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http://www.addgene.org/35218

Species: Danio rerio
Genetic Insert: Zinc finger array targeting psen2
Vector Backbone Description: Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18657511
Comments: This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene psen2. Please note that this plasmid does NOT contain the psen2 sequence. Users must order the complementary plasmid psen2_L (OZ577) [Addgene plasmid 35217] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290/292 (Addgene plasmids 21872 & 21873), pMLM800/802 (Addgene plasmids 27202 & 27203). The difference between pMLM800/802 and pMLM290/292 is the length of the “spacer” sequence in the full ZFN target site. If the spacer is 7 bp, scientists should use pMLM800/802. If the spacer is 5 or 6 bps, scientists should use pMLM290/292. pMLM290/292 is identical to pST1374 except that it harbors two mutations in the FokI nuclease domain (Q486E, I499L; aka the “-“ mutation see Miller et al., Nat. Biotech 2007, PMID 17603475) which confers heterodimeric behavior on these domains This zinc finger array was tested for binding activity to the sequence 5'-GAGGCGGAC-3' in a bacterial two hybrid assay, and resulted in 21.8 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082) "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934)

Proper citation: RRID:Addgene_35218 Copy   

•   Source: Addgene

http://www.addgene.org/35217

Species: Danio rerio
Genetic Insert: Zinc finger array targeting psen2
Vector Backbone Description: Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18657511
Comments: This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene psen2. Please note that this plasmid does NOT contain the psen2 sequence. Users must order the complementary plasmid psen2_R (OZ578) [Addgene plasmid 35218] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290/292 (Addgene plasmids 21872 & 21873), pMLM800/802 (Addgene plasmids 27202 & 27203). The difference between pMLM800/802 and pMLM290/292 is the length of the “spacer” sequence in the full ZFN target site. If the spacer is 7 bp, scientists should use pMLM800/802. If the spacer is 5 or 6 bps, scientists should use pMLM290/292. pMLM290/292 is identical to pST1374 except that it harbors two mutations in the FokI nuclease domain (Q486E, I499L; aka the “-“ mutation see Miller et al., Nat. Biotech 2007, PMID 17603475) which confers heterodimeric behavior on these domains This zinc finger array was tested for binding activity to the sequence 5'-GGAGATGCC-3' in a bacterial two hybrid assay, and resulted in 3.8 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082) "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934)

Proper citation: RRID:Addgene_35217 Copy   

•   Source: Addgene

http://www.addgene.org/35215

Species: Danio rerio
Genetic Insert: Zinc finger array targeting morc3b
Vector Backbone Description: Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18657511
Comments: This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene morc3b. Please note that this plasmid does NOT contain the morc3b sequence. Users must order the complementary plasmid morc3b_R (OZ576) [Addgene plasmid 35216] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290/292 (Addgene plasmids 21872 & 21873), pMLM800/802 (Addgene plasmids 27202 & 27203). The difference between pMLM800/802 and pMLM290/292 is the length of the “spacer” sequence in the full ZFN target site. If the spacer is 7 bp, scientists should use pMLM800/802. If the spacer is 5 or 6 bps, scientists should use pMLM290/292. pMLM290/292 is identical to pST1374 except that it harbors two mutations in the FokI nuclease domain (Q486E, I499L; aka the “-“ mutation see Miller et al., Nat. Biotech 2007, PMID 17603475) which confers heterodimeric behavior on these domains This zinc finger array was tested for binding activity to the sequence 5'-GCTGAGGTT-3' in a bacterial two hybrid assay, and resulted in 3.13 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082) "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934)

Proper citation: RRID:Addgene_35215 Copy   

•   Source: Addgene

http://www.addgene.org/35685

Species: Danio rerio
Genetic Insert: Kdrl-3p-FokI-KK
Vector Backbone Description: Backbone Size:5057; Vector Backbone:pCS2; Vector Types:mRNA production; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21937602

Proper citation: RRID:Addgene_35685 Copy   

•   Source: Addgene

http://www.addgene.org/35676

Species: Danio rerio
Genetic Insert: Ncad
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21115806

Proper citation: RRID:Addgene_35676 Copy   

•   Source: Addgene

http://www.addgene.org/146147

Species: Danio rerio
Genetic Insert: tubgenomic-PTC90
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19414594
Comments: genomic DNA extracted from NCBI Sequence: NW_003335288.1 Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.

Proper citation: RRID:Addgene_146147 Copy   

•   Source: Addgene

http://www.addgene.org/146279

Species: Danio rerio
Genetic Insert: DrSmg6
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19414594
Comments: Sequence based on parts of the sequence given by NCBI: XM_691326.1 (1073....1994) Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.

Proper citation: RRID:Addgene_146279 Copy   

•   Source: Addgene

http://www.addgene.org/146280

Species: Danio rerio
Genetic Insert: DrSmg7
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19414594
Comments: Sequence based on parts of the sequence given by NCBI: NM_001045083.2 (2259.....2784) Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.

Proper citation: RRID:Addgene_146280 Copy   

•   Source: Addgene

http://www.addgene.org/146284

Species: Danio rerio
Genetic Insert: DrUPF3b
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19414594
Comments: Sequence based on parts of the sequence given by NCBI: NM_200954.1 (769......1815) Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.

Proper citation: RRID:Addgene_146284 Copy   

•   Source: Addgene

http://www.addgene.org/146282

Species: Danio rerio
Genetic Insert: DrUPF2
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19414594
Comments: Sequence based on parts of the sequence given by NCBI: XM_692368.5 Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.

Proper citation: RRID:Addgene_146282 Copy   

•   Source: Addgene

http://www.addgene.org/201905

Species: Danio rerio
Genetic Insert: acvr1l
Vector Backbone Description: Backbone Size:4163; Vector Backbone:pCS2+; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33827919

Proper citation: RRID:Addgene_201905 Copy   

•   Source: Addgene

http://www.addgene.org/201904

Species: Danio rerio
Genetic Insert: acvr1l
Vector Backbone Description: Backbone Size:4120; Vector Backbone:pCS2+ or pCS2; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33827919

Proper citation: RRID:Addgene_201904 Copy   

•   Source: Addgene

http://www.addgene.org/172518

Species: Danio rerio
Genetic Insert: ubiquitin promoter, TagBFP, loxP sites (inversion)
Vector Backbone Description: Vector Backbone:p5E; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:39831309
Comments: Please visit https://www.biorxiv.org/content/10.1101/2024.02.22.581357v1 for bioRxiv preprint.

Proper citation: RRID:Addgene_172518 Copy   

•   Source: Addgene

http://www.addgene.org/194291

Species: Danio rerio
Genetic Insert: cavin1a
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDONRp2R-p3; Vector Types:Multisite gateway entry vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36858041
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.06.09.447684v2 for bioRxiv preprint.

Proper citation: RRID:Addgene_194291 Copy   

•   Source: Addgene

http://www.addgene.org/194294

Species: Danio rerio
Genetic Insert: cavin1a
Vector Backbone Description: Vector Backbone:pT3TS-Dest_R1-R3 (Addgene 140878); Vector Types:In vitro transcription of mRNA; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36858041
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.06.09.447684v2 for bioRxiv preprint.

Proper citation: RRID:Addgene_194294 Copy   

•   Source: Addgene

http://www.addgene.org/195953

Species: Danio rerio
Genetic Insert: exorh:mCherry
Vector Backbone Description: Vector Backbone:pENTR5'; Vector Types:Other; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36975217
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.12.13.520107v1.article-info for bioRxiv preprint.

Proper citation: RRID:Addgene_195953 Copy   

•   Source: Addgene

http://www.addgene.org/168241

Species: Danio rerio
Genetic Insert: rac2 sgRNAs
Vector Backbone Description: Vector Backbone:R4R3 pDest; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33722979

Proper citation: RRID:Addgene_168241 Copy   

•   Source: Addgene

http://www.addgene.org/195979

Species: Danio rerio
Genetic Insert: ubi:mCerulean
Vector Backbone Description: Vector Backbone:pDestTol2pA2; Vector Types:Other; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36975217
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.12.13.520107v1.article-info for bioRxiv preprint.

Proper citation: RRID:Addgene_195979 Copy   

•   Source: Addgene

http://www.addgene.org/191428

Species: Danio rerio
Genetic Insert: pth2 regulatory region
Vector Backbone Description: Backbone Size:4777; Vector Backbone:pDONR-P4-P1R; Vector Types:Tol2 Gateway 5' entry vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36161311
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.05.06.490781v3 for bioRxiv preprint.

Proper citation: RRID:Addgene_191428 Copy   

•   Source: Addgene

http://www.addgene.org/195982

Species: Danio rerio
Genetic Insert: desmin a regulatory elements, mouse beta-globin minimal promoter mApple, exorh:EGFP
Vector Backbone Description: Vector Backbone:pDestTol2pA2; Vector Types:Other; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36975217
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.12.13.520107v1.article-info for bioRxiv preprint.

Proper citation: RRID:Addgene_195982 Copy   

•   Source: Addgene