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Species: Danio rerio
Genetic Insert: muscle-specific promoter of zebrafish unc-45b
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDONRP4-P1R; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23444339
Proper citation: RRID:Addgene_64020 Copy
Species: Danio rerio
Genetic Insert: GFP
Vector Backbone Description: Backbone Marker:Kwan et al., 2007; Vector Backbone:pDestTol2pA2; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:23444339
Proper citation: RRID:Addgene_64022 Copy
Species: Danio rerio
Genetic Insert: zebrafish lymphocyte-specific protein tyrosine kinase (lck) promoter, 7.4 kb
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4777; Vector Backbone:pDONR P4-P1R; Vector Types:Gateway 5' entry clone; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_58891 Copy
Species: Danio rerio
Genetic Insert: mcherry-2a-Rac2D57N
Vector Backbone Description: Backbone Size:3500; Vector Backbone:Tol2; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22014524
Proper citation: RRID:Addgene_58934 Copy
Species: Danio rerio
Genetic Insert: Kit receptor a mouse monoclonal antibody
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:5925; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20525357
Proper citation: RRID:Addgene_58948 Copy
Species: Danio rerio
Genetic Insert: HSPB7
Vector Backbone Description: Vector Backbone:pCRII; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18161059
Comments: linearize with NotI, transcribe with SP6, probe length is 519.
Note: Addgene's quality control sequencing has found a few mismatches in the plasmid insert, but they should not affect its ability to be used as a probe.
Proper citation: RRID:Addgene_61034 Copy
Species: Danio rerio
Genetic Insert: Slit1b
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pBSII SK(+); Vector Types:cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14579375
Comments: antisense probe: cut with SmaI and use T7.
Proper citation: RRID:Addgene_61280 Copy
Species: Danio rerio
Genetic Insert: eno2 regulatory element
Vector Backbone Description: Vector Backbone:pBS; Vector Types:Zebrafish expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17897967
Proper citation: RRID:Addgene_61394 Copy
Species: Danio rerio
Genetic Insert: Cas9-T2A-GFP
Vector Backbone Description: Backbone Size:2765; Vector Backbone:pME-MCS (Tol2Kit #237); Vector Types:CRISPR, Tol2 Middle Entry vector for Gateway; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25752963
Comments: Reference for zebrafish codon-optimized Cas9:
Jao, L.E., Wente, S.R., and Chen, W. (2013). Efficient multiplex biallelic zebrafish genome editing using a CRISPR nuclease system. Proc Natl Acad Sci U S A 110, 13904-13909.
Proper citation: RRID:Addgene_63155 Copy
Species: Danio rerio
Genetic Insert: Cas9
Vector Backbone Description: Backbone Size:2765; Vector Backbone:pME-MCS (Tol2Kit #237); Vector Types:CRISPR, Tol2 Middle Entry vector for Gateway; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25752963
Comments: Reference for zebrafish codon-optimized Cas9:
Jao, L.E., Wente, S.R., and Chen, W. (2013). Efficient multiplex biallelic zebrafish genome editing using a CRISPR nuclease system. Proc Natl Acad Sci U S A 110, 13904-13909.
Proper citation: RRID:Addgene_63154 Copy
Species: Danio rerio
Genetic Insert: friend leukemia integration 1b
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:2515; Vector Backbone:pdonr221; Vector Types:gateway middle entry; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24036310
Proper citation: RRID:Addgene_49019 Copy
Species: Danio rerio
Genetic Insert: Etv5 S142 D
Vector Backbone Description: Backbone Marker:RZPD; Backbone Size:4095; Vector Backbone:pCs2+; Vector Types:Mammalian Expression, xenopus/avian/zebrafish; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20346941
Proper citation: RRID:Addgene_49096 Copy
Species: Danio rerio
Genetic Insert: Dnase1l1l-mRFP
Vector Backbone Description: Backbone Size:4120; Vector Backbone:pCS2; Vector Types:Unspecified; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33854238
Proper citation: RRID:Addgene_169002 Copy
Species: Danio rerio
Genetic Insert: vox partial
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript SK-; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Zvox24-1 cDNA from lambda ZAP vector. Encodes zebrafish partial vox gene. For in situs, cut with EcoRI, transcribe with T7.
Constructed by: David Kimelman, 7/2/96 XVII p.129.
Proper citation: RRID:Addgene_16901 Copy
Species: Danio rerio
Genetic Insert: Her5
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript SK-; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: For probe, digest with XhoI, transcribe with T3 polymerase.
Proper citation: RRID:Addgene_16908 Copy
Species: Danio rerio
Genetic Insert: BMP2b
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript II SK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Probe: cut with EcoRI, transcribe with T3 RNA polymerase.
Proper citation: RRID:Addgene_16915 Copy
Species: Danio rerio
Genetic Insert: snail
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript SK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Antisense probe: cut with EcoR1 and transcribe with T3.
Sense probe: cut with BamH1 and transcribe with T7.
Plasmid History: Constructed by Greg Kelly, 1/94.
Proper citation: RRID:Addgene_16931 Copy
Species: Danio rerio
Genetic Insert: eng2
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:2900; Vector Backbone:pGEM-4; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Antisense probe: cut with EcoR1 and transcribe with T7.
Sense probe: cut with Xba1 and transcribe with SP6.
Plasmid History:
Constructed by Greg Kelly, 11/93.
Proper citation: RRID:Addgene_16930 Copy
Species: Danio rerio
Genetic Insert: goosecoid
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript SK-; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8045345
Comments: Antisense probe cut with EcoR1 and transcribe with T7.
Sense probe cut with Xho1 and transcribe with T3.
Proper citation: RRID:Addgene_16935 Copy
Species: Danio rerio
Genetic Insert: Beta-actin
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript SK+; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Antisense probe cut with EcoR1 and transcribe with T7.
Sense probe cut with Xho1 and transcribe with T3.
Plasmid History:
Constructed by Greg Kelly 5/91.
Proper citation: RRID:Addgene_16932 Copy
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