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Species: Other
Genetic Insert: rpoS, mRFP1 and GFPmut3b
Vector Backbone Description: Vector Backbone:P15A; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_63840 Copy
Species: Other
Genetic Insert: GFP
Vector Backbone Description: Vector Backbone:see publication; Vector Types:Yeast Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26934490
Comments: Please see previous FEMS Yeast Research paper for more details:
http://femsyr.oxfordjournals.org/content/14/2/238
Feature list:
T CYC1 Terminator 6..195
T K.l. URA3 Terminator 303..425
Degradation tag 426..476
K. lactis URA3 CDS without STOP codon 477..1277
K. lactis URA3 promoter 1278..1776
Consensus Ty2 3 1966..2152
pUC ori 2939..3582
Consensus Ty2 5 4987..5215
T ADH1 Terminator 5242..5436
pTEF1 Promoter 5455..5874
GFP CDS 5884..6600
Proper citation: RRID:Addgene_64043 Copy
Species: Other
Genetic Insert: MCP
Vector Backbone Description: Vector Backbone:pHAGE-UbiC; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25792328
Proper citation: RRID:Addgene_64541 Copy
Species: Other
Genetic Insert: MCP
Vector Backbone Description: Vector Backbone:pHAGE-UbiC; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25792328
Proper citation: RRID:Addgene_64540 Copy
Species: Other
Genetic Insert: Map2k7 linker MAPK9
Vector Backbone Description: Backbone Marker:John Doench/Kathleen Ottina; Backbone Size:9240; Vector Backbone:pcw107 (V5); Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25538079
Comments: GGCG (N-term barcode, 17 nt upstream of CDS start). Working name: CTK-F2-STOP
Proper citation: RRID:Addgene_64618 Copy
Species: Other
Genetic Insert: Cre-EBD78
Vector Backbone Description: Vector Backbone:pRS303; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23708297
Proper citation: RRID:Addgene_64769 Copy
Species: Other
Genetic Insert: Cre-EBD78
Vector Backbone Description: Vector Backbone:pRS306; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23708297
Comments: The discrepancies between the depositor's sequence and Addgene’s sequences have no functional consequences.
Proper citation: RRID:Addgene_64770 Copy
Species: Other
Genetic Insert: TRANSPORT INHIBITOR RESPONSE 1
Vector Backbone Description: Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23150568
Comments: Nishimura et al., 2009, Nature Methods
Please note that this version does not contains a nuclear export signal. A related construct with the NES can be found at: http://www.addgene.org/47328/
Proper citation: RRID:Addgene_64945 Copy
Species: Other
Genetic Insert: modified AAV-2 rep78
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5238; Vector Backbone:pFastBac-Dual; Vector Types:Insect Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19532142
Comments: Rep78 ORF utilizes a non-canonical initiation codon (CTG codon) beginning at nt 6822.
VP1 capsid ORF utilizes a non-canonical initiation codon (TTG codon) beginning at nt 6514.
Additional article references: Barbash et al. Gene Therapy 20: 274-282 (2013).
Proper citation: RRID:Addgene_65215 Copy
Species: Other
Genetic Insert: modified AAV-2 rep78
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5238; Vector Backbone:pFastBac-Dual; Vector Types:Insect Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19532142
Comments: Rep78 ORF utilizes a non-canonical initiation codon (CTG codon) beginning at nt 6824.
VP1 capsid ORF utilizes a non-canonical initiation codon (ACG codon) beginning at nt 6511.
Proper citation: RRID:Addgene_65214 Copy
Species: Other
Genetic Insert: Streptavidin fused to a signal sequence at 5' end and to a KDEL motif at 3' end
Vector Backbone Description: Backbone Marker:system biosciences; Vector Backbone:pCDH-CMV-MCS-EF1-puro; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22406856
Comments: luminal tagging
Proper citation: RRID:Addgene_65307 Copy
Species: Other
Genetic Insert: Streptavidin fused to a signal sequence at 5' end and to a KDEL motif at 3' end
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pIRESneo3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22406856
Comments: luminal tagging
Proper citation: RRID:Addgene_65306 Copy
Species: Other
Genetic Insert: VSV-G
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pIRESneo3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22406856
Comments: cytoplasmic tagging
Proper citation: RRID:Addgene_65300 Copy
Species: Other
Genetic Insert: RFP
Vector Backbone Description: Backbone Marker:Agilent Technologies; Vector Backbone:pRS416; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25956650
Comments: the RFP gene was used for screening positive transformants(original plasmids would appear red due to expression of RFP, when RFP was replaced by desired inserts, the colony was white)
Proper citation: RRID:Addgene_65319 Copy
Species: Other
Genetic Insert: RFP
Vector Backbone Description: Backbone Marker:Agilent Technologies; Vector Backbone:pRS416; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25956650
Comments: the RFP gene was used for screening positive transformants(original plasmids would appear red due to expression of RFP, when RFP was replaced by desired inserts, the colony was white)
Proper citation: RRID:Addgene_65318 Copy
Species: Other
Genetic Insert: RFP
Vector Backbone Description: Backbone Marker:Agilent Technologies; Vector Backbone:pRS416; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25956650
Comments: the RFP gene was used for screening positive transformants(original plasmids would appear red due to expression of RFP, when RFP was replaced by desired inserts, the colony was white)
Proper citation: RRID:Addgene_65327 Copy
Species: Other
Genetic Insert: RFP
Vector Backbone Description: Backbone Marker:Agilent Technologies; Vector Backbone:pRS416; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25956650
Comments: the RFP gene was used for screening positive transformants(original plasmids would appear red due to expression of RFP, when RFP was replaced by desired inserts, the colony was white)
Proper citation: RRID:Addgene_65323 Copy
Species: Other
Genetic Insert: RFP
Vector Backbone Description: Backbone Marker:Agilent Technologies; Vector Backbone:pRS416; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25956650
Comments: the RFP gene was used for screening positive transformants(original plasmids would appear red due to expression of RFP, when RFP was replaced by desired inserts, the colony was white)
Proper citation: RRID:Addgene_65324 Copy
Species: Other
Genetic Insert: RFP
Vector Backbone Description: Backbone Marker:Agilent Technologies; Vector Backbone:pRS416; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25956650
Comments: the RFP gene was used for screening positive transformants(original plasmids would appear red due to expression of RFP, when RFP was replaced by desired inserts, the colony was white)
Proper citation: RRID:Addgene_65321 Copy
Species: Other
Genetic Insert: RFP
Vector Backbone Description: Backbone Marker:Agilent Technologies; Vector Backbone:pRS416; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25956650
Comments: the RFP gene was used for screening positive transformants(original plasmids would appear red due to expression of RFP, when RFP was replaced by desired inserts, the colony was white)
Proper citation: RRID:Addgene_65320 Copy
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