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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pEGFP-Map7-1333-end Resource Report Resource Website |
RRID:Addgene_46073 | Map7D1-1333-end | Mus musculus | Kanamycin | PMID:22425998 | Backbone Marker:Clontech; Vector Backbone:pEGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:15:28 | 0 | ||
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pEGFP-Map7-Start-1351 Resource Report Resource Website |
RRID:Addgene_46075 | Map7-Start-1351 | Mus musculus | Kanamycin | PMID:22425998 | Discrepancies between the Addgene QC sequence and full sequence do not affect function. | Backbone Marker:Clontech; Vector Backbone:pEGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:15:29 | 0 | |
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pEGFP-Map7-fulllength Resource Report Resource Website 1+ mentions |
RRID:Addgene_46076 | Map7-fulllength | Mus musculus | Kanamycin | PMID:22425998 | Backbone Marker:Clontech; Vector Backbone:pEGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:15:29 | 2 | ||
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pmCIT-CFPGgVcl 1-419 control 2 FRET Resource Report Resource Website |
RRID:Addgene_46283 | ECFP + Vcl 1-419 | Gallus gallus | Kanamycin | PMID:17074767 | Citrine proved to be a more stable FP in vivo than YFP; CIT was used to make a second generation vinculin FRET probe (first generation control is Addgene plasmid 46277). | Backbone Marker:Craig lab; Vector Backbone:pmCitrine-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | aa 1-419 | 2026-08-15 01:15:30 | 0 |
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pmECFPN3/GgVcl 1-1066 (CFP at C term) Resource Report Resource Website |
RRID:Addgene_46280 | Vcl 1-1066 | Gallus gallus | Kanamycin | PMID:15883197 | A206K was introduced into pECFP-N3/V1-1066 by QC to generate pmECFP/V1066 (vinculin-monomeric ECFP) | Backbone Marker:Clonetech; Backbone Size:4700; Vector Backbone:pECFP-N3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:15:30 | 0 | |
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pmECFPC1/GgVcl 1-1066 (CFP at N term) Resource Report Resource Website |
RRID:Addgene_46281 | Vcl 1-1066 | Gallus gallus | Kanamycin | PMID:15883197 | Backbone Marker:Clonetech; Backbone Size:4700; Vector Backbone:pECFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:15:30 | 0 | ||
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pEYFPC1/GgVcl 1-1066 (YFP at N term) Resource Report Resource Website |
RRID:Addgene_46279 | Vcl 1-1066 | Gallus gallus | Kanamycin | PMID:15883197 | Backbone Marker:Clonetech; Backbone Size:4700; Vector Backbone:pEYFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:15:30 | 0 | ||
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HeLa full-length MLCK-GFP Resource Report Resource Website 1+ mentions |
RRID:Addgene_46316 | HeLa long myosin light chain kinase | Homo sapiens | Kanamycin | PMID:15020676 | Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C2; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:15:30 | 2 | ||
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Hela kinase-dead MLCK-GFP Resource Report Resource Website 1+ mentions |
RRID:Addgene_46317 | HeLa long myosin light chain kinase-dead | Homo sapiens | Kanamycin | PMID:15020676 | Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C2; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Kanamycin | E1626K | 2026-08-15 01:15:30 | 3 | |
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pEGFPC1/GgVcl 1-258 A50I mutation Resource Report Resource Website 1+ mentions |
RRID:Addgene_46271 | Vcl 1-258 (aka VD1) A50I mutation | Gallus gallus | Kanamycin | PMID:16608855 | A50I mutation introduced into EGFP/V1-258 (Addgene plasmid 46270) by QCPCR. | Backbone Marker:Clonetech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 1-258 (VD1 domain) A50I mutation | 2026-08-15 01:15:30 | 1 |
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pEGFPC3/GgVcl 884-1066 (Vt) Resource Report Resource Website |
RRID:Addgene_46272 | Vcl 884-1066 (Vt) | Gallus gallus | Kanamycin | PstI-ApaI fragment of pGEX3TV884-1066 subcloned into Pst-Apa cut pEGFP-C3)EGFP. | Backbone Marker:Clonetech; Backbone Size:4700; Vector Backbone:pEGFP-C3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 884-1066 (Vt) tail domain | 2026-08-15 01:15:30 | 0 | |
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pEGFPC1/GgVcl 1-258 (aka VD1) Resource Report Resource Website 1+ mentions |
RRID:Addgene_46270 | Vcl 1-258 (aka VD1) | Gallus gallus | Kanamycin | PMID:16608855 | Nde-Xho fragment from pET15b/Vh1-258 (Addgene plasmid 46173) was subcloned into EGFP/V1-1066 (Addgene plasmid 46265) vector modified to contain unique Nde, Xho sites. Modifications were made by QuikChange PCR to facilitate cloning of cDNAs previously in the pET15b vector. The NdeI site present in the cytomegalovirus promoter region was removed (mutating adenine 234 to thymine), and a new NdeI site was introduced into the multiple cloning site immediately before the vinculin start codon. Additionally, the 5-XhoI site was removed (mutating cytosine 1345 to adenine), and a 3-XhoI site was introduced 3' to the vinculin cDNA in lieu of a PstI site. | Backbone Marker:Clonetech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 1-258 (VD1 domain) | 2026-08-15 01:15:30 | 3 |
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mcherry(C1)_RepoMan Resource Report Resource Website |
RRID:Addgene_46274 | RepoMan | Homo sapiens | Kanamycin | PMID:16492807 | Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:mcherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:15:30 | 0 | ||
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pEGFPC1/GgVcl 1-851 A50I mutant Resource Report Resource Website 1+ mentions |
RRID:Addgene_46269 | Vcl 1-851 A50I mutation | Gallus gallus | Kanamycin | PMID:16608855 | A50I mutation introduced into EGFP/V1-851 (Addgene plasmid 46268) by QCPCR mutation. | Backbone Marker:Clonetech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 1-851 (Vh) head domain, A50I mutation | 2026-08-15 01:15:30 | 2 |
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pEGFPC1/GgVcl 1-1066 T12 mutant Resource Report Resource Website 1+ mentions |
RRID:Addgene_46266 | Vcl T12 mutant | Gallus gallus | Kanamycin | PMID:15728584 | T12 mutation introduced by QuikChange PCR to Addgene plasmid 46265. Mutant inhibits vinculin autoinhibition by ~ 100 fold, vinculin is partially activated and will bind talin, but not actin (unless both talin and actin are present at same time). | Backbone Marker:Clonetech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | T12 mutant (D974;K975;R976; R978A) autoinhibition decreased by 100x | 2026-08-15 01:15:30 | 2 |
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pEGFPC1/GgVcl 1-1066 A50I K1306A, R1308A mutant Resource Report Resource Website |
RRID:Addgene_46267 | Vcl | Gallus gallus | Kanamycin | Chicken vinculin, full length(EcoRI fragment of p1005 subcloned into EcoRI-cut pEGFP-C1); A50I mutation (alanine 50 to isoleucine) introduced by QCPCR. This mutant binds poorly (undetectably to talin in pull down assays; sensitivity of assay Kd= 10-20 uM). | Backbone Marker:Clonetech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | K1306A, R1308A, A50I mutation; talin binding deficient | 2026-08-15 01:15:30 | 0 | |
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pEGFPC1/GgVcl 1-1066 Resource Report Resource Website 1+ mentions |
RRID:Addgene_46265 | Vcl | Gallus gallus | Kanamycin | PMID:15728584 | full length chicken vinculin (EcoRI fragment of p1005 subcloned into EcoRI-cut pEGFP-C1). ATG at bp 1390-1392. TAA at bp 4588-90. EGFP at N-terminus. | Backbone Marker:Clonetech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:15:30 | 1 | |
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pENTR FL CPAP RNAi resistant Resource Report Resource Website |
RRID:Addgene_46390 | CPAP RNAi resistant mutant | Homo sapiens | Kanamycin | PMID:22100914 | Backbone Marker:Invitrogen; Backbone Size:2717; Vector Backbone:pENTR 1A; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin | silent mutation that renders it resistant to siRNA :nucleotides 2862–2880, new sequence: 5′-AGAGTTAGCTAGGATCGAAGA-3′ | 2026-08-15 01:15:31 | 0 | |
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pSEDuet40 Resource Report Resource Website |
RRID:Addgene_46378 | TvoVMA intein | Thermoplasma volcanium | Kanamycin | PMID:22001202 | Backbone Marker:Novagen; Backbone Size:3559; Vector Backbone:pRSFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:15:31 | 0 | ||
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pJDJRSF05 Resource Report Resource Website |
RRID:Addgene_46379 | NpuDnaE intein inactive | Nostoc punctiforme | Kanamycin | PMID:19636943 | Backbone Marker:Novagen; Backbone Size:3559; Vector Backbone:pRSF-1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | C1A | 2026-08-15 01:15:31 | 0 |
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