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Species: Saccharomyces cerevisiae
Genetic Insert: PI-SceI
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET-23a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7890714
Comments: Expresses the excised intein of the VMA1 gene product including amino-terminal methionine-cysteine residues. Contains silent AvrII and RsrII sites near the borders of the N-terminal LAGLIDADG motif and silent EaeI and SnaBI sites near the borders of the C-terminal motif. Contains a D218A mutation in the first LAGLIDADG motif that inactivates the protein.
Proper citation: RRID:Addgene_195608 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: PI-SceI
Vector Backbone Description: Vector Backbone:pT7-7; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7890714
Comments: Expresses the excised intein of the VMA1 gene product including amino-terminal methionine-cysteine residues. Contains silent AvrII and RsrII sites near the borders of the N-terminal LAGLIDADG motif and silent EaeI and SnaBI sites near the borders of the C-terminal motif. Contains a D326A mutation in the second LAGLIDADG motif that inactivates the protein.
Proper citation: RRID:Addgene_195609 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ACH1
Vector Backbone Description: Vector Backbone:UG72-2GFP; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32859196
Proper citation: RRID:Addgene_184769 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SEC7
Vector Backbone Description: Vector Backbone:UG74-2GFP; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32859196
Proper citation: RRID:Addgene_184764 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SYS1
Vector Backbone Description: Vector Backbone:UG72-2GFP; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32859196
Proper citation: RRID:Addgene_184771 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: proGAL-Aur1
Vector Backbone Description: Vector Backbone:GAL-UG75; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32859196
Proper citation: RRID:Addgene_184775 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: mak10 gRNA
Vector Backbone Description: Backbone Marker:Addgene $ 60847; Backbone Size:8761; Vector Backbone:pCAS; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_160385 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: KanR
Vector Backbone Description: Backbone Size:2400; Vector Backbone:pFA6a; Vector Types:yeast genomic targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36503721
Comments: This plasmid can be used in place of the original pFA6a series to introduce targeted mutation by selectable marker insertion in yeast while preventing off-target disruption of insertion neighboring genes caused by cassette promoter driven transcription interference.
For more information regarding these plasmids see our article (Powers et al., eLife 2022) "Bidirectional promoter activity from expression cassettes can drive off-target repression of neighboring gene translation"
The backbone and KanMX cassette was amplified from pFA6a-kanMX6 (Longtine et al., 1998), the tDEG1 sequence was amplified from S. cerevisiae gDNA.
Proper citation: RRID:Addgene_195038 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: His5+
Vector Backbone Description: Backbone Size:2458; Vector Backbone:pFA6a; Vector Types:yeast genomic targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36503721
Comments: This plasmid can be used in place of the original pFA6a series to introduce targeted mutation by selectable marker insertion in yeast while preventing off-target disruption of insertion neighboring genes caused by cassette promoter driven transcription interference.
For more information regarding these plasmids see our article (Powers et al., eLife 2022) "Bidirectional promoter activity from expression cassettes can drive off-target repression of neighboring gene translation"
The backbone and KanMX cassette was amplified from pFA6a-kanMX6 (Longtine et al., 1998), the tDEG1 and tCYC1 sequences were amplified from S. cerevisiae gDNA.
Proper citation: RRID:Addgene_195040 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: NatR
Vector Backbone Description: Backbone Size:2458; Vector Backbone:pFA6a; Vector Types:yeast genomic targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36503721
Comments: This plasmid can be used in place of the original pFA6a series to introduce targeted mutation by selectable marker insertion in yeast while preventing off-target disruption of insertion neighboring genes caused by cassette promoter driven transcription interference.
For more information regarding these plasmids see our article (Powers et al., eLife 2022) "Bidirectional promoter activity from expression cassettes can drive off-target repression of neighboring gene translation"
The backbone and KanMX cassette was amplified from pFA6a-kanMX6 (Longtine et al., 1998), the tDEG1 and tCYC1 sequences were amplified from S. cerevisiae gDNA.
Proper citation: RRID:Addgene_195042 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: NatR
Vector Backbone Description: Backbone Size:2400; Vector Backbone:pFA6a; Vector Types:yeast genomic targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36503721
Comments: This plasmid can be used in place of the original pFA6a series to introduce targeted mutation by selectable marker insertion in yeast while preventing off-target disruption of insertion neighboring genes caused by cassette promoter driven transcription interference.
For more information regarding these plasmids see our article (Powers et al., eLife 2022) "Bidirectional promoter activity from expression cassettes can drive off-target repression of neighboring gene translation"
The backbone was amplified from pFA6a-kanMX6 (Longtine et al., 1998), the NatR gene and C. glabrata PGK1 promoter and terminator were synthesized on a gene block from IDT, the tCYC1 sequence was amplified from S. cerevisiae gDNA.
Proper citation: RRID:Addgene_195044 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: TIF4631 leader sequence
Vector Backbone Description: Backbone Marker:Academic publication; Backbone Size:6018; Vector Backbone:pRS315; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36481666
Proper citation: RRID:Addgene_194519 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: UGP1::mCherry-AID(71-114)-NatMX
Vector Backbone Description: Backbone Marker:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC55758/; Backbone Size:2531; Vector Backbone:pG23A, originally HO-poly-KanMX4-HO; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36849832
Comments: Cloning details are provided in Supplementary Table 2 of the paper. Details of the parent plasmid pG23A can be found on Addgene (Plasmid #102884).
Proper citation: RRID:Addgene_196615 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ATP3::mCherry-AID(71-114)-NatMX
Vector Backbone Description: Backbone Marker:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC55758/; Backbone Size:2531; Vector Backbone:pG23A, originally HO-poly-KanMX4-HO; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36849832
Comments: Cloning details are provided in Supplementary Table 2 of the paper. Details of the parent plasmid pG23A can be found on Addgene (Plasmid #102884).
Proper citation: RRID:Addgene_196614 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: S000001911
Vector Backbone Description: Vector Backbone:pROS13; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36849832
Comments: Cloning details are in the Methods section and Supplementary Table 2 of the paper.
Proper citation: RRID:Addgene_196613 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Pab1
Vector Backbone Description: Backbone Size:7167; Vector Backbone:pOBD2; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25671604
Comments: pOBD2 + NcoI-PAB1(369-612 bp)-SalI. Expresses Pab1 RRM2 fragment with E181R mutation. Expresses Pab1 fragment fused to GAL4BD.
Proper citation: RRID:Addgene_196835 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: eIF4G1
Vector Backbone Description: Backbone Size:8429; Vector Backbone:pOAD; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25671604
Comments: pOAD + EcoRI-eIF4G1(1-1023)-SalI. (W303 strain as a genomic template). Expresses eIF4G1 N' terminus fused to GAL4AD
Proper citation: RRID:Addgene_196833 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: MCP-2xGFP
Vector Backbone Description: Vector Backbone:pRS305; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36357695
Proper citation: RRID:Addgene_197047 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Superfolder GFP
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:4573; Vector Backbone:HO-poly-KanMX4-HO (ATCC 87804); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36849832
Comments: Supplementary Table 2 of the paper provides details on the cloning of the plasmid.
Proper citation: RRID:Addgene_196616 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: PAH1
Vector Backbone Description: Vector Backbone:UG75; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32859196
Proper citation: RRID:Addgene_198597 Copy
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