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Species: Synthetic
Genetic Insert: Reverse rnpB-T1 terminator and forward CP25 promoter for dsRNA expression
Vector Backbone Description: Vector Backbone:colE1; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:32001655
Proper citation: RRID:Addgene_183126 Copy
Species: Bacteriophage MS2
Genetic Insert: MS2 coat protein
Vector Backbone Description: Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:33500316
Proper citation: RRID:Addgene_174786 Copy
Species: Escherichia coli
Genetic Insert: tdk
Vector Backbone Description: Vector Backbone:ColE1; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:32232367
Comments: The tdk-KanR was decribed in Metzgar, David, Jamie M Bacher, Valérie Pezo, John Reader, Volker Döring, Paul Schimmel, Philippe Marlière, and Valérie de Crécy-Lagard. (2004) Acinetobacter Sp. ADP1: An Ideal Model Organism for Genetic Analysis and Genome Engineering. Nucleic Acids Research 32 (19): 5780–5790. https://doi.org/10.1093/nar/gkh881.
Proper citation: RRID:Addgene_180592 Copy
Species: Homo sapiens
Genetic Insert: Lucia Luciferase
Vector Backbone Description: Backbone Marker:Diego Orzaez Lab; Backbone Size:2343; Vector Backbone:pUPD3; Vector Types:Mammalian Expression, Luciferase, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31836712
Comments: Insert can be released with BsaI.
Synthetic luciferase from Invivogen, codon-optimized and synthesized de novo.
Proper citation: RRID:Addgene_135287 Copy
Genetic Insert: Cas12c single guide RNA targeting GFP
Vector Backbone Description: Vector Backbone:p15A ori; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:35659325
Proper citation: RRID:Addgene_183070 Copy
Species: Synthetic
Genetic Insert: Cas12c tracrRNA and pre-crRNA
Vector Backbone Description: Vector Backbone:p15A ori; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:35659325
Comments: A part of the Cas12c2 concatenated noncoding RNA sequence from Addgene plasmid # 120877 (http://n2t.net/addgene:120877 ; RRID:Addgene_120877) was used for tracrRNA expression.
Proper citation: RRID:Addgene_183076 Copy
Species: Synthetic
Genetic Insert: LbCas12a guide RNA containing BbsI sites in spacer sequence
Vector Backbone Description: Vector Backbone:p15A ori; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:35659325
Proper citation: RRID:Addgene_183222 Copy
Species: Synthetic
Genetic Insert: lexA-35S promoter
Vector Backbone Description: Backbone Size:2053; Vector Backbone:mUAV; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_153407 Copy
Vector Backbone Description: Backbone Size:4305; Vector Backbone:pKO403; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:35023783
Comments: Please note that this plasmid runs as a dimer.
Proper citation: RRID:Addgene_174725 Copy
Species: Synthetic
Genetic Insert: MevT-MBIS
Vector Backbone Description: Vector Backbone:p15a; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:33834762
Comments: Please visit https://www.biorxiv.org/content/10.1101/529180v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_139155 Copy
Species: Synthetic
Genetic Insert: sfGFP
Vector Backbone Description: Vector Backbone:SC101; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:33834762
Comments: Please visit https://www.biorxiv.org/content/10.1101/529180v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_139153 Copy
Species: Synthetic
Genetic Insert: Plpp chPyl(IPYE)RS; PproK pylT
Vector Backbone Description: Backbone Marker:Dieter Söll Lab; Backbone Size:1728; Vector Backbone:p15A; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29035361
Proper citation: RRID:Addgene_99222 Copy
Vector Backbone Description: Vector Backbone:n/a; Vector Types:Bacterial Expression, Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34726388
Proper citation: RRID:Addgene_171366 Copy
Species: methanosarcina mazei
Genetic Insert: PylTUUA
Vector Backbone Description: Vector Backbone:pEVOL; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:22628069
Proper citation: RRID:Addgene_182537 Copy
Species: C. difficile / synthetic
Genetic Insert: SmBiT/HupA-LgBiT
Vector Backbone Description: Backbone Marker:R.P. Fagan, University of Sheffield; Vector Backbone:pRPF185; Vector Types:ATc-dependent expression in C. difficile; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30633871
Comments: Please visit https://www.biorxiv.org/content/early/2018/09/27/426809 for bioRxiv preprint.
Proper citation: RRID:Addgene_105497 Copy
Species: C. difficile / synthetic
Genetic Insert: HupA-SmBiT/LgBiT
Vector Backbone Description: Backbone Marker:R.P. Fagan, University of Sheffield; Vector Backbone:pRPF185; Vector Types:ATc-dependent expression in C. difficile; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30633871
Comments: Please visit https://www.biorxiv.org/content/early/2018/09/27/426809 for bioRxiv preprint.
Proper citation: RRID:Addgene_105496 Copy
Species: Synthetic
Genetic Insert: RFP Golden Gate dropout
Vector Backbone Description: Backbone Marker:Deuber Lab; Backbone Size:1661; Vector Backbone:YTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29486239
Comments: Dropout flanked by BsaI sites to allow Deuber Lab MoClo 234 parts to be cloned in using Golden Gate assembly.
Proper citation: RRID:Addgene_105684 Copy
Species: Homo sapiens
Genetic Insert: PRKRA
Vector Backbone Description: Vector Backbone:pLenti_C_Myc_DDK_IRES_Puro; Vector Types:Lentiviral; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29429924
Proper citation: RRID:Addgene_106115 Copy
Species: Homo sapiens
Genetic Insert: PRKRA
Vector Backbone Description: Vector Backbone:pLenti_C_Myc_DDK_IRES_Puro; Vector Types:Lentiviral; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29429924
Proper citation: RRID:Addgene_106114 Copy
Species: Homo sapiens
Genetic Insert: UFD1L
Vector Backbone Description: Vector Backbone:pLenti_C_Myc_DDK_IRES_Puro; Vector Types:Lentiviral; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29429924
Proper citation: RRID:Addgene_106118 Copy
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