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Species: Spondweni virus
Genetic Insert: Spondweni virus portion of NS2B protease fused to NS3
Vector Backbone Description: Backbone Marker:Centre for Medicines Discovery; Vector Backbone:pNIC-NHSTIIT-GG; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Fusion of SPOV NS2B and NS3 proteases
Proper citation: RRID:Addgene_228656 Copy
Species: Danio rerio
Genetic Insert: Crestin promoter, c-fos minimal promoter, and b-globin intron
Vector Backbone Description: Vector Backbone:pDONR P4-P1r ; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:41230856
Comments: Please visit https://doi.org/10.1101/2024.07.13.603267 for bioRxiv preprint.
Proper citation: RRID:Addgene_229830 Copy
Species: Synthetic
Genetic Insert: attL1/L2 flanked MCS
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:41230856
Comments: Please visit https://doi.org/10.1101/2024.07.13.603267 for bioRxiv preprint.
Proper citation: RRID:Addgene_229832 Copy
Species: Mus musculus
Genetic Insert: c-fos minimal promoter and b-globin intron
Vector Backbone Description: Vector Backbone:pDONR P4-P1r; Vector Types:Mammalian Expression, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:41230856
Comments: Please visit https://doi.org/10.1101/2024.07.13.603267 for bioRxiv preprint.
Proper citation: RRID:Addgene_229829 Copy
Genetic Insert: rpaI
Vector Backbone Description: Vector Backbone:pCOLA; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_213494 Copy
Species: Polio Virus
Genetic Insert: Polio virus 3C protease
Vector Backbone Description: Backbone Marker:Centre for Medicines Discovery; Vector Backbone:pNIC-NOTAG-GG; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_228647 Copy
Species: SARS-CoV-2
Genetic Insert: SARS-CoV-2 N protein (C terminal domain)
Vector Backbone Description: Backbone Marker:Structural Genomics Consortium; Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_228641 Copy
Species: Enterovirus D68
Genetic Insert: Enterovirus D68 3C protease
Vector Backbone Description: Backbone Marker:Centre for Medicines Discovery; Vector Backbone:pNIC-NOTAG-GG; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_228644 Copy
Species: Homo sapiens
Genetic Insert: HTT partial exon 1 Q74-NLS
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:39150509
Comments: Internal plasmid ID GK064
Proper citation: RRID:Addgene_231892 Copy
Species: Enterovirus A71
Genetic Insert: Enterovirus A71 3C protease isoform A
Vector Backbone Description: Backbone Marker:Centre for Medicines Discovery; Vector Backbone:pNIC-NOTAG-GG; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_228635 Copy
Species: Enterovirus A71
Genetic Insert: Enterovirus A71 3C protease isoform B
Vector Backbone Description: Backbone Marker:Centre for Medicines Discovery; Vector Backbone:pNIC-NOTAG-GG; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_228636 Copy
Species: Coxsackievirus 33 isoform B
Genetic Insert: Coxsackievirus 33 isoform B 3C protease
Vector Backbone Description: Backbone Marker:Centre for Medicines Discovery; Vector Backbone:pNIC-NOTAG-GG; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_228639 Copy
Species: Synthetic
Genetic Insert: n2StayGoldc4
Vector Backbone Description: Vector Backbone:pDONR221 / pME; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:41230856
Comments: Please visit https://doi.org/10.1101/2024.07.13.603267 for bioRxiv preprint.
Proper citation: RRID:Addgene_229844 Copy
Species: Enterovirus A71
Genetic Insert: Enterovirus Coxsackievirus A71 2A protease
Vector Backbone Description: Backbone Marker:Centre for Medicines Discovery; Vector Backbone:pNIC-NOTAG-GG; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Uncleavable C-terminal His-Avi tag for purification and biotinylation.
Proper citation: RRID:Addgene_228634 Copy
Species: Rattus norvegicus
Genetic Insert: syt1 (96-421)
Vector Backbone Description: Vector Backbone:pET SUMO; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:38012142
Proper citation: RRID:Addgene_213619 Copy
Species: Homo sapiens
Genetic Insert: DCAF13:M1-K445
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pBiT3.1-C; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:39495097
Comments: 5' Cloning Site: EcoR1 (not destroyed), 3' Cloning Site: Xho1 (not destroyed).
Proper citation: RRID:Addgene_211273 Copy
Species: Rattus norvegicus
Genetic Insert: FL-syt1 5W
Vector Backbone Description: Vector Backbone:pET SUMO; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:38012142
Proper citation: RRID:Addgene_213629 Copy
Species: Rattus norvegicus
Genetic Insert: syt1 (96-421) I367C
Vector Backbone Description: Vector Backbone:pET SUMO; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:38012142
Proper citation: RRID:Addgene_213620 Copy
Species: Rattus norvegicus
Genetic Insert: syt1 5W
Vector Backbone Description: Vector Backbone:pET SUMO; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:38012142
Proper citation: RRID:Addgene_213623 Copy
Species: Mus musculus
Genetic Insert: syt7 C2A-2A 2B
Vector Backbone Description: Vector Backbone:pET SUMO; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:38012142
Proper citation: RRID:Addgene_213626 Copy
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