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Genetic Insert: GFP
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript SK(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22479386
Proper citation: RRID:Addgene_36874 Copy
Species: Rattus norvegicus
Genetic Insert: beta-arrestin2
Vector Backbone Description: Backbone Size:5015; Vector Backbone:pcDNA3.1 zeo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16687412
Proper citation: RRID:Addgene_36917 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: 3x mCherry
Vector Backbone Description: Backbone Size:9951; Vector Backbone:pSinRep5; Vector Types:Mammalian Expression, Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22327349
Proper citation: RRID:Addgene_36914 Copy
Species: Rattus norvegicus
Genetic Insert: beta-arrestin1
Vector Backbone Description: Backbone Size:5015; Vector Backbone:pcDNA3.1 zeo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16687412
Proper citation: RRID:Addgene_36916 Copy
Species: HIV-I
Genetic Insert: fNL43-dE-EGFP
Vector Backbone Description: Backbone Size:2686; Vector Backbone:pUC18; Vector Types:Mammalian Expression, Lentiviral, Retroviral; Bacterial Resistance:Ampicillin
Comments: Plasmid region from nt9236 to nt308 is derived from pNL43dSL1.
Proper citation: RRID:Addgene_36865 Copy
Species: HIV-I
Genetic Insert: NL43-dGPE-EGFP
Vector Backbone Description: Backbone Size:2686; Vector Backbone:pUC18; Vector Types:Mammalian Expression, Lentiviral, Retroviral; Bacterial Resistance:Ampicillin
Comments: This plasmid is based on pfNL43-dE-EGFP.
Addgene's sequencing results identified 4 nucleotide mismatches at bp# 1634 & 2223-2225 when compared to the full plasmid sequence provided by the depositing laboratory, are remnants of the deleted pol gene and are not a concern for the function of the plasmid. According to the depositing laboratory, this plasmid still produces infectious virus when gag, pol and env are supplied in trans.
Proper citation: RRID:Addgene_36866 Copy
Species: Homo sapiens
Genetic Insert: WDR5
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:7325; Vector Backbone:pET28-MHL (Addgene plasmid 26096); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_36900 Copy
Species: Danio rerio
Genetic Insert: Hox B7a
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3000; Vector Backbone:pGEM-T; Vector Types:Bacterial Expression, Riboprobe Synthesis; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9425136
Comments: Please note that Addgene's sequencing results indicate the Hox B7a insert in this plasmid starts at amino acid 143 when compared to GenBank entry NP_001108563.1. The depositing laboratory believes these are allelic differences common with outbred strains and this is the exact plasmid they used in the associated publication.
Proper citation: RRID:Addgene_36861 Copy
Species: Danio rerio
Genetic Insert: Hox B8a
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3000; Vector Backbone:pGEM-T; Vector Types:Bacterial Expression, Riboprobe Synthesis; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9425136
Comments: Please note that Addgene's sequencing results indicate the Hox B8a insert in this plasmid has a D238E mutation when compared to GenBank entry NP_571195.1. The depositing laboratory believes these are allelic differences common with outbred strains and this is the exact plasmid they used in the associated publication.
Proper citation: RRID:Addgene_36862 Copy
Genetic Insert: T (i.e., ATG, start codon as a first exon)
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript SK(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22479386
Comments: This is the 'LNL' version of the plasmid. It contains loxP-Neo-loxP in the intron. The 'FLLFLNL' version (loxP-Neo-loxP) is also available from Addgene.
Proper citation: RRID:Addgene_36908 Copy
Species: Homo sapiens
Genetic Insert: PBGD fragment
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3931; Vector Backbone:pCR2.1; Vector Types:; Bacterial Resistance:Ampicillin
Comments: PBGD fragment is located at bp# 294-366
Proper citation: RRID:Addgene_36869 Copy
Species: Homo sapiens
Genetic Insert: RFXANK
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:7325; Vector Backbone:pET28-MHL (Addgene plasmid 26096); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: PDB: 3UXG. http://www.thesgc.org/structures/3UXG/
Proper citation: RRID:Addgene_36902 Copy
Species: Homo sapiens
Genetic Insert: ANKRA2
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:7325; Vector Backbone:pET28-MHL (Addgene plasmid 26096); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: PDB: 3V2O. http://www.thesgc.org/structures/3V2O/
PDB: 3V2X http://www.thesgc.org/structures/3V2X/
PDB: 3V31 http://www.thesgc.org/structures/3V31/
Proper citation: RRID:Addgene_36904 Copy
Species: Homo sapiens
Genetic Insert: RFXANK
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:7325; Vector Backbone:pET28-MHL (Addgene plasmid 26096); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: PDB: 3V3O. http://www.thesgc.org/structures/3V3O/
Proper citation: RRID:Addgene_36905 Copy
Species: Homo sapiens
Genetic Insert: CCND1-TALEN_Right
Vector Backbone Description: Backbone Marker:Joung lab (Addgene Plasmid # 32288); Backbone Size:6447; Vector Backbone:JDS74; Vector Types:Mammalian, T7 for in vitro mRNA synthesis; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22484455
Comments: Target binding site: TCGGGGTACGCGCGGCGG
It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.2kb and 5.9kb.
Proper citation: RRID:Addgene_36854 Copy
Species: Ciona intestinalis
Genetic Insert: fluorescent protein voltage sensor
Vector Backbone Description: Backbone Size:5694; Vector Backbone:pCS2+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22958819
Comments: http://www.fluorogenetic-voltage-sensors.org/
This construct is composed of the S1-4 domain of the CiVSP voltage sensor. Super ecliptic pHluorin is inserted at position S249 in the CiVSP sequence.
Proper citation: RRID:Addgene_36855 Copy
Species: Ciona intestinalis
Genetic Insert: fluorescent protein voltage sensor
Vector Backbone Description: Backbone Size:5694; Vector Backbone:pCS2+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22958819
Comments: http://www.fluorogenetic-voltage-sensors.org/
This construct is composed of the S1-4 domain of the CiVSP voltage sensor. Super ecliptic pHluorin is inserted at position A242 in the CiVSP sequence.
Proper citation: RRID:Addgene_36857 Copy
Species: Homo sapiens
Genetic Insert: SIRT6-TALEN_Right
Vector Backbone Description: Backbone Marker:Joung lab (Addgene Plasmid # 32288); Backbone Size:6447; Vector Backbone:JDS74; Vector Types:Mammalian, T7 for in vitro mRNA synthesis; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22484455
Comments: Target binding site: TCCGGGAGGCCGCACTTG
It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.2kb and 5.9kb.
Proper citation: RRID:Addgene_36844 Copy
Species: Homo sapiens
Genetic Insert: WTX T289A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:6100; Vector Backbone:pcDNA3.1 (with Venus added); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22215675
Proper citation: RRID:Addgene_36965 Copy
Species: Homo sapiens
Genetic Insert: VHL-TALEN_Left
Vector Backbone Description: Backbone Marker:Joung lab (Addgene Plasmid # 32285); Backbone Size:6447; Vector Backbone:JDS70; Vector Types:Mammalian, T7 for in vitro mRNA synthesis; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22484455
Comments: Target binding site: TCTGGATCGCGGAGGGAA
It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.2kb and 5.9kb.
Proper citation: RRID:Addgene_36845 Copy
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