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Species: Synthetic
Genetic Insert: 3xFLAG-dCas9
Vector Backbone Description: Backbone Marker:Hodaka Fujii; Backbone Size:5791; Vector Backbone:pMXs-puro; Vector Types:Mammalian Expression, Retroviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25051498
Comments: The coding sequence of 3xFLAG-dCas9 can be cleaved with Pac I and Not I.
Construction strategy of gRNA retroviral vectors
1. Cleave gBlock from a gRNA vector constructed using gRNA cloning vector (Addgene #41824) with appropriate restriction enzymes (eg. [Xho I + Hind III], EcoR I).
2. Insert the cleaved gBlock into pSIR-based self-inactivating retroviral vectors.
Vectors & Sites of insertion
pSIR-neo (Addgene #51128): eg. [Xho I + Hind III]
pSIR-GFP (Addgene #51134): eg. [Xho I + Hind III], EcoR I
pSIR-DsRed-Express2 (Addgene #51135): eg. [Xho I + Hind III], EcoR I
pSIR-hCD2 (Addgene #51143): eg. EcoR I
Proper citation: RRID:Addgene_51240 Copy
Species: Mus musculus
Genetic Insert: Gli3
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pEF5-FRT-DEST; Vector Types:Mammalian Expression, Flpin system; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24373970
Proper citation: RRID:Addgene_51247 Copy
Species: Mus musculus
Genetic Insert: Gli3
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pEF5-FRT-DEST; Vector Types:Mammalian Expression, Flpin system; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24373970
Proper citation: RRID:Addgene_51246 Copy
Species: Mus musculus
Genetic Insert: Gli3
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pEF5-FRT-DEST; Vector Types:Mammalian Expression, Flpin system; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24373970
Proper citation: RRID:Addgene_51248 Copy
Species: Homo sapiens
Genetic Insert: Lin28B
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18951094
Comments: N-terminal deletion (70 amino acids)
Proper citation: RRID:Addgene_51374 Copy
Species: Homo sapiens
Genetic Insert: pri-let-7a-1
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18951094
Proper citation: RRID:Addgene_51377 Copy
Species: Mus musculus
Genetic Insert: dynein intermediate chain IC2C
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4731; Vector Backbone:pEGFP-C2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:14565986
Proper citation: RRID:Addgene_51410 Copy
Species: Homo sapiens
Genetic Insert: pri-miR-29b-1~29a
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19079265
Comments: Point mutations: insertion (1115bp), deletion (A47, T666) (Unnecessary region for miRNA biogenesis)
Proper citation: RRID:Addgene_51376 Copy
Species: Homo sapiens
Genetic Insert: Lin28B
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18951094
Proper citation: RRID:Addgene_51373 Copy
Species: Homo sapiens
Genetic Insert: Lin28A
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18951094
Comments: H147A, H169A
Proper citation: RRID:Addgene_51372 Copy
Species: Gallus gallus
Genetic Insert: DCTN1
Vector Backbone Description: Backbone Size:4800; Vector Backbone:pGW1-CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10525538
Proper citation: RRID:Addgene_51412 Copy
Species: Homo sapiens
Genetic Insert: pri-let-7b
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18951094
Proper citation: RRID:Addgene_51378 Copy
Species: Homo sapiens
Genetic Insert: Kinesin-associated protein 3
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pEGFP-C3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16262723
Proper citation: RRID:Addgene_51413 Copy
Species: Gallus gallus
Genetic Insert: Dynamitin
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:6300; Vector Backbone:pCDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10525538
Proper citation: RRID:Addgene_51419 Copy
Species: Homo sapiens
Genetic Insert: pri-let-7e
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18951094
Proper citation: RRID:Addgene_51380 Copy
Species: Homo sapiens
Genetic Insert: DGCR8
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16963499
Proper citation: RRID:Addgene_51386 Copy
Species: Homo sapiens
Genetic Insert: DGCR8
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16963499
Proper citation: RRID:Addgene_51385 Copy
Species: Homo sapiens
Genetic Insert: Lin28A
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19703396
Proper citation: RRID:Addgene_51387 Copy
Species: Homo sapiens
Genetic Insert: pri-let-7g
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18951094
Proper citation: RRID:Addgene_51381 Copy
Species: Homo sapiens
Genetic Insert: DGCR8
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16963499
Comments: Addgene Quality Control sequencing has found some discrepancies in the full plasmid sequence in the V5 tag and linker region of the plasmid. The V5 tag and DGCR8 insert are not effected.
Proper citation: RRID:Addgene_51383 Copy
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