E-GFP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10839357
Comments: Dominant negative Hes1.
Please note that the vector backbone pBABE-MN-IRES-E-GFP (also known as pBMN-I-GFP, Addgene plasmid# 1736) is extremely slow growing and may need 2 days to grow.
Proper citation: RRID:Addgene_15134 Copy
Species: Rattus norvegicus
Genetic Insert: insulin II promoter
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4100; Vector Backbone:pTimer-1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15129273
Comments: See Author's Map for more information.
Proper citation: RRID:Addgene_15109 Copy
Species: Rattus norvegicus
Genetic Insert: CREB S133A
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3500; Vector Backbone:pM; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16125054
Comments: Wild-type CREB S133A fused to the DNA binding domain of Gal4. The plasmids were generated by cloning the CREB S133A as an XhoI/XbaI fragment into the SalI/XbaI sites of pM.
Proper citation: RRID:Addgene_15222 Copy
Species: Rattus norvegicus
Genetic Insert: CREB
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3500; Vector Backbone:pM; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16125054
Comments: Wild-type CREB fused to the DNA binding domain of Gal4. The plasmids were generated by cloning the cDNA of CREB as an XhoI/XbaI fragment into the SalI/XbaI sites of pM.
Proper citation: RRID:Addgene_15221 Copy
Species: Rattus norvegicus
Genetic Insert: PHAS-I
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:4671; Vector Backbone:pET14b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12665511
Comments: Full-length PHASI with a His tag. See author's map for detailed information.
Proper citation: RRID:Addgene_15679 Copy
Species: Rattus norvegicus
Genetic Insert: rApoI
Vector Backbone Description: Vector Backbone:None; Vector Types:; Bacterial Resistance:Streptomycin
Defining Citation: PMID:29991261
Comments: Esherichia coli strain that expresses a deactivated variant of MutaT7, a fusion of rat APOBEC1 and the T7 RNA polymerase. Genotype: DH10B Δung ΔmotAB ΔcsgABCDEFG [PlacI<>Ptac] Δ(araA-leu)7697::[PA1lacO-Tenth drApo1-T7]
Proper citation: RRID:Addgene_156461 Copy
Species: Rattus norvegicus
Genetic Insert: LAMP1
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33097540
Comments: Existing fluorescent protein in plasmid Adggene# 56618 was replaced with mGold.
"N-20" of the name of the plasmid indicates the position of the tag (N- or C-terminal with respect to the fluorescent protein) and the number of residues in the linker between the two proteins. Please note that the mVenus annotation in the plasmid map is incorrect, as this plasmid encodes mGold.
Proper citation: RRID:Addgene_158006 Copy
Species: Rattus norvegicus
Genetic Insert: rat PAM3
Vector Backbone Description: Backbone Marker:Cornelia Gorman; Backbone Size:5000; Vector Backbone:pCIS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8294504
Comments: Please note: Plasmid contains a Valine 221 deletion in rPAM3 compared to the NCBI reference sequence AAC05608.1. This deletion is not known to affect protein activity.
Proper citation: RRID:Addgene_145803 Copy
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