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Species: Homo sapiens
Genetic Insert: sgRNA against NFATc1
Vector Backbone Description: Backbone Marker:Feng Zhang (Plasmid #48138); Backbone Size:9703; Vector Backbone:PX458-pSpCas9(BB)2A-GFP; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27637333
Comments: gRNA contains at least 3 missmatches against human DNA (http://crispr.mit.edu). 2 additional nonmatching Guanidines added at 5' to enhance specificity (Kim, D. et al. Digenome-seq: genome-wide profiling of CRISPR-Cas9 off-target effects in human cells. Nat. Methods 12, 237–43, 1 p following 243 (2015).)
targets all isoforms.
Proper citation: RRID:Addgene_75236 Copy
Species: Homo sapiens
Genetic Insert: sgRNA against NFATc2
Vector Backbone Description: Backbone Marker:Feng Zhang (Plasmid #48138); Backbone Size:9703; Vector Backbone:PX458-pSpCas9(BB)2A-GFP; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27637333
Comments: gRNA contains at least 3 missmatches against human DNA (http://crispr.mit.edu). 2 additional nonmatching Guanidines added at 5' to enhnace specificy (Kim, D. et al. Digenome-seq: genome-wide profiling of CRISPR-Cas9 off-target effects in human cells. Nat. Methods 12, 237–43, 1 p following 243 (2015).)
targets all isoforms.
Proper citation: RRID:Addgene_75233 Copy
Species: Homo sapiens
Genetic Insert: sgRNA against NFATc1
Vector Backbone Description: Backbone Marker:Feng Zhang (Plasmid #48140); Backbone Size:9703; Vector Backbone:PX461-pSpCas9n(BB)2A-GFP; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27637333
Comments: gRNA contains at least 3 missmatches against human DNA (http://crispr.mit.edu). 2 additional nonmatching Guanidines added at 5' to enhnace specificy (Kim, D. et al. Digenome-seq: genome-wide profiling of CRISPR-Cas9 off-target effects in human cells. Nat. Methods 12, 237–43, 1 p following 243 (2015).)
targets all isoforms.
MUST BE COTRANSFECTED WITH PLASMID #75238
Proper citation: RRID:Addgene_75239 Copy
Species: Homo sapiens
Genetic Insert: sgRNA against NFATc1
Vector Backbone Description: Backbone Marker:Feng Zhang (Plasmid #48140); Backbone Size:9703; Vector Backbone:PX461-pSpCas9n(BB)2A-GFP; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27637333
Comments: gRNA contains at least 3 missmatches against human DNA (http://crispr.mit.edu). 2 additional nonmatching Guanidines added at 5' to enhnace specificy (Kim, D. et al. Digenome-seq: genome-wide profiling of CRISPR-Cas9 off-target effects in human cells. Nat. Methods 12, 237–43, 1 p following 243 (2015).)
targets all isoforms.
MUST BE COTRANSFECTED WITH PLASMID #75239
Proper citation: RRID:Addgene_75238 Copy
Species: Homo sapiens
Genetic Insert: sgRNA against NFATc1
Vector Backbone Description: Backbone Marker:Feng Zhang (Plasmid #48138); Backbone Size:9703; Vector Backbone:PX458-pSpCas9(BB)2A-GFP; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27637333
Comments: gRNA contains at least 3 missmatches against human DNA (http://crispr.mit.edu). 2 additional nonmatching Guanidines added at 5' to enhance specificity (Kim, D. et al. Digenome-seq: genome-wide profiling of CRISPR-Cas9 off-target effects in human cells. Nat. Methods 12, 237–43, 1 p following 243 (2015).)
targets all isoforms.
Proper citation: RRID:Addgene_75237 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Ret2LD2a N152T
Vector Backbone Description: Vector Backbone:p415; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27298352
Proper citation: RRID:Addgene_75260 Copy
Species: Homo sapiens
Genetic Insert: TTF-1
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22761434
Proper citation: RRID:Addgene_75265 Copy
Species: Homo sapiens
Genetic Insert: Cell Division Cycle 42
Vector Backbone Description: Backbone Size:5040; Vector Backbone:pTriEx; Vector Types:Mammalian Expression, Bacterial Expression, Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19693014
Proper citation: RRID:Addgene_75263 Copy
Species: Homo sapiens
Genetic Insert: Bcl10
Vector Backbone Description: Backbone Size:8400; Vector Backbone:pMSCV-IRES-GFP (pMIG); Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27637333
Comments: EF1a promoter ensures enhanced transgene transcription in some cells, but significantly reduces virus titer. This may result in less transduced cells, which produce high amounts of transgene, though, and can be sorted via GFP.
Proper citation: RRID:Addgene_75256 Copy
Species: Homo sapiens
Genetic Insert: shRNA against MIB2 (human)
Vector Backbone Description: Vector Backbone:pQCXIX (modified); Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27637333
Comments: Produced >90 % KD (Western Blot) in Jurkat cells after RV-transduction and sort for GFPpositive cells.
Proper citation: RRID:Addgene_75255 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Ret2LD2a
Vector Backbone Description: Vector Backbone:p415; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27298352
Proper citation: RRID:Addgene_75259 Copy
Species: Homo sapiens
Genetic Insert: H2bk
Vector Backbone Description: Backbone Marker:Evrogen; Backbone Size:4705; Vector Backbone:pDendra2-N; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24637833
Proper citation: RRID:Addgene_75283 Copy
Species: Homo sapiens
Genetic Insert: KRAS
Vector Backbone Description: Backbone Size:5169; Vector Backbone:pBabe; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_75282 Copy
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:6720; Vector Backbone:pMAL-p2X; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20535468
Proper citation: RRID:Addgene_75287 Copy
Species: Synthetic
Genetic Insert: Sacsin Serinus canaria 90-1368
Vector Backbone Description: Backbone Marker:GE Healthcare ; Backbone Size:4984; Vector Backbone:pGEX6P1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_75321 Copy
Species: Synthetic
Genetic Insert: Sacsin Jatropha-curcas 1-1304
Vector Backbone Description: Backbone Marker:GE Healthcare ; Backbone Size:4984; Vector Backbone:pGEX6P1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_75327 Copy
Species: Synthetic
Genetic Insert: Sacsin Nelumbo nucifera 1-1313
Vector Backbone Description: Backbone Marker:GE Healthcare ; Backbone Size:4984; Vector Backbone:pGEX6P1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_75326 Copy
Species: Synthetic
Genetic Insert: Sacsin Cucumis sativus 1-1292
Vector Backbone Description: Backbone Marker:GE Healthcare ; Backbone Size:4984; Vector Backbone:pGEX6P1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_75325 Copy
Genetic Insert: Arrestin independent version of optoB2
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26412387
Proper citation: RRID:Addgene_75270 Copy
Genetic Insert: 3,052 unique sgRNAs targeting 763 human kinase genes along with 100 non-targeting controls
Vector Backbone Description: Vector Backbone:lentiGuide-Puro; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26780180
Proper citation: RRID:Addgene_75312 Copy
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