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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pHRW-CRY2-VHH(GFP)
 
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RRID:Addgene_126022 CRY2 PHR-VHH(GFP) Arabidopsis thaliana Ampicillin PMID:30759894 Vector Backbone:pHRW; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin WT 2026-08-15 01:04:21 0
pHW-CIBN-mCerulean-MP-HRW-CRY2-VHH(GFP)
 
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RRID:Addgene_126025 CIBN-mCerulean-MP; CRY2 PHR-VHH(GFP) Arabidopsis thaliana Ampicillin PMID:30759894 Vector Backbone:pHW; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin WT 2026-08-15 01:04:21 0
pHRW-CRY2olig-VHH(GFP)
 
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RRID:Addgene_126023 CRY2 PHRolig-VHH(GFP) Arabidopsis thaliana Ampicillin PMID:30759894 Vector Backbone:pHRW; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin CRY2 E490G 2026-08-15 01:04:21 0
pHW-CIBN-MP-HFW-CRY2-VHH(GFP)
 
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RRID:Addgene_126027 CIBN-MP; CRY2 PHR-VHH(GFP) Arabidopsis thaliana Ampicillin PMID:30759894 Vector Backbone:pHW; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin WT 2026-08-15 01:04:21 0
HBT-CPK32-Flag
 
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RRID:Addgene_107432 CPK32 Arabidopsis thaliana Ampicillin PMID:28489820 Backbone Marker:NEB; Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:01:10 0
HBT-CPK32ac-Flag
 
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RRID:Addgene_107431 CPK32 Arabidopsis thaliana Ampicillin PMID:28489820 Addgene NGS results confirmed that this plasmid contains amino acids 1-326 of the CPK32 translation Backbone Marker:NEB; Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:01:10 0
HBT-CPK30-Flag
 
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RRID:Addgene_107430 CPK30 Arabidopsis thaliana Ampicillin PMID:28489820 Backbone Marker:NEB; Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:01:10 0
HBT-CPK10ac-Flag
 
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RRID:Addgene_107427 CPK10 Arabidopsis thaliana Ampicillin PMID:28489820 Addgene NGS results confirmed that this plasmid contains amino acids 1-326 of the CPK10 translation Backbone Marker:NEB; Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:01:10 0
Ath-HEN1::T2A::mCherry::TY1::H2B-pCFJ350-ttTi5605
 
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RRID:Addgene_108362 Ath-HEN1 Arabidopsis thaliana Ampicillin PMID:29481550 Backbone Size:7517; Vector Backbone:pCFJ350-ttTi5605; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:01:19 0
RCH1:HDT1,HDT2-RNAi
 
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RRID:Addgene_108446 HDT1 Arabidopsis thaliana Spectinomycin PMID:28855334 Full sequence is not available for this plasmid. Please see the diagnostic digest and partial sequencing results as references for verification To build the pRCH1:HDT1HDT2 RNAi construct (hdt1,2i), the CaMV 35S promoter in pK7GWIWG2 vector (Limpens et al., 2005) was replaced with RCH1 promoter [pK7GWIWG2(II)]. The RCH1 promoter was first amplified on genomic DNA and cloned into pENTR-D-TOPO. Then it was cut out from the vector by HindIII (partial digestion) and XbaI and recombined with two fragments of the pK7GWIWG2(II) vector in a three-point ligation. The two fragments of pK7GWIWG2(II) vector were obtained by digestion either with HindII and NcoI, or with SpeI (compatible with XbaI) and HindIII. The whole RNAi cassette including the RCH1 promoter was cut out from the vector using ApaI and HindIII and ligated into the pBnRGW binary vector digested with the same enzymes and in such way creating pBnRRGWIWG vector. The RNAi target sequences of HDT1 and HDT2 coding sequences (0.6 kb for each) were combined in one amplicon using a two-step PCR, following the procedure described by Franssen et al. (2015). In brief, the first step PCRs introduced short overlaps (15 bp) in PCR fragments of HDT1 and HDT2 coding sequences with HDT1rnai-F and HDT1rnai-R primers, or with HDT2rnai-F and HDT2rnai-R primers. These two fragments were used as templates in the second-step PCR with HDT1rnai-F and HDT2rnai-R primers. The final PCR fragment was introduced into pENTR-D-TOPO vector and recombined in inverse-repeat orientation into the pBnRRGWIWG binary vector by a LR Gateway reaction (Invitrogen). Vector Backbone:pK7GWIWG2; Vector Types:Plant Expression, RNAi; Bacterial Resistance:Spectinomycin 2026-08-15 01:01:20 0
pHDT1:HDT1-GFP
 
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RRID:Addgene_108565 HDT1 Arabidopsis thaliana Spectinomycin PMID:28855334 Vector Backbone:pBnRGW; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin 2026-08-15 01:01:21 0
pAN19-TIR1-GUS-NOSt
 
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RRID:Addgene_108547 TRANSPORT INHIBITOR RESPONSE 1 fused with GUS and NOS terminator Arabidopsis thaliana Ampicillin PMID:29355850 Backbone Size:2700; Vector Backbone:pAN19; Vector Types:Cloning vector; Bacterial Resistance:Ampicillin 2026-08-15 01:01:21 0
pENTR-BPC4 (NON-STOP)
 
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RRID:Addgene_118686 BPC4 Arabidopsis thaliana Kanamycin PMID:28400782 Our work is based on genomic approaches in Arabidopsis thaliana and groups of genes are presented in all of the following publications: Pavicic Mirko (2019). Multifaceted roles of RING-type ubiquitin E3 ligases: reverse phenomic approaches. http://urn.fi/URN:ISBN:978-951-51-5483-5
 Pavicic M., Mouhu K., Wang F., Bilicka M., Chovancek E., & Himanen K. (2017). Genomic and phenomic screens for flower related RING type ubiquitin E3 ligases in Arabidopsis. Frontiers in Plant Science. doi.org/10.3389/fpls.2017.00416
   
 Pavicic M., Wang F., Mouhu K., Himanen K. (2019). High throughput in vitro seed germination screen identified new ABA responsive RING-type ubiquitin E3 ligases in Arabidopsis thaliana. Plant Cell Tissue and Organ Culture. https://link.springer.com/article/10.1007/s11240-019-01700-9 Vector Backbone:pENTR; Vector Types:Other; Bacterial Resistance:Kanamycin 2026-08-15 01:03:07 0
pER8 cHA-FG1
 
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RRID:Addgene_118689 AT3G07200 Arabidopsis thaliana Spectinomycin PMID:28400782 28 C in GV3101 (pMP90) strain Our work is based on genomic approaches in Arabidopsis thaliana and groups of genes are presented in all of the following publications: Pavicic Mirko (2019). Multifaceted roles of RING-type ubiquitin E3 ligases: reverse phenomic approaches. http://urn.fi/URN:ISBN:978-951-51-5483-5
 Pavicic M., Mouhu K., Wang F., Bilicka M., Chovancek E., & Himanen K. (2017). Genomic and phenomic screens for flower related RING type ubiquitin E3 ligases in Arabidopsis. Frontiers in Plant Science. doi.org/10.3389/fpls.2017.00416
   
 Pavicic M., Wang F., Mouhu K., Himanen K. (2019). High throughput in vitro seed germination screen identified new ABA responsive RING-type ubiquitin E3 ligases in Arabidopsis thaliana. Plant Cell Tissue and Organ Culture. https://link.springer.com/article/10.1007/s11240-019-01700-9 Vector Backbone:pER8 cHA; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin 2026-08-15 01:03:06 0
pDONR 221-PHL2 (STOP)
 
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1+ mentions
RRID:Addgene_118683 PHL2 Arabidopsis thaliana Kanamycin PMID:28400782 Our work is based on genomic approaches in Arabidopsis thaliana and groups of genes are presented in all of the following publications: Pavicic Mirko (2019). Multifaceted roles of RING-type ubiquitin E3 ligases: reverse phenomic approaches. http://urn.fi/URN:ISBN:978-951-51-5483-5
 Pavicic M., Mouhu K., Wang F., Bilicka M., Chovancek E., & Himanen K. (2017). Genomic and phenomic screens for flower related RING type ubiquitin E3 ligases in Arabidopsis. Frontiers in Plant Science. doi.org/10.3389/fpls.2017.00416
   
 Pavicic M., Wang F., Mouhu K., Himanen K. (2019). High throughput in vitro seed germination screen identified new ABA responsive RING-type ubiquitin E3 ligases in Arabidopsis thaliana. Plant Cell Tissue and Organ Culture. https://link.springer.com/article/10.1007/s11240-019-01700-9 Vector Backbone:pDONR 221; Vector Types:Other; Bacterial Resistance:Kanamycin 2026-08-15 01:03:07 1
pDONR 221-XERICO (NON-STOP)
 
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RRID:Addgene_118684 XERICO Arabidopsis thaliana Kanamycin PMID:28400782 Our work is based on genomic approaches in Arabidopsis thaliana and groups of genes are presented in all of the following publications: Pavicic Mirko (2019). Multifaceted roles of RING-type ubiquitin E3 ligases: reverse phenomic approaches. http://urn.fi/URN:ISBN:978-951-51-5483-5
 Pavicic M., Mouhu K., Wang F., Bilicka M., Chovancek E., & Himanen K. (2017). Genomic and phenomic screens for flower related RING type ubiquitin E3 ligases in Arabidopsis. Frontiers in Plant Science. doi.org/10.3389/fpls.2017.00416
   
 Pavicic M., Wang F., Mouhu K., Himanen K. (2019). High throughput in vitro seed germination screen identified new ABA responsive RING-type ubiquitin E3 ligases in Arabidopsis thaliana. Plant Cell Tissue and Organ Culture. https://link.springer.com/article/10.1007/s11240-019-01700-9 Vector Backbone:pDONR 221; Vector Types:Other; Bacterial Resistance:Kanamycin 2026-08-15 01:03:06 0
pER8 cHA-XERICO
 
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RRID:Addgene_118690 XERICO Arabidopsis thaliana Spectinomycin PMID:28400782 28 C in GV3101 (pMP90) strain Our work is based on genomic approaches in Arabidopsis thaliana and groups of genes are presented in all of the following publications: Pavicic Mirko (2019). Multifaceted roles of RING-type ubiquitin E3 ligases: reverse phenomic approaches. http://urn.fi/URN:ISBN:978-951-51-5483-5
 Pavicic M., Mouhu K., Wang F., Bilicka M., Chovancek E., & Himanen K. (2017). Genomic and phenomic screens for flower related RING type ubiquitin E3 ligases in Arabidopsis. Frontiers in Plant Science. doi.org/10.3389/fpls.2017.00416
   
 Pavicic M., Wang F., Mouhu K., Himanen K. (2019). High throughput in vitro seed germination screen identified new ABA responsive RING-type ubiquitin E3 ligases in Arabidopsis thaliana. Plant Cell Tissue and Organ Culture. https://link.springer.com/article/10.1007/s11240-019-01700-9 Vector Backbone:pER8 cHA; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin 2026-08-15 01:03:06 0
pDEST 566-PHL2
 
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RRID:Addgene_118676 PHL2 Arabidopsis thaliana Ampicillin PMID:28400782 Our work is based on genomic approaches in Arabidopsis thaliana and groups of genes are presented in all of the following publications: Pavicic Mirko (2019). Multifaceted roles of RING-type ubiquitin E3 ligases: reverse phenomic approaches. http://urn.fi/URN:ISBN:978-951-51-5483-5
 Pavicic M., Mouhu K., Wang F., Bilicka M., Chovancek E., & Himanen K. (2017). Genomic and phenomic screens for flower related RING type ubiquitin E3 ligases in Arabidopsis. Frontiers in Plant Science. doi.org/10.3389/fpls.2017.00416
   
 Pavicic M., Wang F., Mouhu K., Himanen K. (2019). High throughput in vitro seed germination screen identified new ABA responsive RING-type ubiquitin E3 ligases in Arabidopsis thaliana. Plant Cell Tissue and Organ Culture. https://link.springer.com/article/10.1007/s11240-019-01700-9 Vector Backbone:pDEST 566; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:03:06 0
pDEST 544-PHL2
 
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RRID:Addgene_118672 PHL2 Arabidopsis thaliana Ampicillin PMID:28400782 Our work is based on genomic approaches in Arabidopsis thaliana and groups of genes are presented in all of the following publications: Pavicic Mirko (2019). Multifaceted roles of RING-type ubiquitin E3 ligases: reverse phenomic approaches. http://urn.fi/URN:ISBN:978-951-51-5483-5
 Pavicic M., Mouhu K., Wang F., Bilicka M., Chovancek E., & Himanen K. (2017). Genomic and phenomic screens for flower related RING type ubiquitin E3 ligases in Arabidopsis. Frontiers in Plant Science. doi.org/10.3389/fpls.2017.00416
   
 Pavicic M., Wang F., Mouhu K., Himanen K. (2019). High throughput in vitro seed germination screen identified new ABA responsive RING-type ubiquitin E3 ligases in Arabidopsis thaliana. Plant Cell Tissue and Organ Culture. https://link.springer.com/article/10.1007/s11240-019-01700-9 Vector Backbone:pDEST 544; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:03:06 0
pDEST 544-XERICO
 
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RRID:Addgene_118673 XERICO Arabidopsis thaliana Ampicillin PMID:28400782 Our work is based on genomic approaches in Arabidopsis thaliana and groups of genes are presented in all of the following publications: Pavicic Mirko (2019). Multifaceted roles of RING-type ubiquitin E3 ligases: reverse phenomic approaches. http://urn.fi/URN:ISBN:978-951-51-5483-5
 Pavicic M., Mouhu K., Wang F., Bilicka M., Chovancek E., & Himanen K. (2017). Genomic and phenomic screens for flower related RING type ubiquitin E3 ligases in Arabidopsis. Frontiers in Plant Science. doi.org/10.3389/fpls.2017.00416
   
 Pavicic M., Wang F., Mouhu K., Himanen K. (2019). High throughput in vitro seed germination screen identified new ABA responsive RING-type ubiquitin E3 ligases in Arabidopsis thaliana. Plant Cell Tissue and Organ Culture. https://link.springer.com/article/10.1007/s11240-019-01700-9 Vector Backbone:pDEST 544; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:03:06 0

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