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Species: Synthetic
Genetic Insert: RVD sequence: NN NG NG HD
Vector Backbone Description: Vector Backbone:pFUS_B4; Vector Types:Mammalian Expression, TALEN; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:23734242
Comments: Plasmid was created using Golden Gate cloning.
Proper citation: RRID:Addgene_48557 Copy
Species: Synthetic
Genetic Insert: RVD sequence: NN NG NG NI
Vector Backbone Description: Vector Backbone:pFUS_B4; Vector Types:Mammalian Expression, TALEN; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:23734242
Comments: Plasmid was created using Golden Gate cloning.
Proper citation: RRID:Addgene_48556 Copy
Species: Synthetic
Genetic Insert: RVD sequence: NN NG NN NG
Vector Backbone Description: Vector Backbone:pFUS_B4; Vector Types:Mammalian Expression, TALEN; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:23734242
Comments: Plasmid was created using Golden Gate cloning.
Proper citation: RRID:Addgene_48555 Copy
Species: Synthetic
Genetic Insert: RVD sequence: NN NG NN NI
Vector Backbone Description: Vector Backbone:pFUS_B4; Vector Types:Mammalian Expression, TALEN; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:23734242
Comments: Plasmid was created using Golden Gate cloning.
Proper citation: RRID:Addgene_48552 Copy
Species: Synthetic
Genetic Insert: Bacterial TD crRNA to prototspacer B
Vector Backbone Description: Vector Backbone:p15A-cat; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24076762
Proper citation: RRID:Addgene_48656 Copy
Species: Synthetic
Genetic Insert: Bacterial TD crRNA to prototspacer A
Vector Backbone Description: Vector Backbone:p15A-cat; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24076762
Proper citation: RRID:Addgene_48655 Copy
Species: Synthetic
Genetic Insert: Bacterial ST1 crRNA to prototspacer B
Vector Backbone Description: Vector Backbone:p15A-cat; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24076762
Proper citation: RRID:Addgene_48654 Copy
Species: Synthetic
Genetic Insert: Bacterial SP crRNA to prototspacer A
Vector Backbone Description: Vector Backbone:p15A-cat; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24076762
Proper citation: RRID:Addgene_48649 Copy
Species: Synthetic
Genetic Insert: EGFP
Vector Backbone Description: Backbone Size:6600; Vector Backbone:Bluescript; Vector Types:Mammalian Expression, pPy; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23582324
Proper citation: RRID:Addgene_48759 Copy
Species: Synthetic
Genetic Insert: AmCyan
Vector Backbone Description: Backbone Marker:clontech; Backbone Size:4000; Vector Backbone:pmR-mCherry; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: the nAmCyan and the mCherry genes are separated by a P2A sequence that results in 2 separate proteins. It also includes BsmBI restriction sites flanking the nAmCyan gene which result in BsiWI and BssHII overhangs for cloning in-frame with P2A-mCherry.
Proper citation: RRID:Addgene_48686 Copy
Species: Synthetic
Genetic Insert: AcrIIA2
Vector Backbone Description: Vector Backbone:pGEX-6P-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28448066
Proper citation: RRID:Addgene_101042 Copy
Species: Synthetic
Genetic Insert: SpyCas9
Vector Backbone Description: Vector Backbone:pGEX-6P-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28448066
Proper citation: RRID:Addgene_101044 Copy
Species: Synthetic
Genetic Insert: chimeric fungal ITS
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2374; Vector Backbone:pMA-T; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_101174 Copy
Species: Synthetic
Genetic Insert: hSpCas9-eSpCas9(1.1)(K848A/K1003A/R1060A)
Vector Backbone Description: Backbone Marker:Joung lab (Addgene Plasmid #43861); Vector Backbone:JDS246; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28931002
Proper citation: RRID:Addgene_101176 Copy
Species: Synthetic
Genetic Insert: V4 16S rRNA chimeric spike
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2374; Vector Backbone:pMA-T; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_101172 Copy
Species: Synthetic
Genetic Insert: chimeric 18S rRNA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2380; Vector Backbone:pMA-T; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_101173 Copy
Species: Synthetic
Genetic Insert: hSpCas9-Cluster5(K918A/V922A/R925A)
Vector Backbone Description: Backbone Marker:Joung lab (Addgene Plasmid #43861); Vector Backbone:JDS246; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28931002
Proper citation: RRID:Addgene_101191 Copy
Species: Synthetic
Genetic Insert: hSpCas9-Cluster3(T657A/G658A/W659A/R661A)
Vector Backbone Description: Backbone Marker:Joung lab (Addgene Plasmid #43861); Vector Backbone:JDS246; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28931002
Proper citation: RRID:Addgene_101187 Copy
Species: Synthetic
Genetic Insert: hSpCas9-Cluster3+Q926A(T657A/G658A/W659A/R661A/Q926A)
Vector Backbone Description: Backbone Marker:Joung lab (Addgene Plasmid #43861); Vector Backbone:JDS246; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28931002
Proper citation: RRID:Addgene_101188 Copy
Species: Synthetic
Genetic Insert: hSpCas9-Cluster1Q695+Q926A(N692A/M694A/H698A/Q926A)
Vector Backbone Description: Backbone Marker:Joung lab (Addgene Plasmid #43861); Vector Backbone:JDS246; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28931002
Proper citation: RRID:Addgene_101181 Copy
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