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Species: E. coli
Genetic Insert: TadA-RA4.1-SpCas9 D10A
Vector Backbone Description: Backbone Size:3402; Vector Backbone:pCMV with a BR322 origin; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38168987
Proper citation: RRID:Addgene_208306 Copy
Species: E. coli
Genetic Insert: TadA-RA2.1-SpCas9 D10A
Vector Backbone Description: Backbone Size:3402; Vector Backbone:pCMV with a BR322 origin; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38168987
Proper citation: RRID:Addgene_208300 Copy
Species: E. coli
Genetic Insert: TadA-RA3.0-SpCas9 D10A
Vector Backbone Description: Backbone Size:3402; Vector Backbone:pCMV with a BR322 origin; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38168987
Proper citation: RRID:Addgene_208301 Copy
Species: E. coli
Genetic Insert: TadA-RA3.1-SpCas9 D10A
Vector Backbone Description: Backbone Size:3402; Vector Backbone:pCMV with a BR322 origin; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38168987
Proper citation: RRID:Addgene_208302 Copy
Species: E. coli
Genetic Insert: TadA8r
Vector Backbone Description: Backbone Size:5201; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:38168987
Proper citation: RRID:Addgene_208318 Copy
Species: E. coli
Genetic Insert: TadA8e
Vector Backbone Description: Backbone Size:5201; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:38168987
Proper citation: RRID:Addgene_208319 Copy
Species: E. coli
Genetic Insert: TadA-RA5.4-SpCas9 D10A
Vector Backbone Description: Backbone Size:3402; Vector Backbone:pCMV with a BR322 origin; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38168987
Proper citation: RRID:Addgene_208315 Copy
Species: E. coli
Genetic Insert: TadA-RA5.0-SpCas9 D10A
Vector Backbone Description: Backbone Size:3402; Vector Backbone:pCMV with a BR322 origin; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38168987
Proper citation: RRID:Addgene_208312 Copy
Species: E. coli
Genetic Insert: TadA-RA5.1-SpCas9 D10A
Vector Backbone Description: Backbone Size:3402; Vector Backbone:pCMV with a BR322 origin; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38168987
Proper citation: RRID:Addgene_208313 Copy
Species: E. coli
Genetic Insert: TadA8r-R153del-SpCas9 D10A
Vector Backbone Description: Backbone Size:3402; Vector Backbone:pCMV with a BR322 origin; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38168987
Proper citation: RRID:Addgene_208296 Copy
Species: E. coli
Genetic Insert: TadA-RA2.0-SpCas9 D10A
Vector Backbone Description: Backbone Size:3402; Vector Backbone:pCMV with a BR322 origin; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38168987
Proper citation: RRID:Addgene_208299 Copy
Species: E. coli
Genetic Insert: spike-A192
Vector Backbone Description: Backbone Size:5476; Vector Backbone:pET-25b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37001147
Proper citation: RRID:Addgene_200887 Copy
Species: E. coli
Genetic Insert: Cas7, Cas5, Cas8, Cas11, Cas6, and Cas3
Vector Backbone Description: Backbone Size:8900; Vector Backbone:pPV; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31811138
Proper citation: RRID:Addgene_204619 Copy
Species: E. coli
Genetic Insert: E. Coli Mechanosensitive Channel of Large Conductance
Vector Backbone Description: Backbone Size:5600; Vector Backbone:pET19b; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30760590
Proper citation: RRID:Addgene_165097 Copy
Species: E. Coli
Genetic Insert: bsr
Vector Backbone Description: Backbone Size:3057; Vector Backbone:pUPD; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34525356
Comments: CDS
Proper citation: RRID:Addgene_165838 Copy
Species: E. Coli
Genetic Insert: spy
Vector Backbone Description: Backbone Size:2656; Vector Backbone:pUPD4; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34525356
Comments: Bacterial Terminator
Proper citation: RRID:Addgene_165806 Copy
Species: E. coli
Genetic Insert: grpE
Vector Backbone Description: Backbone Size:4493; Vector Backbone:N/A; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:17565681
Comments: Protocol for preparing proteins with improved solubility by co-expressing with molecular chaperones in Escherichia coli.
de Marco A.
Nat Protoc. 2007;2(10):2632-9.
Proper citation: RRID:Addgene_27391 Copy
Species: E. coli
Genetic Insert: dnaK, dnaJ, groESL
Vector Backbone Description: Backbone Size:9177; Vector Backbone:N/A; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:17565681
Comments: Between depositor sequence and Addgene sequencing results, there is a mismatch at position 7284 and an insertion at position 7360. These are in vector backbone and do not alter expression of the inserts.
Protocol for preparing proteins with improved solubility by co-expressing with molecular chaperones in Escherichia coli.
de Marco A.
Nat Protoc. 2007;2(10):2632-9.
Proper citation: RRID:Addgene_27395 Copy
Species: E. coli
Genetic Insert: dnaK, dnaJ, GroESL
Vector Backbone Description: Backbone Size:9203; Vector Backbone:N/A; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:17565681
Comments: Protocol for preparing proteins with improved solubility by co-expressing with molecular chaperones in Escherichia coli.
de Marco A.
Nat Protoc. 2007;2(10):2632-9.
single nt insertion at position 1726.
Proper citation: RRID:Addgene_27396 Copy
Species: E. coli
Genetic Insert: grpE, clpB
Vector Backbone Description: Backbone Size:7194; Vector Backbone:N/A; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:17565681
Comments: Protocol for preparing proteins with improved solubility by co-expressing with molecular chaperones in Escherichia coli.
de Marco A.
Nat Protoc. 2007;2(10):2632-9.
Proper citation: RRID:Addgene_27393 Copy
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