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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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psen2_R (OZ578) Resource Report Resource Website |
RRID:Addgene_35218 | Zinc finger array targeting psen2 | Danio rerio | Kanamycin | PMID:18657511 | This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene psen2. Please note that this plasmid does NOT contain the psen2 sequence. Users must order the complementary plasmid psen2_L (OZ577) [Addgene plasmid 35217] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290/292 (Addgene plasmids 21872 & 21873), pMLM800/802 (Addgene plasmids 27202 & 27203). The difference between pMLM800/802 and pMLM290/292 is the length of the “spacer” sequence in the full ZFN target site. If the spacer is 7 bp, scientists should use pMLM800/802. If the spacer is 5 or 6 bps, scientists should use pMLM290/292. pMLM290/292 is identical to pST1374 except that it harbors two mutations in the FokI nuclease domain (Q486E, I499L; aka the “-“ mutation see Miller et al., Nat. Biotech 2007, PMID 17603475) which confers heterodimeric behavior on these domains This zinc finger array was tested for binding activity to the sequence 5'-GAGGCGGAC-3' in a bacterial two hybrid assay, and resulted in 21.8 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082) "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934) | Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin | 2026-08-15 01:13:58 | 0 | |
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psen2_L (OZ577) Resource Report Resource Website |
RRID:Addgene_35217 | Zinc finger array targeting psen2 | Danio rerio | Kanamycin | PMID:18657511 | This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene psen2. Please note that this plasmid does NOT contain the psen2 sequence. Users must order the complementary plasmid psen2_R (OZ578) [Addgene plasmid 35218] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290/292 (Addgene plasmids 21872 & 21873), pMLM800/802 (Addgene plasmids 27202 & 27203). The difference between pMLM800/802 and pMLM290/292 is the length of the “spacer” sequence in the full ZFN target site. If the spacer is 7 bp, scientists should use pMLM800/802. If the spacer is 5 or 6 bps, scientists should use pMLM290/292. pMLM290/292 is identical to pST1374 except that it harbors two mutations in the FokI nuclease domain (Q486E, I499L; aka the “-“ mutation see Miller et al., Nat. Biotech 2007, PMID 17603475) which confers heterodimeric behavior on these domains This zinc finger array was tested for binding activity to the sequence 5'-GGAGATGCC-3' in a bacterial two hybrid assay, and resulted in 3.8 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082) "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934) | Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin | 2026-08-15 01:13:58 | 0 | |
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morc3b_L (OZ575) Resource Report Resource Website |
RRID:Addgene_35215 | Zinc finger array targeting morc3b | Danio rerio | Kanamycin | PMID:18657511 | This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene morc3b. Please note that this plasmid does NOT contain the morc3b sequence. Users must order the complementary plasmid morc3b_R (OZ576) [Addgene plasmid 35216] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290/292 (Addgene plasmids 21872 & 21873), pMLM800/802 (Addgene plasmids 27202 & 27203). The difference between pMLM800/802 and pMLM290/292 is the length of the “spacer” sequence in the full ZFN target site. If the spacer is 7 bp, scientists should use pMLM800/802. If the spacer is 5 or 6 bps, scientists should use pMLM290/292. pMLM290/292 is identical to pST1374 except that it harbors two mutations in the FokI nuclease domain (Q486E, I499L; aka the “-“ mutation see Miller et al., Nat. Biotech 2007, PMID 17603475) which confers heterodimeric behavior on these domains This zinc finger array was tested for binding activity to the sequence 5'-GCTGAGGTT-3' in a bacterial two hybrid assay, and resulted in 3.13 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082) "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934) | Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin | 2026-08-15 01:13:58 | 0 | |
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pCS2-HA-Kdrl-3p-FokI-KK Resource Report Resource Website |
RRID:Addgene_35685 | Kdrl-3p-FokI-KK | Danio rerio | Ampicillin | PMID:21937602 | Backbone Size:5057; Vector Backbone:pCS2; Vector Types:mRNA production; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:03 | 0 | ||
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pEGFP-N1- Ncad-GFP Resource Report Resource Website |
RRID:Addgene_35676 | Ncad | Danio rerio | Kanamycin | PMID:21115806 | Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:14:03 | 0 | ||
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pT7-EGFP-C1-tubgenomic-PTC90_D Resource Report Resource Website |
RRID:Addgene_146147 | tubgenomic-PTC90 | Danio rerio | Kanamycin | PMID:19414594 | genomic DNA extracted from NCBI Sequence: NW_003335288.1 Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid. | Vector Backbone:Unknown; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:25:30 | 0 | |
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pCRII-TOPO-DrSmg6_F Resource Report Resource Website |
RRID:Addgene_146279 | DrSmg6 | Danio rerio | Ampicillin | PMID:19414594 | Sequence based on parts of the sequence given by NCBI: XM_691326.1 (1073....1994) Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid. | Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:25:31 | 0 | |
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pCRII-TOPO-DrSmg7_F Resource Report Resource Website |
RRID:Addgene_146280 | DrSmg7 | Danio rerio | Ampicillin | PMID:19414594 | Sequence based on parts of the sequence given by NCBI: NM_001045083.2 (2259.....2784) Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid. | Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:25:31 | 0 | |
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pCRII-TOPO-DrUPF3b_F Resource Report Resource Website |
RRID:Addgene_146284 | DrUPF3b | Danio rerio | Ampicillin | PMID:19414594 | Sequence based on parts of the sequence given by NCBI: NM_200954.1 (769......1815) Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid. | Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:25:31 | 0 | |
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pCRII-TOPO-DrUPF2_F Resource Report Resource Website |
RRID:Addgene_146282 | DrUPF2 | Danio rerio | Ampicillin | PMID:19414594 | Sequence based on parts of the sequence given by NCBI: XM_692368.5 Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid. | Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:25:31 | 0 | |
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acvr1l-VAAA-HA Resource Report Resource Website |
RRID:Addgene_201905 | acvr1l | Danio rerio | Ampicillin | PMID:33827919 | Backbone Size:4163; Vector Backbone:pCS2+; Vector Types:; Bacterial Resistance:Ampicillin | T186V, S187A, S189A, S191A | 2026-08-15 01:26:36 | 0 | |
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acvr1l-HA Resource Report Resource Website |
RRID:Addgene_201904 | acvr1l | Danio rerio | Ampicillin | PMID:33827919 | Backbone Size:4120; Vector Backbone:pCS2+ or pCS2; Vector Types:; Bacterial Resistance:Ampicillin | 2026-08-15 01:26:36 | 0 | ||
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50_p5E_UBI_loxTagBFP Resource Report Resource Website 1+ mentions |
RRID:Addgene_172518 | ubiquitin promoter, TagBFP, loxP sites (inversion) | Danio rerio | Kanamycin | PMID:39831309 | Please visit https://www.biorxiv.org/content/10.1101/2024.02.22.581357v1 for bioRxiv preprint. | Vector Backbone:p5E; Vector Types:; Bacterial Resistance:Kanamycin | 2026-08-15 01:24:59 | 1 | |
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p3E-DrCavin1a Resource Report Resource Website |
RRID:Addgene_194291 | cavin1a | Danio rerio | Kanamycin | PMID:36858041 | Please visit https://www.biorxiv.org/content/10.1101/2021.06.09.447684v2 for bioRxiv preprint. | Backbone Marker:Invitrogen; Vector Backbone:pDONRp2R-p3; Vector Types:Multisite gateway entry vector; Bacterial Resistance:Kanamycin | 2026-08-15 01:24:35 | 0 | |
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mKate2-DrCavin1a in pT3TS-Dest Resource Report Resource Website |
RRID:Addgene_194294 | cavin1a | Danio rerio | Ampicillin | PMID:36858041 | Please visit https://www.biorxiv.org/content/10.1101/2021.06.09.447684v2 for bioRxiv preprint. | Vector Backbone:pT3TS-Dest_R1-R3 (Addgene 140878); Vector Types:In vitro transcription of mRNA; Bacterial Resistance:Ampicillin | 2026-08-15 01:24:35 | 0 | |
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pCK052 p5E exorh:mCherry Resource Report Resource Website |
RRID:Addgene_195953 | exorh:mCherry | Danio rerio | Kanamycin | PMID:36975217 | Please visit https://www.biorxiv.org/content/10.1101/2022.12.13.520107v1.article-info for bioRxiv preprint. | Vector Backbone:pENTR5'; Vector Types:Other; Bacterial Resistance:Kanamycin | 2026-08-15 01:25:58 | 0 | |
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Tol2-LyzC-GFP-U6ac-rac2-guides Resource Report Resource Website |
RRID:Addgene_168241 | rac2 sgRNAs | Danio rerio | Ampicillin | PMID:33722979 | Vector Backbone:R4R3 pDest; Vector Types:CRISPR; Bacterial Resistance:Ampicillin | 2026-08-15 01:25:03 | 0 | ||
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pCK005 ubi:mCerulean_ubb-polyA Resource Report Resource Website |
RRID:Addgene_195979 | ubi:mCerulean | Danio rerio | Ampicillin | PMID:36975217 | Please visit https://www.biorxiv.org/content/10.1101/2022.12.13.520107v1.article-info for bioRxiv preprint. | Vector Backbone:pDestTol2pA2; Vector Types:Other; Bacterial Resistance:Ampicillin | 2026-08-15 01:25:11 | 0 | |
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p5E-pth2 Resource Report Resource Website |
RRID:Addgene_191428 | pth2 regulatory region | Danio rerio | Kanamycin | PMID:36161311 | Please visit https://www.biorxiv.org/content/10.1101/2022.05.06.490781v3 for bioRxiv preprint. | Backbone Size:4777; Vector Backbone:pDONR-P4-P1R; Vector Types:Tol2 Gateway 5' entry vector; Bacterial Resistance:Kanamycin | 2026-08-15 01:25:11 | 0 | |
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pCK069 desmaMCS:minprom_mApple,exorh:EGFP Resource Report Resource Website |
RRID:Addgene_195982 | desmin a regulatory elements, mouse beta-globin minimal promoter mApple, exorh:EGFP | Danio rerio | Ampicillin | PMID:36975217 | Please visit https://www.biorxiv.org/content/10.1101/2022.12.13.520107v1.article-info for bioRxiv preprint. | Vector Backbone:pDestTol2pA2; Vector Types:Other; Bacterial Resistance:Ampicillin | 2026-08-15 01:25:11 | 0 |
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