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pVC0_8_18_Akan(Vn)_(sfgfp)(Vn)
 
Resource Report
Resource Website 		RRID:Addgene_249985 ABR, lv0, Akan, sfGFP Other Kanamycin PMID:40650977 Backbone Marker:Georg Fritz and Thorsten Mascher; Vector Backbone:Synthetic; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin Addgene 2026-09-26 02:54:47 0
pVC0*_D_02_1C-5C sfGFP
 
Resource Report
Resource Website 		RRID:Addgene_250039 dropout part, lv0*, 1R-5O, sfGFP Other Kanamycin PMID:40650977 Backbone Marker:Georg Fritz and Thorsten Mascher; Vector Backbone:Synthetic; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin Addgene 2026-09-26 02:54:47 0
pVC0*_D_03_1R-5O mScarlet-I
 
Resource Report
Resource Website 		RRID:Addgene_250040 dropout part, lv0*, 1C-5C, mScarlet-I Other Kanamycin PMID:40650977 Backbone Marker:Georg Fritz and Thorsten Mascher; Vector Backbone:Synthetic; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin Addgene 2026-09-26 02:54:47 0
CBS-6266
 
Resource Report
Resource Website 		RRID:Addgene_253636 dBa1Cas12a2 E1032A C-terminal His tag Other Kanamycin PMID:41501459 Backbone Marker:Twist; Vector Backbone:pET-29b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin E1032A Addgene 2026-09-26 02:54:48 0
CBS-7173
 
Resource Report
Resource Website 		RRID:Addgene_253638 ca23Cas12a3 E. coli codon optimized C-terminal His tag Other Kanamycin PMID:41501459 Backbone Marker:Twist; Vector Backbone:pET-29b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin N/A Addgene 2026-09-26 02:54:48 0
CBS-7169
 
Resource Report
Resource Website 		RRID:Addgene_253633 MpCas12a2 E. coli codon optimized C-terminal His tag Other Kanamycin PMID:41501459 Vector Backbone:p15A; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin N/A Addgene 2026-09-26 02:54:48 0
CBS-6178
 
Resource Report
Resource Website 		RRID:Addgene_253628 J23119 CAO1 target + GAPDH T17 5x array non-targeting Other Chloramphenicol PMID:41501459 Vector Backbone:p15A; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol N/A Addgene 2026-09-26 02:54:58 0
CBS-6179
 
Resource Report
Resource Website 		RRID:Addgene_253629 J23119 GAPDH T17 target + GAPDH T17 5x array targeting Other Chloramphenicol PMID:41501459 Vector Backbone:p15A; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol N/A Addgene 2026-09-26 02:54:58 0
CBS-6180
 
Resource Report
Resource Website 		RRID:Addgene_253630 J23119 GAPDH T17 target + CAO1 5x array non-targeting Other Chloramphenicol PMID:41501459 Vector Backbone:p15A; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol N/A Addgene 2026-09-26 02:54:58 0
STK032_pSTK-0A-PxylA
 
Resource Report
Resource Website 		RRID:Addgene_235411 PxylA Other Chloramphenicol Backbone Marker:iGEM; Vector Backbone:pSB1A2; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol No Addgene 2026-09-26 02:55:00 0
STK109_pSTK-EXP-2-sfGFP
 
Resource Report
Resource Website 		RRID:Addgene_235362 sfGFP Dropout Cassette Other Chloramphenicol Vector Backbone: pNW33N; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol No Addgene 2026-09-26 02:55:00 0
STK070_pSTK-0D-B0015
 
Resource Report
Resource Website 		RRID:Addgene_235369 Bba_B0015 Other Chloramphenicol Backbone Marker:iGEM; Vector Backbone:pSB1A2; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol No Addgene 2026-09-26 02:55:00 0
STK185_pSTK-0D-S718
 
Resource Report
Resource Website 		RRID:Addgene_235439 S718 term Other Chloramphenicol Backbone Marker:iGEM; Vector Backbone:pSB1A2; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol No Addgene 2026-09-26 02:55:00 0
STK188_pGeoEXP-sfGFP-1
 
Resource Report
Resource Website 		RRID:Addgene_235443 sfGFP Dropout Cassette Other Ampicillin To enable the conjugal transformation of Geobacillus species. plasmid pS797 was engineered to include an origin of transfer (ORI T) that incorporates the Nic region and traJ gene from the RP4 conjugal plasmid. Additionally, it features two replication origins-ColE for E. coli and BST1 for Geobacillus-to support propagation in both hosts. The plasmid also carries two antibiotic resistance markers, which allow for selection with ampicillin in E. coli and kanamycin in Geobacillus. Vector Backbone:pS797; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin No Addgene 2026-09-26 02:55:00 0
STK186_pGeoEXP-LacZ-1
 
Resource Report
Resource Website 		RRID:Addgene_235445 lacZ Dropout Cassette Other Ampicillin To enable the conjugal transformation of Geobacillus species. plasmid pS797 was engineered to include an origin of transfer (ORI T) that incorporates the Nic region and traJ gene from the RP4 conjugal plasmid. Additionally, it features two replication origins-ColE for E. coli and BST1 for Geobacillus-to support propagation in both hosts. The plasmid also carries two antibiotic resistance markers, which allow for selection with ampicillin in E. coli and kanamycin in Geobacillus. Vector Backbone:pS797; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin No Addgene 2026-09-26 02:55:00 0
LentiCRISPR-mCherry-sgLacZ
 
Resource Report
Resource Website 		RRID:Addgene_259664 sgRNA targeting LacZ Other Ampicillin PMID:41946722 Vector Backbone:LentiCRISPRv2puro; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin Addgene 2026-09-26 02:55:23 0
TfCut2_b.45_h90_t.5_i4400_13_80
 
Resource Report
Resource Website 		RRID:Addgene_257146 TfCut2_b.45_h90_t.5_i4400_13_80 Other Kanamycin Vector Backbone:pCDB179; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin S19R, E27T, N28S, R46T, E47S, N48T, N49G, G62A, E64Q, A65S, E72P, I74L, I87N, E98R, N101L, N105D, H106Y, M107L, I108T, S121A, R138E, L139A, Q142D, D145S, T165R, D174E, L175N, K186E, I196L, S197D, I213S, K216T, I217T, T237D Addgene 2026-09-26 02:55:25 0
TfCut2_b.45_h90_t.5_i4400_11_47
 
Resource Report
Resource Website 		RRID:Addgene_257149 TfCut2_b.45_h90_t.5_i4400_11_47 Other Kanamycin Vector Backbone:pCDB179; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin R18S, S19R, R46T, E47S, N48T, N49G, Y51F, G62A, A65S, E72P, I74L, E98R, N101L, N105D, H106Y, M107L, I108T, R138E, L139A, S141E, Q142D, D145S, N158D, T165R, D174E, L175N, T183S, K186E, I196L, S197D, I213S, K216T, I217T, G219A Addgene 2026-09-26 02:55:26 0
TfCut2_b.45_h90_t.5_i4400_14_88
 
Resource Report
Resource Website 		RRID:Addgene_257147 TfCut2_b.45_h90_t.5_i4400_14_88 Other Kanamycin Vector Backbone:pCDB179; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin R18T, S19R, E27T, N28T, R46T, E47S, N48T, N49G, E64Q, A65S, H77Q, I82F, E98R, N101L, N105D, H106Y, M107L, I108T, N109E, S121A, S136T, R138E, L139A, S141K, Q142D, D145S, L175N, K186E, I196L, S197D, I213S, K216T, I217T, V223I Addgene 2026-09-26 02:55:25 0
TfCut2_b.45_h90_t.5_i4400_10_23
 
Resource Report
Resource Website 		RRID:Addgene_257148 TfCut2_b.45_h90_t.5_i4400_10_23 Other Kanamycin Vector Backbone:pCDB179; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin R18S, S19R, S23A, E27T, N28T, R46T, E47S, N48T, N49G, Y51F, A65S, E72P, I87N, E98T, N101L, N105D, H106Y, M107L, I108T, L139A, D145S, T165R, D174E, L175N, I196L, S197D, I213S, K216T, I217T, G219A, V223I, A224S, T237E, R245S Addgene 2026-09-26 02:55:25 0

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