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Genetic Insert: gateway cassette
Vector Backbone Description: Backbone Marker:see map of pACT2.2 in "author's map"; Backbone Size:7550; Vector Backbone:pACT2.2; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: Gateway-modified yeast two-hybrid Activation domain vector. Use this vector for Gateway cloning of a cDNA to be used to test a directed yeast two-hybrid interaction. This vector was modified from plasmid pACT2.2 by placing a Gateway acceptor cassette in the SmaI site of pACT2.2. To facilitate subcloning of a given DNA insert into this plasmid, Gateway recombinational attachment sites should be incorporated into primers used for amplificiation, as outlined in the Invitrogen Gateway manual. (Gateway is a registered trademark of Invitrogen Corporation.) Please note that the "author's map" is the map of the original pACT2.2 vector upon which pACT2.2gtwy is derived - it is not a map of pACT2.2gtwy.
This plasmid is intended for use exclusively as a teaching resource as part of the Integrated Genomics Discovery-Based Laboratory Course. Addgene does not make any guarantee that the plasmid is suitable for research purposes.
Proper citation: RRID:Addgene_11346 Copy
Genetic Insert: gateway cassette
Vector Backbone Description: Backbone Marker:see map of pLexA in "author's map"; Backbone Size:5700; Vector Backbone:pLexA; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: Gateway-modified yeast two-hybrid DNA-binding domain vector. Use this vector for Gateway cloning of a cDNA to be used as the bait in a yeast two-hybrid screen. This vector was modified from plasmid pLexA by placing a Gateway acceptor cassette in the SmaI site of pLexA. To facilitate subcloning of a given DNA insert into this plasmid, Gateway recombinational attachment sites should be incorporated into primers used for amplificiation, as outlined in the Invitrogen Gateway manual. Gateway recombinational cassettes should be added to primers as outlined in the Invitrogen Gateway manual. (Gateway is a registered trademark of Invitrogen Corporation). Please note that the "author's map" is the map of the original pLexA vector upon which pLexAgtwy is derived - it is not a map of pLexAgtwy.
This plasmid is intended for use exclusively as a teaching resource as part of the Integrated Genomics Discovery-Based Laboratory Course. Addgene does not make any guarantee that the plasmid is suitable for research purposes.
Proper citation: RRID:Addgene_11345 Copy
Genetic Insert: gateway cassette
Vector Backbone Description: Backbone Marker:L4440 is available at Addgene; Backbone Size:2790; Vector Backbone:L4440; Vector Types:Worm Expression, RNAi; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: Gateway-modified RNAi feeding vector L4440. Use this vector for Gateway cloning of an RNAi target sequence. A Gateway recombinational cassette was placed into the EcoRV site of vector L4440 to generate this modified plasmid. To facilitate subcloning of a given DNA insert into this plasmid, Gateway recombinational attachment sites should be incorporated into primers used for amplificiation, as outlined in the Invitrogen Gateway manual. (Gateway is a registered trademark of Invitrogen Corporation.)
This plasmid is intended for use exclusively as a teaching resource as part of the Integrated Genomics Discovery-Based Laboratory Course. Addgene does not make any guarantee that the plasmid is suitable for research purposes.
Proper citation: RRID:Addgene_11344 Copy
Vector Backbone Description: Backbone Marker:FUGW was a gift from David Baltimore (Addgene plasmid # 14883); Backbone Size:9955; Vector Backbone:FUGW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:29997244
Proper citation: RRID:Addgene_113457 Copy
Genetic Insert: ccdB gene, Chloramphenicol resistance gene
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_114018 Copy
Genetic Insert: ccdB gene, Chloramphenicol resistance gene
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_114017 Copy
Genetic Insert: ccdb
Vector Backbone Description: Vector Backbone:I-SceI; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:32709891
Proper citation: RRID:Addgene_118752 Copy
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:5000; Vector Backbone:pGEX-6-P1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_119752 Copy
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:6300; Vector Backbone:pMSCVpuro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:29959219
Proper citation: RRID:Addgene_119745 Copy
Vector Backbone Description: Backbone Marker:Wensheng Wei Lab; Backbone Size:9882; Vector Backbone:pLenti-sgRNA-lib 2.0 (Addgene plasmid #89638); Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:30395134
Proper citation: RRID:Addgene_119976 Copy
Vector Backbone Description: Vector Backbone:pBluescript; Vector Types:Mammalian Expression, CRISPR, piggyBac transposon; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:30639212
Proper citation: RRID:Addgene_120355 Copy
Vector Backbone Description: Backbone Size:12314; Vector Backbone:pPW; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:27006187
Proper citation: RRID:Addgene_124238 Copy
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:6332; Vector Backbone:T-Vector pMD19 (Simple); Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:31740773
Comments: This plasmid is designed for transient transfection of protoplasts. It has only been validated in Zea mays mesophyll protoplasts. The region containing the ccdB suicide gene is dropped out by a double-digest with SacI and KpnI and the large fragment is recombined with the DNA insert library.
Proper citation: RRID:Addgene_117379 Copy
Species: Fluorescent protein genes from jellyfish and coral
Genetic Insert: Brainbow 1.0 L - ccdB survival cassette
Vector Backbone Description: Vector Backbone:pT2-Ip2GI; Vector Types:zebrafish expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:23757414
Proper citation: RRID:Addgene_118221 Copy
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression, Destination; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:30890647
Comments: Please visit https://www.biorxiv.org/content/10.1101/445973v1 for BioRxiv preprint
Proper citation: RRID:Addgene_118375 Copy
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Vector Backbone:pDEST27; Vector Types:Mammalian Expression, Destination; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:30890647
Comments: Please visit https://www.biorxiv.org/content/10.1101/445973v1 for BioRxiv preprint
Proper citation: RRID:Addgene_118374 Copy
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Vector Backbone:pDEST27; Vector Types:Mammalian Expression, Destination; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:30890647
Comments: Please visit https://www.biorxiv.org/content/10.1101/445973v1 for BioRxiv preprint
Proper citation: RRID:Addgene_118367 Copy
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Vector Backbone:pDEST27; Vector Types:Mammalian Expression, Destination; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:30890647
Comments: Please visit https://www.biorxiv.org/content/10.1101/445973v1 for BioRxiv preprint
Proper citation: RRID:Addgene_118373 Copy
Genetic Insert: ccdb
Vector Backbone Description: Vector Backbone:I-SceI; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:32709891
Proper citation: RRID:Addgene_118629 Copy
Genetic Insert: ccdb
Vector Backbone Description: Vector Backbone:I-SceI; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:32709891
Proper citation: RRID:Addgene_118628 Copy
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