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  • Organism:saccharomyces cerevisiae (facet)

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pRS314_U6_G96C_A97C
 
Resource Report
Resource Website 		RRID:Addgene_81235 U6 RNA with G96C/A97C mutation Saccharomyces cerevisiae Ampicillin PMID:27484481 Backbone Size:4500; Vector Backbone:pRS314; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin U6 telestem mutant (G96C/A97C) 2026-08-15 01:20:45 0
CPH3568 (YCp LEU2 Rpb1 P1455_E1456InsGGGGGLEVLFQGP(H)10,N1501K; msLink1)
 
Resource Report
Resource Website 		RRID:Addgene_91819 RPO21 Saccharomyces cerevisiae Ampicillin PMID:28826505 Backbone Marker:ATCC; Backbone Size:7771; Vector Backbone:pRS315; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 5xGly linker, PreScission Protease site (LEVLFQGP), and 10xHis tag inserted between residues P1455 and E1456; changed asparagine 1501 to lysine 2026-08-15 01:22:24 0
CPH3506 (pET151_Spt6 1247-1451 K1355A,K1435A)
 
Resource Report
Resource Website 		RRID:Addgene_91818 SPT6 Saccharomyces cerevisiae Ampicillin PMID:28826505 Backbone Marker:Thermo Fisher Scientific; Backbone Size:5766; Vector Backbone:pET151/D-TOPO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin changed lysine 1355 and lysine 1435 to alanines; deleted residues 1-1246 2026-08-15 01:22:24 0
CPH3567 (pET151_Spt6 1247-1451 R1282H)
 
Resource Report
Resource Website 		RRID:Addgene_91816 SPT6 Saccharomyces cerevisiae Ampicillin PMID:28826505 Backbone Marker:Thermo Fisher Scientific; Backbone Size:5766; Vector Backbone:pET151/D-TOPO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin changed arginine 1282 to histidine; deleted residues 1-1246 2026-08-15 01:22:24 0
CPH3511 (YCp LEU2 Rpb1 P1472A)
 
Resource Report
Resource Website 		RRID:Addgene_91813 RPO21 Saccharomyces cerevisiae Ampicillin PMID:28826505 Backbone Marker:ATCC; Backbone Size:7771; Vector Backbone:pRS315; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin changed proline 1472 to alanine 2026-08-15 01:22:24 0
CPH3509 (YCp LEU2 Rpb1 T1471A)
 
Resource Report
Resource Website 		RRID:Addgene_91812 RPO21 Saccharomyces cerevisiae Ampicillin PMID:28826505 Backbone Marker:ATCC; Backbone Size:7771; Vector Backbone:pRS315; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin changed threonine 1471 to alanine 2026-08-15 01:22:24 0
CPH3488 (YCp LEU2 Rpb1 P1494A)
 
Resource Report
Resource Website 		RRID:Addgene_91810 RPO21 Saccharomyces cerevisiae Ampicillin PMID:28826505 Backbone Marker:ATCC; Backbone Size:7771; Vector Backbone:pRS315; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin changed proline 1494 to alanine 2026-08-15 01:22:24 0
CPH3487 (YCp LEU2 Rpb1 S1493A)
 
Resource Report
Resource Website 		RRID:Addgene_91809 RPO21 Saccharomyces cerevisiae Ampicillin PMID:28826505 Backbone Marker:ATCC; Backbone Size:7771; Vector Backbone:pRS315; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin changed serine 1493 to alanine 2026-08-15 01:22:24 0
CPH3400 (YCp LEU2 Rpb1 A1529_G1534delInsLEVLFQGP)
 
Resource Report
Resource Website 		RRID:Addgene_91806 RPO21 Saccharomyces cerevisiae Ampicillin PMID:28826505 Backbone Marker:ATCC; Backbone Size:7771; Vector Backbone:pRS315; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin Residues A1529-G1534 replaced with a PreScission Protease site (LEVLFQGP) 2026-08-15 01:22:24 0
CPH3477 (YCp LEU2 Rpb1 P1455_E1456InsLEVLFQGP)
 
Resource Report
Resource Website 		RRID:Addgene_91807 RPO21 Saccharomyces cerevisiae Ampicillin PMID:28826505 Backbone Marker:ATCC; Backbone Size:7771; Vector Backbone:pRS315; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin PreScission Protease site (LEVLFQGP) inserted between residues P1455 and E1456 2026-08-15 01:22:24 0
pET28b-Sad1
 
Resource Report
Resource Website 		RRID:Addgene_90297 SAD1 Saccharomyces cerevisiae Kanamycin PMID:24681967 Full-length Sad1, amino acids 1–448, was cloned from S. cerevisiae S288C into the pET-28b plasmid at the Bam HI and XhoI sites, which fuses a 6-His tag to the N terminus. Backbone Marker:EMD Biosciences; Backbone Size:5368; Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:22:06 0
pET28b-ZnF-UBPdel
 
Resource Report
Resource Website 		RRID:Addgene_90299 SAD1 Saccharomyces cerevisiae Kanamycin PMID:24681967 Full-length Sad1, amino acids 1–448, was cloned from S. cerevisiae S288C into the pET-28b plasmid at the Bam HI and XhoI sites, which fuses a 6-His tag to the N terminus. Backbone Marker:EMD Biosciences; Backbone Size:5368; Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin ZnF-UBP deleted 2026-08-15 01:22:06 0
pSL3 - Brr2 del113 N1104L
 
Resource Report
Resource Website 		RRID:Addgene_90253 BRR2 Saccharomyces cerevisiae Ampicillin PMID:27072132 Ledoux and Guthrie, JBC, 2017 Backbone Size:4957; Vector Backbone:pRS313; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin deleted first 113 amino acids and amino acid N1104L 2026-08-15 01:22:05 0
pRS313-Sad1-Wt
 
Resource Report
Resource Website 		RRID:Addgene_90308 SAD1 Saccharomyces cerevisiae Ampicillin PMID:24681967 SAD1 gene with 800 bp upstream of the start site and 900 bp downstream from the stop codon also included Backbone Size:4967; Vector Backbone:pRS313; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:22:06 0
pET28b-sad1-H79A
 
Resource Report
Resource Website 		RRID:Addgene_90303 SAD1 Saccharomyces cerevisiae Kanamycin PMID:24681967 Full-length Sad1, amino acids 1–448, was cloned from S. cerevisiae S288C into the pET-28b plasmid at the Bam HI and XhoI sites, which fuses a 6-His tag to the N terminus. Backbone Marker:EMD Biosciences; Backbone Size:5368; Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin H79A 2026-08-15 01:22:06 0
pSL12 - Brr2 del422 Q904E
 
Resource Report
Resource Website 		RRID:Addgene_90262 BRR2 Saccharomyces cerevisiae Ampicillin PMID:27072132 Ledoux and Guthrie, JBC, 2017 Backbone Size:4957; Vector Backbone:pRS313; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin deleted first 422 amino acids and amino acid Q904E 2026-08-15 01:22:06 0
Sub2-3 in pRS314
 
Resource Report
Resource Website 		RRID:Addgene_90454 SUB2 Saccharomyces cerevisiae Ampicillin PMID:11156604 Addgene NGS results found F165L and Y390F within the SUB2 translation compared to NP_010199.1 Vector Backbone:pRS314; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin sub2-3 mutation 2026-08-15 01:22:07 0
Sub2-6 in pRS314
 
Resource Report
Resource Website 		RRID:Addgene_90456 SUB2 Saccharomyces cerevisiae Ampicillin PMID:11156604 Vector Backbone:pRS314; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin sub2-6 mutation 2026-08-15 01:22:07 0
pWS171
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_90518 Cas9 Saccharomyces cerevisiae Kanamycin For more information see https://benchling.com/pub/ellis-crispr-tools Backbone Size:7255; Vector Backbone:2 micron; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Kanamycin 2026-08-15 01:22:07 2
pWS158
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_90517 Cas9 Saccharomyces cerevisiae Kanamycin For more information see https://benchling.com/pub/ellis-crispr-tools Backbone Size:3831; Vector Backbone:2 micron; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Kanamycin 2026-08-15 01:22:07 5

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