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Species: Homo sapiens
Genetic Insert: Mitogen-activated protein kinase phosphatase 7
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pET21a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22547814
Proper citation: RRID:Addgene_37793 Copy
Species: Homo sapiens
Genetic Insert: NUMB isoform 2
Vector Backbone Description: Backbone Marker:Beyer et al., J Virol 2002; Backbone Size:7309; Vector Backbone:M107; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21939656
Proper citation: RRID:Addgene_37803 Copy
Species: Homo sapiens
Genetic Insert: NUMB isoform 4
Vector Backbone Description: Backbone Marker:Beyer et al., J Virol 2002; Backbone Size:7309; Vector Backbone:M107; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21939656
Proper citation: RRID:Addgene_37805 Copy
Species: Homo sapiens
Genetic Insert: BAI3
Vector Backbone Description: Backbone Marker:BD Biosciences; Backbone Size:9761; Vector Backbone:pAcGP67A; Vector Types:Baculovirus Transfer Vectors; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22333914
Proper citation: RRID:Addgene_37848 Copy
Species: Homo sapiens
Genetic Insert: EB1
Vector Backbone Description: Vector Backbone:pShuttle; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22483942
Proper citation: RRID:Addgene_37829 Copy
Species: Homo sapiens
Genetic Insert: EB1
Vector Backbone Description: Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22483942
Comments: This construct is resistant to shRNA #3.
EB1 was inserted into pEGFP-N1 via the SacI/SmaI restriction sites. The second EGFP, was then inserted via the SmaI/AgeI sites.
Proper citation: RRID:Addgene_37925 Copy
Species: Homo sapiens
Genetic Insert: EB1 shRNA #3
Vector Backbone Description: Backbone Marker:Addgene plasmid 8453; Backbone Size:7032; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22483942
Proper citation: RRID:Addgene_37927 Copy
Species: Homo sapiens
Genetic Insert: EB1 shRNA #1
Vector Backbone Description: Backbone Marker:Addgene plasmid 8453; Backbone Size:7032; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22483942
Proper citation: RRID:Addgene_37928 Copy
Species: Homo sapiens
Genetic Insert: Nucleoporin 98
Vector Backbone Description: Backbone Size:2968; Vector Backbone:pPEP-TEV; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21685911
Comments: see also:
Strelkov, S. V., Kreplak, L., Herrmann, H. & Aebi, U. (2004). Methods Cell Biol.78, 25–43.
Proper citation: RRID:Addgene_38037 Copy
Species: Homo sapiens
Genetic Insert: shREV3-5
Vector Backbone Description: Backbone Marker:oligoengine; Backbone Size:4354; Vector Backbone:pSUPERIOR.puro; Vector Types:RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22028621
Proper citation: RRID:Addgene_38030 Copy
Species: Homo sapiens
Genetic Insert: shREV3-4
Vector Backbone Description: Backbone Marker:Trono Laboratory; Backbone Size:11085; Vector Backbone:pLVTHM; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22028621
Comments: A versatile tool for conditional gene expression and knockdown.
Szulc J, Wiznerowicz M, Sauvain MO, Trono D, Aebischer P.
Nat Methods. 2006 Feb;3(2):109-16.
PMID:16432520
Proper citation: RRID:Addgene_38033 Copy
Species: Homo sapiens
Genetic Insert: E2F3a
Vector Backbone Description: Backbone Marker:Vogelstein Lab ; Backbone Size:6600; Vector Backbone:pCMV-Neo-Bam; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8246996
Comments: E2Fs bind DNA as a heterodimer with members of the DP family of transcription factors.
The 5' end of the coding sequence can be difficult to sequence.
Proper citation: RRID:Addgene_37970 Copy
Species: Homo sapiens
Genetic Insert: E2F6
Vector Backbone Description: Backbone Marker:Vogelstein Lab ; Backbone Size:6600; Vector Backbone:pCMV-Neo-Bam; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11171983
Comments: The backbone plasmid pCMV-Neo-Bam is Addgene#16440
E2Fs bind DNA as a heterodimer with members of the DP family of transcription factors.
Proper citation: RRID:Addgene_37965 Copy
Species: Homo sapiens
Genetic Insert: DP1
Vector Backbone Description: Backbone Marker:Vogelstein Lab ; Backbone Size:6600; Vector Backbone:pCMV-Neo-Bam; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8657117
Comments: DP1 functions as a heterodimer with several members of the E2F family of transcription factors.
Proper citation: RRID:Addgene_37968 Copy
Species: Homo sapiens
Genetic Insert: FKBP1A
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pmRFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22722250
Comments: Compared to GenBank reference sequence NM_019892, the INPP5E insert in this plasmid contains a C641A mutation. Please see the full plasmid sequence for the most accurate information.
Proper citation: RRID:Addgene_38001 Copy
Species: Homo sapiens
Genetic Insert: YTHDF2 YTH domain family, member 2
Vector Backbone Description: Backbone Size:5496; Vector Backbone:pFRT/TO/HIS/FLAG/HA-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22681889
Proper citation: RRID:Addgene_38089 Copy
Species: Homo sapiens
Genetic Insert: PES1 pescadillo ribosomal biogenesis factor 1
Vector Backbone Description: Backbone Size:5490; Vector Backbone:pFRT/TO/HIS/FLAG/HA-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22681889
Proper citation: RRID:Addgene_38085 Copy
Species: Homo sapiens
Genetic Insert: PRDX1 peroxiredoxin 1
Vector Backbone Description: Backbone Size:5321; Vector Backbone:pFRT/TO/HIS/FLAG/HA-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22681889
Proper citation: RRID:Addgene_38086 Copy
Species: Homo sapiens
Genetic Insert: FKBP1A
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pmRFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22722250
Comments: Compared to GenBank reference sequence NM_019892, the INPP5E insert in this plasmid contains a C641A mutation. Please see the full plasmid sequence for the most accurate information.
Proper citation: RRID:Addgene_38000 Copy
Species: Homo sapiens
Genetic Insert: EDF1 endothelial differentiation-related factor 1
Vector Backbone Description: Backbone Size:5496; Vector Backbone:pFRT/TO/HIS/FLAG/HA-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22681889
Proper citation: RRID:Addgene_38079 Copy
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