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Species: artifical
Genetic Insert: Gateway-SnAvi
Vector Backbone Description: Backbone Marker:pTriEx5 (Novagen); Backbone Size:6027; Vector Backbone:pBY2887; Vector Types:Mammalian Expression, Bacterial Expression, Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20047968
Proper citation: RRID:Addgene_23222 Copy
Genetic Insert: ccdB
Vector Backbone Description: Vector Backbone:pJFRC7; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: Please see http://www.everyvector.com/sequences/show_public/13276 for additional plasmid map.
Proper citation: RRID:Addgene_41439 Copy
Vector Backbone Description: Backbone Size:9260; Vector Backbone:pLKO; Vector Types:Mammalian Expression, Lentiviral, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: Plasmid for constitutive lentiviral expression.
Alternate plasmid names:
pLX305,
SV40-puro; PGK-gateway-no tag
Proper citation: RRID:Addgene_41390 Copy
Vector Backbone Description: Backbone Size:9354; Vector Backbone:pCW57.1; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination vector, Doxycycline inducible; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: The plasmid expresses rtTA-VP16-2A-puro from the PGK promoter, so it can be used as an 'all-in-one' dox on system.
Alternate plasid names:
pLIX_401
TRE-gateway; PGK-rtTA-2A-puro
Proper citation: RRID:Addgene_41393 Copy
Vector Backbone Description: Backbone Size:7556; Vector Backbone:MSCV; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:18988847
Proper citation: RRID:Addgene_41941 Copy
Genetic Insert: YFP
Vector Backbone Description: Backbone Marker:Eipcentre; Backbone Size:8353; Vector Backbone:pCC1Fos; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:23281894
Comments: Single-copy vector under non-induced condition. Copy-number-inducible with arabinose enables transient induction of copy-number for DNA isolation, etc.
Proper citation: RRID:Addgene_42153 Copy
Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:Eipcentre; Backbone Size:8353; Vector Backbone:pCC1Fos; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:23281894
Comments: Single-copy vector under non-induced condition. Copy-number-inducible with arabinose enables transient induction of copy-number for DNA isolation, etc.
Proper citation: RRID:Addgene_42154 Copy
Genetic Insert: mTFP1
Vector Backbone Description: Backbone Marker:Eipcentre; Backbone Size:8353; Vector Backbone:pCC1Fos; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:23281894
Comments: Single-copy vector under non-induced condition. Copy-number-inducible with arabinose enables transient induction of copy-number for DNA isolation, etc.
Proper citation: RRID:Addgene_42155 Copy
Genetic Insert: CFP
Vector Backbone Description: Backbone Marker:Eipcentre; Backbone Size:8353; Vector Backbone:pCC1Fos; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:23281894
Comments: Single-copy vector under non-induced condition. Copy-number-inducible with arabinose enables transient induction of copy-number for DNA isolation, etc.
Proper citation: RRID:Addgene_42151 Copy
Species: Caenorhabditis elegans
Genetic Insert: mTFP1::[(G4S)3]::C-TAP Tag
Vector Backbone Description: Backbone Marker:Eipcentre; Backbone Size:8353; Vector Backbone:pCC1Fos; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:23281894
Comments: Single-copy vector under non-induced condition. Copy-number-inducible with arabinose enables transient induction of copy-number for DNA isolation, etc.
Proper citation: RRID:Addgene_42166 Copy
Genetic Insert: TALE-TF(VP64)-BB
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV, Adeno-associated Virus (); Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:22828628
Comments: This is a new collection of constructs described in Cong et al. Nature Communications and also an updated TALE Tool Box containing Adeno-associated Virus (AAV) TALE Backbone constructs that allow packaging of TAL transcription factors (TALE-TF) and TALE nucleases (TALEN) into AAV. All constructs are from Feng Zhang lab at Broad Institute/MIT.
IMPORTANT: please note when using the new AAV TALE Backbones, the last 0.5 (half) repeat is not encoded in the backbone vector but instead need to be supplemented as a separate piece.
Proper citation: RRID:Addgene_42581 Copy
Genetic Insert: TALEN-FokI(WT)-BB
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV, Adeno-associated Virus (); Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:22828628
Comments: This is a new collection of constructs described in Cong et al. Nature Communications and also an updated TALE Tool Box containing Adeno-associated Virus (AAV) TALE Backbone constructs that allow packaging of TAL transcription factors (TALE-TF) and TALE nucleases (TALEN) into AAV. All constructs are from Feng Zhang lab at Broad Institute/MIT.
IMPORTANT: please note when using the new AAV TALE Backbones, the last 0.5 (half) repeat is not encoded in the backbone vector but instead need to be supplemented as a separate piece.
Proper citation: RRID:Addgene_42588 Copy
Vector Backbone Description: Backbone Marker:Gateway; Vector Backbone:pPMW; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:29995890
Proper citation: RRID:Addgene_61814 Copy
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Mammalian Expression, Lentiviral, MuLE Gateway Destination Vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25751063
Proper citation: RRID:Addgene_62176 Copy
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Mammalian Expression, Lentiviral, MuLE Gateway Destination Vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25751063
Proper citation: RRID:Addgene_62177 Copy
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Mammalian Expression, Lentiviral, MuLE Gateway Destination Vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25751063
Proper citation: RRID:Addgene_62178 Copy
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Mammalian Expression, Lentiviral, MuLE Gateway Destination Vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25751063
Proper citation: RRID:Addgene_62175 Copy
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4884; Vector Backbone:pBluescript; Vector Types:Unspecified; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25869647
Proper citation: RRID:Addgene_62296 Copy
Vector Backbone Description: Backbone Size:6354; Vector Backbone:pDestTol2pA2 (Tol2Kit#394); Vector Types:CRISPR, Tol2 destination vector for Gateway; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25752963
Comments: Reference for the Tol2Kit:
Kwan, K.M., Fujimoto, E., Grabher, C., Mangum, B.D., Hardy, M.E., Campbell, D.S., Parant, J.M., Yost, H.J., Kanki, J.P., and Chien, C.B. (2007). The Tol2kit: a multisite gateway-based construction kit for Tol2 transposon transgenesis constructs. Dev Dyn 236, 3088-3099.
Proper citation: RRID:Addgene_63157 Copy
Vector Backbone Description: Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Golden Gate Compatible cloning vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24376629
Comments: This plasmid is designed to take up inserts for future Golden Gate cloning using the BsaI enzyme. The 5' BsaI overhang can be exchanged using HindIII and BamHI. The 3' BsaI overhang can be exchanged using KpnI and EcoRI. BamHI and KpnI sites as well as the double XcmI sites can also be used as alternative insert cloning sites, instead of using BsaI.
Plasmid Features (listed as bp in full plasmid sequence):
BsaI site #1 = 2241-2251bp
BsaI site #2 = 3315-3325bp
Proper citation: RRID:Addgene_48863 Copy
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