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Genetic Insert: None
Vector Backbone Description: Backbone Marker:PEL; Backbone Size:8932; Vector Backbone:pDest-560; Vector Types:Bacterial Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_11520 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HSP104
Vector Backbone Description: Backbone Size:4895; Vector Backbone:pRS316; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14978213
Proper citation: RRID:Addgene_1153 Copy
Species: Synthetic
Genetic Insert: RFP
Vector Backbone Description: Backbone Size:4186; Vector Backbone:pBb; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21933410
Comments: JBEI Part ID: JPUB_000096; Origin: SC101
Proper citation: RRID:Addgene_35330 Copy
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4373; Vector Backbone:pRS40x; Vector Types:Yeast integrating vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22670222
Comments: URA3 has been modified to remove ApaI and PstI sites without altering the amino acid sequence of the Ura3 protein.
Proper citation: RRID:Addgene_35442 Copy
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:5496; Vector Backbone:pRS40x; Vector Types:Yeast integrating vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22670222
Comments: LEU2 has been modified to remove ClaI, EcoRI and KpnI sites without altering the amino acid sequence of the Leu2 protein.
Proper citation: RRID:Addgene_35440 Copy
Species: Homo sapiens
Genetic Insert: 4xStat6 promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4267; Vector Backbone:pGL4; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Comments: This plasmid was generated by replacing the HIndIII(blunt)-AdeI fragment of p2xSTAT6-Luc2P (Addgene plasmid 35486) with the KpnI(blunt)-AdeI fragment of p2xSTAT6-Luc2P. The promoter insert consists of four tandem copies of STAT6/cEBP binding sites, a composite element from the human germ line ε promoter.
Lab ID for this plasmid: pANG193.
Proper citation: RRID:Addgene_35554 Copy
Species: Homo sapiens
Genetic Insert: promoter region of miR200b-200a-429 genomic cluster
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4818; Vector Backbone:pGL3-basic; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18829540
Comments: Please note that Addgene's sequencing results identified a single nucleotide mismatch at bp# 210 when compared to the full plasmid sequence provided by the depositing laboratory. This mismatch is not known to affect the function of the plasmid.
Proper citation: RRID:Addgene_35540 Copy
Species: Synthetic
Genetic Insert: ChR1-VChR1 Chimera
Vector Backbone Description: Backbone Size:5385; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21796121
Comments:
Proper citation: RRID:Addgene_35499 Copy
Species: Homo sapiens
Genetic Insert: 3'UTR of ZEB1
Vector Backbone Description: Backbone Marker:Witold Filipowicz; Backbone Size:6380; Vector Backbone:pCI-neo-RL; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18376396
Comments: Mutations in the miR-200b seed regions of the ZEB1 3'UTR were generated using the QuikChange Multi site-directed mutagenesis kit.
The ZEB1 3'UTR was cloned into the plasmid pCIneo-RL which was obtained from Witold Filiowicz.
Proper citation: RRID:Addgene_35537 Copy
Species: Homo sapiens
Genetic Insert: 3'UTR of ZEB2 gene
Vector Backbone Description: Backbone Marker:Witold Filipowicz; Backbone Size:6380; Vector Backbone:pCI-neo-RL; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18376396
Comments: The ZEB2 3'UTR was cloned into the plasmid pCIneo-RL which was obtained from Witold Filiowicz.
Pillai R, Bhattacharyya S, Artus C, Zoller T, Cougot N, Basyuk E, Bertrand E, Filipowicz W (2005) Inhibition of translational initiation by let-7 microRNA in human cells.
Science 309:1573-1576
Please note that Addgene's sequencing results identified a single nucleotide deletion at bp# 2440 when compared to the full plasmid sequence provided by the depositing laboratory. Addgene's sequencing results are correct as the extra base in the sequence from the depositing laboratory was an error due to a sequencing artifact.
Proper citation: RRID:Addgene_35536 Copy
Species: Homo sapiens
Genetic Insert: miR200c-141 genomic cluster
Vector Backbone Description: Backbone Marker:Scott Hammond; Backbone Size:8749; Vector Backbone:pLenti 4.1Ex; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18376396
Proper citation: RRID:Addgene_35534 Copy
Species: Synthetic
Genetic Insert: 7xTOP-GFP
Vector Backbone Description: Backbone Marker:Mark Mercola; Vector Backbone:PGK-H2BmCherry; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22318865
Comments: Please note that the gaps between the Addgene sequencing results and the depositor's full sequence do not alter the plasmid function.
Proper citation: RRID:Addgene_35491 Copy
Species: Homo sapiens
Genetic Insert: KRAS(G12V)
Vector Backbone Description: Backbone Marker:Malcom Moore; Vector Backbone:pUltra; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22318865
Proper citation: RRID:Addgene_35493 Copy
Species: Homo sapiens
Genetic Insert: promoter region of miR200b-200a-429 genomic cluster
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4818; Vector Backbone:pGL3-basic; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18829540
Comments: Please note that Addgene's sequencing results identified a few nucleotide mismatches at bp# 253, 457, 465, 632, 681 & 1463 when compared to the sequence provided by the depositing laboratory. These differences are not known to affect the function of the plasmid.
Proper citation: RRID:Addgene_35539 Copy
Species: Homo sapiens
Genetic Insert: 3'UTR of ZEB2 gene
Vector Backbone Description: Backbone Marker:Witold Filipowicz; Backbone Size:6380; Vector Backbone:pCI-neo-RL; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18376396
Comments: Mutations in the miR-200b seed regions of the ZEB2 3'UTR were generated using the QuikChange Multi site-directed mutagenesis kit.
The ZEB2 3'UTR was cloned into the plasmid pCIneo-RL which was obtained from Witold Filiowicz.
Pillai R, Bhattacharyya S, Artus C, Zoller T, Cougot N, Basyuk E, Bertrand E, Filipowicz W (2005) Inhibition of translational initiation by let-7 microRNA in human cells.
Science 309:1573-1576
Please note that Addgene's sequencing results identified a single nucleotide deletion at bp# 2440 when compared to the full plasmid sequence provided by the depositing laboratory. Addgene's sequencing results are correct as the extra base in the sequence from the depositing laboratory was an error due to a sequencing artifact.
Proper citation: RRID:Addgene_35538 Copy
Species: Homo sapiens
Genetic Insert: DNMT3L
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15861382
Comments: The multiple cloning region of the pcDNA3 vector has been modified. In the modified vector, referred to as pcDNA3/Myc, the region of the multiple cloning site located between the HindIII and NotI sites was replaced by a DNA segment containing a Kozak consensus sequence, an ATG start site and the Myc epitope sequence followed by EcoRI and BamHI restriction sites (see supplemental document). The construction of pcDNA3/Myc has been described in Chen, Z.X., et al. J Cell Biochem, 95: 902-917.
The full-length cDNA for human DNMT3L was cloned into EcoRI and XhoI sites of pcDNA3/Myc. Constructed by Zhaoxia Chen
Proper citation: RRID:Addgene_35523 Copy
Species: Synthetic
Genetic Insert: Vx3K0
Vector Backbone Description: Backbone Marker:clontech; Backbone Size:6300; Vector Backbone:pQCXIX; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22306808
Comments: Please note that the C89S mutation makes the mammalian version different than the bacterially expressed version, but this does not change plasmid function.
Proper citation: RRID:Addgene_35527 Copy
Species: Chlamydomonas reinhardtii
Genetic Insert: hChR2
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:5605; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22179551
Comments:
Proper citation: RRID:Addgene_35510 Copy
Species: Halorubrum sp. TP009
Genetic Insert: eArchT 3.0-EYFP
Vector Backbone Description: Backbone Size:9245; Vector Backbone:pLenti; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22179551
Comments: The enhanced trafficking signals allow for better membrane targeting in mammalian cells resulting in 3-5 fold increase in currents.
Please note that there may be several sequence discrepancies between depositor's reference sequence and Addgene's quality control sequence. These changes are in vector regions only and should not affect plasmid function.
Proper citation: RRID:Addgene_35513 Copy
Species: H. sodomense
Genetic Insert: Arch3.0-EYFP
Vector Backbone Description: Backbone Size:5379; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22179551
Comments: The enhanced trafficking signals allow for better membrane targeting in mammalian cells resulting in 3-5 fold increase in currents.
Proper citation: RRID:Addgene_35516 Copy
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