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Species: E. coli
Genetic Insert: pLtetO-1:RhyB, pLlacO-1:hfq, pLtetO-1m9:sodB::gfp
Vector Backbone Description: Backbone Marker:Lim Lab (modified from Rolf Lutz and Hermann Bujard, 1997); Backbone Size:2000; Vector Backbone:ColE (from pZ system) + AmpR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21189298
Comments: Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.
Proper citation: RRID:Addgene_30086 Copy
Species: E. coli
Genetic Insert: pLtetO-1:rpoS::mCherry, pLlacO-1:ompC::gfp
Vector Backbone Description: Backbone Marker:Lim Lab (modified from Rolf Lutz and Hermann Bujard, 1997); Backbone Size:2000; Vector Backbone:ColE (from pZ system) + AmpR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21189298
Comments: Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.
Proper citation: RRID:Addgene_30088 Copy
Species: E. coli
Genetic Insert: E. coli elongation factor Tu
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5300; Vector Backbone:pET24c(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:8765000
Proper citation: RRID:Addgene_31172 Copy
Species: E. coli
Genetic Insert: E. coli Initiation Factor 3
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pET21c; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12095986
Proper citation: RRID:Addgene_31340 Copy
Species: E. coli
Genetic Insert: zapB
Vector Backbone Description: Backbone Size:5352; Vector Backbone:pBAD33; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:18394147
Proper citation: RRID:Addgene_31398 Copy
Species: E. coli
Genetic Insert: lacZ ORF with short 5'UTR
Vector Backbone Description: Backbone Size:13150; Vector Backbone:pMS26; Vector Types:Bacterial Expression, bacterial gene addition; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22123741
Comments: Amplification and sequencing primers for verifying insertion into attTn7:
glmS-proximal (Tn7R side)
5’-AATCTGTAACGTTCCGGGTTC-3’ OR
5'-GATGCTGGTGGCGAAGCTGT-3'
pstS-proximal (Tn7L side)
5’-CATTAATAACGAAGAGATGAC-3’ OR
5'-GATGACGGTTTGTCACATGGA-3'
Proper citation: RRID:Addgene_32296 Copy
Species: E. coli
Genetic Insert: BirA
Vector Backbone Description: Backbone Marker:Yves Durocher; Backbone Size:6000; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18296487
Proper citation: RRID:Addgene_32408 Copy
Species: E. coli
Genetic Insert: LacO (256 copies)
Vector Backbone Description: Backbone Marker:Addgene plasmid #33143; Vector Backbone:pVS1; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18614049
Comments: Addgene cannot verify or guarantee the presence of all 256 copies of LacO. This element is unstable.
For use in two-plasmid-based gene-nuclear periphery tethering assay with either plasmids pAK67 (Addgene #33142).
pVS2 contains 256 LacO repeats and a GAL-GFP-GALpA-reporter gene (pVS1 [Addgene #33143] is identical but does not contain the reporter gene). The reporter was previously characterized and contains a GFP open reading frame flanked by the GAL1 promoter and GAL1 3′ UTR ([Abruzzi et al., 2006] and [Dower et al., 2004]).
pKA67 expresses both LacI-GFP and Nup49-GFP to mark the first plasmid as well as the nuclear periphery with GFP.
Proper citation: RRID:Addgene_33144 Copy
Species: E. coli
Genetic Insert: LexA::VP16-SV40 3'UTR
Vector Backbone Description: Vector Backbone:pCasPeR3; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19060186
Comments: A XbaI site was added to the 3' end of LexAVP16-SV40 3'UTR by PCR from pBS-LexAVP16 plasmid (Lai and Lee, 2006).
The PCR fragment was then subcloned into the XbaI site of the pBS vector.
LexAVP16-SV40 was then released by NotI digestion and inserted into the NotI site in pCaspeR3, downstream of Pdf promoter.
Proper citation: RRID:Addgene_33259 Copy
Species: E. coli
Genetic Insert: mEos2-ZapA
Vector Backbone Description: Backbone Marker:NBRP-E.coli at NIG; Backbone Size:4500; Vector Backbone:pCA24N; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25848771
Proper citation: RRID:Addgene_72650 Copy
Species: E. coli
Genetic Insert: ZapB-mEos2
Vector Backbone Description: Backbone Marker:NBRP-E.coli at NIG; Backbone Size:4500; Vector Backbone:pCA24N; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25848771
Proper citation: RRID:Addgene_72651 Copy
Species: E. coli
Genetic Insert: MatP-mEos2
Vector Backbone Description: Backbone Marker:P. de Boer (Hale et al. EMBO J 2001); Backbone Size:7839; Vector Backbone:pDR122; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25848771
Proper citation: RRID:Addgene_72653 Copy
Species: E. coli
Genetic Insert: accA
Vector Backbone Description: Backbone Size:5149; Vector Backbone:pETM6; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23361000
Proper citation: RRID:Addgene_66865 Copy
Species: E. coli
Genetic Insert: OriT
Vector Backbone Description: Backbone Marker:Registry of Standard Parts; Vector Backbone:pSB1A3; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_67964 Copy
Species: E. coli
Genetic Insert: uidA
Vector Backbone Description: Backbone Size:7081; Vector Backbone:pSIM24; Vector Types:Plant Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24897541
Comments: Addgene QC NGS identifies a T120A discrepancy in the GUS insert. It is unknown what, if any, impact this may have on plasmid function.
Proper citation: RRID:Addgene_67882 Copy
Species: E. coli
Genetic Insert: tra Operon (First Quarter)
Vector Backbone Description: Backbone Marker:Registry of Standard Parts; Vector Backbone:pSB1A3; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_67968 Copy
Species: E. coli
Genetic Insert: TraJ
Vector Backbone Description: Backbone Marker:Registry of Standard Parts; Vector Backbone:pSB3C5; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_67965 Copy
Species: E. coli
Genetic Insert: aac3(IV)
Vector Backbone Description: Vector Backbone:pSET152; Vector Types:Streptomyces integrating vector; Bacterial Resistance:Apramycin
Defining Citation: PMID:12923110
Comments: For research using this plasmid, please acknowledge the depositing laboratory and associated citation.
Proper citation: RRID:Addgene_68112 Copy
Species: E. coli
Genetic Insert: folA dihydrofolate reductase
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5708; Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_73188 Copy
Species: E. coli
Genetic Insert: CRP/CAP
Vector Backbone Description: Backbone Marker:NEB; Vector Backbone:pTXB1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_51563 Copy
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