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Species: Arabidopsis thaliana
Genetic Insert: CRY2 PHR-VHH(GFP)
Vector Backbone Description: Vector Backbone:pHRW; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30759894
Proper citation: RRID:Addgene_126022 Copy
Species: Arabidopsis thaliana
Genetic Insert: CIBN-mCerulean-MP; CRY2 PHR-VHH(GFP)
Vector Backbone Description: Vector Backbone:pHW; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30759894
Proper citation: RRID:Addgene_126025 Copy
Species: Arabidopsis thaliana
Genetic Insert: CRY2 PHRolig-VHH(GFP)
Vector Backbone Description: Vector Backbone:pHRW; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30759894
Proper citation: RRID:Addgene_126023 Copy
Species: Arabidopsis thaliana
Genetic Insert: CIBN-MP; CRY2 PHR-VHH(GFP)
Vector Backbone Description: Vector Backbone:pHW; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30759894
Proper citation: RRID:Addgene_126027 Copy
Species: Arabidopsis thaliana
Genetic Insert: CPK32
Vector Backbone Description: Backbone Marker:NEB; Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28489820
Proper citation: RRID:Addgene_107432 Copy
Species: Arabidopsis thaliana
Genetic Insert: CPK32
Vector Backbone Description: Backbone Marker:NEB; Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28489820
Comments: Addgene NGS results confirmed that this plasmid contains amino acids 1-326 of the CPK32 translation
Proper citation: RRID:Addgene_107431 Copy
Species: Arabidopsis thaliana
Genetic Insert: CPK30
Vector Backbone Description: Backbone Marker:NEB; Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28489820
Proper citation: RRID:Addgene_107430 Copy
Species: Arabidopsis thaliana
Genetic Insert: CPK10
Vector Backbone Description: Backbone Marker:NEB; Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28489820
Comments: Addgene NGS results confirmed that this plasmid contains amino acids 1-326 of the CPK10 translation
Proper citation: RRID:Addgene_107427 Copy
Species: Arabidopsis thaliana
Genetic Insert: Ath-HEN1
Vector Backbone Description: Backbone Size:7517; Vector Backbone:pCFJ350-ttTi5605; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29481550
Proper citation: RRID:Addgene_108362 Copy
Species: Arabidopsis thaliana
Genetic Insert: HDT1
Vector Backbone Description: Vector Backbone:pK7GWIWG2; Vector Types:Plant Expression, RNAi; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:28855334
Comments: Full sequence is not available for this plasmid. Please see the diagnostic digest and partial sequencing results as references for verification
To build the pRCH1:HDT1HDT2 RNAi construct (hdt1,2i), the CaMV 35S promoter in pK7GWIWG2 vector (Limpens et al., 2005) was replaced with RCH1 promoter [pK7GWIWG2(II)]. The RCH1 promoter was first amplified on genomic DNA and cloned into pENTR-D-TOPO. Then it was cut out from the vector by HindIII (partial digestion) and XbaI and recombined with two fragments of the pK7GWIWG2(II) vector in a three-point ligation. The two fragments of pK7GWIWG2(II) vector were obtained by digestion either with HindII and NcoI, or with SpeI (compatible with XbaI) and HindIII. The whole RNAi cassette including the RCH1 promoter was cut out from the vector using ApaI and HindIII and ligated into the pBnRGW binary vector digested with the same enzymes and in such way creating pBnRRGWIWG vector. The RNAi target sequences of HDT1 and HDT2 coding sequences (0.6 kb for each) were combined in one amplicon using a two-step PCR, following the procedure described by Franssen et al. (2015). In brief, the first step PCRs introduced short overlaps (15 bp) in PCR fragments of HDT1 and HDT2 coding sequences with HDT1rnai-F and HDT1rnai-R primers, or with HDT2rnai-F and HDT2rnai-R primers. These two fragments were used as templates in the second-step PCR with HDT1rnai-F and HDT2rnai-R primers. The final PCR fragment was introduced into pENTR-D-TOPO vector and recombined in inverse-repeat orientation into the pBnRRGWIWG binary vector by a LR Gateway reaction (Invitrogen).
Proper citation: RRID:Addgene_108446 Copy
Species: Arabidopsis thaliana
Genetic Insert: HDT1
Vector Backbone Description: Vector Backbone:pBnRGW; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:28855334
Proper citation: RRID:Addgene_108565 Copy
Species: Arabidopsis thaliana
Genetic Insert: TRANSPORT INHIBITOR RESPONSE 1 fused with GUS and NOS terminator
Vector Backbone Description: Backbone Size:2700; Vector Backbone:pAN19; Vector Types:Cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29355850
Proper citation: RRID:Addgene_108547 Copy
Species: Arabidopsis thaliana
Genetic Insert: BPC4
Vector Backbone Description: Vector Backbone:pENTR; Vector Types:Other; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28400782
Comments: Our work is based on genomic approaches in Arabidopsis thaliana and groups of genes are presented in all of the following publications:
Pavicic Mirko (2019). Multifaceted roles of RING-type ubiquitin E3 ligases: reverse phenomic approaches. http://urn.fi/URN:ISBN:978-951-51-5483-5
Pavicic M., Mouhu K., Wang F., Bilicka M., Chovancek E., & Himanen K. (2017). Genomic and phenomic screens for flower related RING type ubiquitin E3 ligases in Arabidopsis. Frontiers in Plant Science. doi.org/10.3389/fpls.2017.00416
Pavicic M., Wang F., Mouhu K., Himanen K. (2019). High throughput in vitro seed germination screen identified new ABA responsive RING-type ubiquitin E3 ligases in Arabidopsis thaliana. Plant Cell Tissue and Organ Culture. https://link.springer.com/article/10.1007/s11240-019-01700-9
Proper citation: RRID:Addgene_118686 Copy
Species: Arabidopsis thaliana
Genetic Insert: AT3G07200
Vector Backbone Description: Vector Backbone:pER8 cHA; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:28400782
Comments: 28 C in GV3101 (pMP90) strain
Our work is based on genomic approaches in Arabidopsis thaliana and groups of genes are presented in all of the following publications:
Pavicic Mirko (2019). Multifaceted roles of RING-type ubiquitin E3 ligases: reverse phenomic approaches. http://urn.fi/URN:ISBN:978-951-51-5483-5
Pavicic M., Mouhu K., Wang F., Bilicka M., Chovancek E., & Himanen K. (2017). Genomic and phenomic screens for flower related RING type ubiquitin E3 ligases in Arabidopsis. Frontiers in Plant Science. doi.org/10.3389/fpls.2017.00416
Pavicic M., Wang F., Mouhu K., Himanen K. (2019). High throughput in vitro seed germination screen identified new ABA responsive RING-type ubiquitin E3 ligases in Arabidopsis thaliana. Plant Cell Tissue and Organ Culture. https://link.springer.com/article/10.1007/s11240-019-01700-9
Proper citation: RRID:Addgene_118689 Copy
Species: Arabidopsis thaliana
Genetic Insert: PHL2
Vector Backbone Description: Vector Backbone:pDONR 221; Vector Types:Other; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28400782
Comments: Our work is based on genomic approaches in Arabidopsis thaliana and groups of genes are presented in all of the following publications:
Pavicic Mirko (2019). Multifaceted roles of RING-type ubiquitin E3 ligases: reverse phenomic approaches. http://urn.fi/URN:ISBN:978-951-51-5483-5
Pavicic M., Mouhu K., Wang F., Bilicka M., Chovancek E., & Himanen K. (2017). Genomic and phenomic screens for flower related RING type ubiquitin E3 ligases in Arabidopsis. Frontiers in Plant Science. doi.org/10.3389/fpls.2017.00416
Pavicic M., Wang F., Mouhu K., Himanen K. (2019). High throughput in vitro seed germination screen identified new ABA responsive RING-type ubiquitin E3 ligases in Arabidopsis thaliana. Plant Cell Tissue and Organ Culture. https://link.springer.com/article/10.1007/s11240-019-01700-9
Proper citation: RRID:Addgene_118683 Copy
Species: Arabidopsis thaliana
Genetic Insert: XERICO
Vector Backbone Description: Vector Backbone:pDONR 221; Vector Types:Other; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28400782
Comments: Our work is based on genomic approaches in Arabidopsis thaliana and groups of genes are presented in all of the following publications:
Pavicic Mirko (2019). Multifaceted roles of RING-type ubiquitin E3 ligases: reverse phenomic approaches. http://urn.fi/URN:ISBN:978-951-51-5483-5
Pavicic M., Mouhu K., Wang F., Bilicka M., Chovancek E., & Himanen K. (2017). Genomic and phenomic screens for flower related RING type ubiquitin E3 ligases in Arabidopsis. Frontiers in Plant Science. doi.org/10.3389/fpls.2017.00416
Pavicic M., Wang F., Mouhu K., Himanen K. (2019). High throughput in vitro seed germination screen identified new ABA responsive RING-type ubiquitin E3 ligases in Arabidopsis thaliana. Plant Cell Tissue and Organ Culture. https://link.springer.com/article/10.1007/s11240-019-01700-9
Proper citation: RRID:Addgene_118684 Copy
Species: Arabidopsis thaliana
Genetic Insert: XERICO
Vector Backbone Description: Vector Backbone:pER8 cHA; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:28400782
Comments: 28 C in GV3101 (pMP90) strain
Our work is based on genomic approaches in Arabidopsis thaliana and groups of genes are presented in all of the following publications:
Pavicic Mirko (2019). Multifaceted roles of RING-type ubiquitin E3 ligases: reverse phenomic approaches. http://urn.fi/URN:ISBN:978-951-51-5483-5
Pavicic M., Mouhu K., Wang F., Bilicka M., Chovancek E., & Himanen K. (2017). Genomic and phenomic screens for flower related RING type ubiquitin E3 ligases in Arabidopsis. Frontiers in Plant Science. doi.org/10.3389/fpls.2017.00416
Pavicic M., Wang F., Mouhu K., Himanen K. (2019). High throughput in vitro seed germination screen identified new ABA responsive RING-type ubiquitin E3 ligases in Arabidopsis thaliana. Plant Cell Tissue and Organ Culture. https://link.springer.com/article/10.1007/s11240-019-01700-9
Proper citation: RRID:Addgene_118690 Copy
Species: Arabidopsis thaliana
Genetic Insert: PHL2
Vector Backbone Description: Vector Backbone:pDEST 566; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28400782
Comments: Our work is based on genomic approaches in Arabidopsis thaliana and groups of genes are presented in all of the following publications:
Pavicic Mirko (2019). Multifaceted roles of RING-type ubiquitin E3 ligases: reverse phenomic approaches. http://urn.fi/URN:ISBN:978-951-51-5483-5
Pavicic M., Mouhu K., Wang F., Bilicka M., Chovancek E., & Himanen K. (2017). Genomic and phenomic screens for flower related RING type ubiquitin E3 ligases in Arabidopsis. Frontiers in Plant Science. doi.org/10.3389/fpls.2017.00416
Pavicic M., Wang F., Mouhu K., Himanen K. (2019). High throughput in vitro seed germination screen identified new ABA responsive RING-type ubiquitin E3 ligases in Arabidopsis thaliana. Plant Cell Tissue and Organ Culture. https://link.springer.com/article/10.1007/s11240-019-01700-9
Proper citation: RRID:Addgene_118676 Copy
Species: Arabidopsis thaliana
Genetic Insert: PHL2
Vector Backbone Description: Vector Backbone:pDEST 544; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28400782
Comments: Our work is based on genomic approaches in Arabidopsis thaliana and groups of genes are presented in all of the following publications:
Pavicic Mirko (2019). Multifaceted roles of RING-type ubiquitin E3 ligases: reverse phenomic approaches. http://urn.fi/URN:ISBN:978-951-51-5483-5
Pavicic M., Mouhu K., Wang F., Bilicka M., Chovancek E., & Himanen K. (2017). Genomic and phenomic screens for flower related RING type ubiquitin E3 ligases in Arabidopsis. Frontiers in Plant Science. doi.org/10.3389/fpls.2017.00416
Pavicic M., Wang F., Mouhu K., Himanen K. (2019). High throughput in vitro seed germination screen identified new ABA responsive RING-type ubiquitin E3 ligases in Arabidopsis thaliana. Plant Cell Tissue and Organ Culture. https://link.springer.com/article/10.1007/s11240-019-01700-9
Proper citation: RRID:Addgene_118672 Copy
Species: Arabidopsis thaliana
Genetic Insert: XERICO
Vector Backbone Description: Vector Backbone:pDEST 544; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28400782
Comments: Our work is based on genomic approaches in Arabidopsis thaliana and groups of genes are presented in all of the following publications:
Pavicic Mirko (2019). Multifaceted roles of RING-type ubiquitin E3 ligases: reverse phenomic approaches. http://urn.fi/URN:ISBN:978-951-51-5483-5
Pavicic M., Mouhu K., Wang F., Bilicka M., Chovancek E., & Himanen K. (2017). Genomic and phenomic screens for flower related RING type ubiquitin E3 ligases in Arabidopsis. Frontiers in Plant Science. doi.org/10.3389/fpls.2017.00416
Pavicic M., Wang F., Mouhu K., Himanen K. (2019). High throughput in vitro seed germination screen identified new ABA responsive RING-type ubiquitin E3 ligases in Arabidopsis thaliana. Plant Cell Tissue and Organ Culture. https://link.springer.com/article/10.1007/s11240-019-01700-9
Proper citation: RRID:Addgene_118673 Copy
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