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Species: Other
Genetic Insert: tdh2
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:2319944
Comments: A 2.7 kb HindIII fragment carrying the 2nd tdh gene copy was cloned from the chromosome of Vibrio parahemolyticus strain WP1 into the HindIII site of pBR322
Proper citation: RRID:Addgene_66159 Copy
Species: Other
Genetic Insert: LEE probe A rorf1, espG
Vector Backbone Description: Backbone Size:4109; Vector Backbone:pSPORT1; Vector Types:cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7878036
Comments: E. coli E2348, 3.1 Kb Bg1II-MluI fragment from the left end of the LEE region cloned into the BamHI-MluI site of vector pSPORT I. Contains rorf1 and espG. parent strain: E. coli E2348/69
Proper citation: RRID:Addgene_66154 Copy
Species: Other
Genetic Insert: espA
Vector Backbone Description: Backbone Marker:Qiagen; Backbone Size:3461; Vector Backbone:pQE30; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11956862
Comments: 6XHis N-terminal tagged espA pQE30 (Qiagen). Must be grown in strain that carries the pREP4 repressor plasmid, or harbors the lacIq gene on the F-factor episome (eg XL1-Blue).
Proper citation: RRID:Addgene_66155 Copy
Species: Other
Genetic Insert: ctxB
Vector Backbone Description: Backbone Marker:Kaper lab, Addgene plasmid 63737; Vector Backbone:pCVD315; Vector Types:cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8406837
Comments: ctxB with KpnI linkers cloned into pCVD315 KpnI site. 1.4 kb ctxB probe.plasmid GENOTYPE :pCVD315::ctxB
Proper citation: RRID:Addgene_66162 Copy
Species: Other
Genetic Insert: GFP
Vector Backbone Description: Vector Backbone:pcDNA; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26018130
Proper citation: RRID:Addgene_66589 Copy
Species: Other
Genetic Insert: blasticidin
Vector Backbone Description: Backbone Size:2900; Vector Backbone:pBS SK; Vector Types:Mammalian Expression, Mouse Targeting, CRISPR, TALEN; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28421278
Proper citation: RRID:Addgene_66950 Copy
Species: Other
Genetic Insert: sgRNA and dCas9 from pX330
Vector Backbone Description: Backbone Marker:RIKEN; Backbone Size:8009; Vector Backbone:CSII; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33603226
Proper citation: RRID:Addgene_83306 Copy
Species: Other
Genetic Insert: Strep-FLAG
Vector Backbone Description: Vector Backbone:pKS; Vector Types:Toxoplasma gondii; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19218426
Proper citation: RRID:Addgene_83367 Copy
Species: Other
Genetic Insert: CFP
Vector Backbone Description: Vector Backbone:pKS; Vector Types:Toxoplasma gondii; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19218426
Proper citation: RRID:Addgene_83366 Copy
Species: Other
Genetic Insert: tdTomato
Vector Backbone Description: Vector Backbone:pKS; Vector Types:Toxoplasma gondii; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19218426
Proper citation: RRID:Addgene_83365 Copy
Species: Other
Genetic Insert: Venus
Vector Backbone Description: Backbone Marker:Muller Lab, Addgene plasmid #83798 ; Vector Backbone:pDH22; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12073355
Comments: For more information, see http://yeastrc.org/yeastrc/pages/plasmids_protocols.html
Venus mutations are described in "A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications" by Nagai, Ibata, Park, Kubota, Mikoshiba and Miyawaki in Nat Biotechnol 2002;20:87-90. In addition we have included Q69M, a mutation briefly mentioned in the Nagai et al. paper as potentially beneficial, although not necessary for the enhanced maturation.
Proper citation: RRID:Addgene_83800 Copy
Species: Other
Genetic Insert: CFP
Vector Backbone Description: Backbone Marker:Wach, et al. 1997, PMID 9290211; Backbone Size:4882; Vector Backbone:pFA6a-GFP(S65T)-HIS; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12073355
Comments: For more information, see http://yeastrc.org/yeastrc/pages/plasmids_protocols.html
Proper citation: RRID:Addgene_83789 Copy
Species: Other
Genetic Insert: Cerulean
Vector Backbone Description: Backbone Size:4875; Vector Backbone:pBS4 (Addgene plasmid 83793); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12073355
Comments: For more information, see http://yeastrc.org/yeastrc/pages/plasmids_protocols.html
Proper citation: RRID:Addgene_83795 Copy
Species: Other
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:pBS4 (Addgene plasmid 83793); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12073355
Comments: For more information, see http://yeastrc.org/yeastrc/pages/plasmids_protocols.html
Proper citation: RRID:Addgene_83797 Copy
Species: Other
Genetic Insert: CFP
Vector Backbone Description: Vector Backbone:pyGFP; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12073355
Comments: For more information, see http://yeastrc.org/yeastrc/pages/plasmids_protocols.html
Proper citation: RRID:Addgene_83799 Copy
Species: Other
Genetic Insert: DsRed
Vector Backbone Description: Vector Backbone:pFA6aGFP-KanMX6; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12073355
Comments: For more information, see http://yeastrc.org/yeastrc/pages/plasmids_protocols.html
Proper citation: RRID:Addgene_83794 Copy
Species: Other
Genetic Insert: EGFP
Vector Backbone Description: Vector Backbone:pcDNA3.1/V5-His TOPO TA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17005694
Proper citation: RRID:Addgene_83937 Copy
Species: Other
Genetic Insert: ERT2-spCas9-ERT2
Vector Backbone Description: Backbone Marker:Sytem Biosciences; Vector Backbone:Piggybac Transposon; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Please note that mutation R1058W in Cas9 was found during Addgene's quality control. The depositor noted that this mutation does NOT affect plasmid function.
The plasmid expresses a ERT2-SpCas9-ERT2 tamoxifen inducible Cas9 driven by the EF-1a promoter. T2A linked puromucin enables stable selection of the piggybac transposon. CRISPR gRNAS can be cloned into the vector at the BbsI cloning sites.
Proper citation: RRID:Addgene_83961 Copy
Species: Other
Genetic Insert: Cre
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Cre/Lox, Zebrafish transgenesis; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21497092
Comments: Designed in a pBluescript series vector containing I-SceI meganuclease sites. The I-SceI meganuclease method is a common method for establishing transgenic zebrafish lines.
Please note that the Addgene verified sequence differs from the depositor's reference sequence (see supplemental materials above). The following differences do not alter plasmid functions described in the publication:
- 1kb deletion at the 5' end of the elastase promoter
- 2 other single nucleotide mismatches in elastase promoter
- L88K amino acid residue substitution in the mCherry fluor
- f1 origin is in the opposite orientation
- one mismatch in the ColE1 origin.
Proper citation: RRID:Addgene_84032 Copy
Species: Other
Genetic Insert: hSpCas9
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pCEP4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27472953
Comments: Please note for gRNA cloning: gRNA sequence should be constructed separately in a different u6 containing vector such as pX330, and subcloned into BamHI/NotI sites of pCRISPR-S12.
Proper citation: RRID:Addgene_84031 Copy
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