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Species: Mus musculus
Genetic Insert: CEBPalpha
Vector Backbone Description: Backbone Marker:Warren Pear lab; Backbone Size:6500; Vector Backbone:NGFR (Addgene plasmid 27489); Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23444353
Proper citation: RRID:Addgene_44627 Copy
Species: Homo sapiens
Genetic Insert: K8
Vector Backbone Description: Vector Backbone:pCIN4-Puro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Please note that Addgene's sequencing results identified two silent mutations in K8 when compared to GenBank accession NM_002273.3. The protein sequence is not affected by these differences.
Proper citation: RRID:Addgene_44626 Copy
Species: Homo sapiens
Genetic Insert: K8
Vector Backbone Description: Vector Backbone:pECE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:2471065
Proper citation: RRID:Addgene_44621 Copy
Species: Homo sapiens
Genetic Insert: K18
Vector Backbone Description: Vector Backbone:pGEM-1; Vector Types:Cloning; Bacterial Resistance:Ampicillin
Defining Citation: PMID:2434380
Proper citation: RRID:Addgene_44623 Copy
Species: Homo sapiens
Genetic Insert: chimeric TRF
Vector Backbone Description: Vector Backbone:pBabe; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23389450
Comments: TRF2 construct in which the DNA-binding domain (aa444-500 when compared to NP_005643.1) was replaced with the TRF1 MYB domain (aa337-439 when compared to NP_059523.2).
According to the depositing laboratory, the deletion of A434 does not affect TRF2 function.
Proper citation: RRID:Addgene_44575 Copy
Species: Homo sapiens
Genetic Insert: chimeric TRF
Vector Backbone Description: Vector Backbone:pBabe; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23389450
Comments: TRF2 construct in which the Hinge domain (aa254-443 when compared to NP_005643.1) was replaced with Hinge domain of TRF1 (aa265-376 when compared to NP_059523.2)
Proper citation: RRID:Addgene_44574 Copy
Species: Homo sapiens
Genetic Insert: chimeric TRF
Vector Backbone Description: Vector Backbone:pBabe; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23389450
Comments: TRF chimera with iDDR region of TRF2 (aa 407-431 compared to GenBank accession number NP_005643.1 - PKGKWNSSNGVEEKETWVEEDELFQ) replacing aa 346-364 of TRF1 (NP_059523.2).
Proper citation: RRID:Addgene_44576 Copy
Species: Homo sapiens
Genetic Insert: KRAS 3'UTR
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4281; Vector Backbone:pGL4.75; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: A substitute for pGL3-KRAS LCS6m in Chin et al., 2008.
Proper citation: RRID:Addgene_44571 Copy
Species: Mus musculus
Genetic Insert: endoB
Vector Backbone Description: Vector Backbone:pUC9; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:2416755
Comments: Original mouse EndoB (k18) cDNA
Proper citation: RRID:Addgene_44602 Copy
Species: Mus musculus
Genetic Insert: mMff isoform 5
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23283981
Proper citation: RRID:Addgene_44601 Copy
Vector Backbone Description: Backbone Size:6433; Vector Backbone:pJDS74; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23478401
Comments: This plasmid is modified pJDS74 vector from "Joung Lab TAL Effector Engineering Reagents" Kit. Modifications are made to the backbone to make it compatible with Voytas Golden Gate TALEN Kit and it can be used in place of pTAL3 or pTAL4. Please see the attached protocol below for modifications.
Proper citation: RRID:Addgene_44715 Copy
Vector Backbone Description: Backbone Size:6433; Vector Backbone:pJDS78; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23478401
Comments: This plasmid is modified pJDS78 vector from "Joung Lab TAL Effector Engineering Reagents" Kit. Modifications are made to the backbone to make it compatible with Voytas Golden Gate TALEN Kit and it can be used in place of pTAL3 or pTAL4. Please see the attached protocol below for modifications.
Proper citation: RRID:Addgene_44716 Copy
Species: Synthetic
Genetic Insert: Cas9-2A-GFP
Vector Backbone Description: Backbone Size:4200; Vector Backbone:pCAG; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23561441
Comments: For more information on Musunuru Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/Musunuru/
Proper citation: RRID:Addgene_44719 Copy
Species: Schmidtea mediterranea
Genetic Insert: EST Contig 5859
Vector Backbone Description: Backbone Size:2961; Vector Backbone:pBluescript SK II (+); Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22074376
Proper citation: RRID:Addgene_44671 Copy
Species: Synthetic
Genetic Insert: FREQ-Seq Barcode: TAGCCA
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23118913
Comments: These plasmids are used to generate bridging primers with Illumina-compatible barcodes for allele frequency sequencing (FREQ-Seq). Similar to RNA-Seq, the FREQ-Seq method can rapidly determine allele frequencies directly from mixed populations with minimal library preparation.
Proper citation: RRID:Addgene_44795 Copy
Species: Schmidtea mediterranea
Genetic Insert: Smed-pp2
Vector Backbone Description: Backbone Size:2961; Vector Backbone:pBluescript SK II (+); Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22074376
Proper citation: RRID:Addgene_44673 Copy
Species: Synthetic
Genetic Insert: FREQ-Seq Barcode: GCTTAG
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23118913
Comments: These plasmids are used to generate bridging primers with Illumina-compatible barcodes for allele frequency sequencing (FREQ-Seq). Similar to RNA-Seq, the FREQ-Seq method can rapidly determine allele frequencies directly from mixed populations with minimal library preparation.
Proper citation: RRID:Addgene_44794 Copy
Species: Schmidtea mediterranea
Genetic Insert: Smed-tplh
Vector Backbone Description: Backbone Size:2961; Vector Backbone:pBluescript SK II (+); Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22074376
Proper citation: RRID:Addgene_44676 Copy
Species: Synthetic
Genetic Insert: FREQ-Seq Barcode: TAACTT
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23118913
Comments: These plasmids are used to generate bridging primers with Illumina-compatible barcodes for allele frequency sequencing (FREQ-Seq). Similar to RNA-Seq, the FREQ-Seq method can rapidly determine allele frequencies directly from mixed populations with minimal library preparation.
Proper citation: RRID:Addgene_44796 Copy
Species: Schmidtea mediterranea
Genetic Insert: EST Contig 6341
Vector Backbone Description: Backbone Size:2961; Vector Backbone:pBluescript SK II (+); Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22074376
Proper citation: RRID:Addgene_44677 Copy
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