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Species: Synthetic
Genetic Insert: GFP
Vector Backbone Description: Backbone Size:4698; Vector Backbone:AAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16116447
Comments: Plasmid is completely sequenced by the depositing lab except parts of the origin and both ITRs. Multiple digestions were done to verify the vector structure. The construct and the virus were both tested in vitro.
Proper citation: RRID:Addgene_58867 Copy
Species: Bos taurus
Genetic Insert: RNase A
Vector Backbone Description: Backbone Marker:EMD Millipore; Backbone Size:5493; Vector Backbone:pET22b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7479688
Comments: Please note that the pelB sequence is maintained however there are stop codons incorporated at the end of the gene so the His-Tag is not in frame. The pelB is clipped off and the gene encodes 124 amino acid protein that is expressed/purified from inclusion bodies.
Proper citation: RRID:Addgene_58903 Copy
Species: Homo sapiens
Genetic Insert: KRAS
Vector Backbone Description: Backbone Marker:unknown; Backbone Size:5169; Vector Backbone:pBabe-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_58902 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Backbone Size:4895; Vector Backbone:AAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24500276
Comments: The depositing lab has sequenced the 5' ITR, promoter, and fluorophore. Multiple digestions were done to verify the vector structure. The construct was tested in vitro and the virus was tested both in vitro and in vivo.
Proper citation: RRID:Addgene_58909 Copy
Species: Synthetic
Genetic Insert: MESA protease chain with FKBP rapamycin-binding ectodomain, TEV protease and mCherry fusion
Vector Backbone Description: Backbone Size:5521; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24611683
Proper citation: RRID:Addgene_58877 Copy
Species: Mus musculus
Genetic Insert: ZFYVE1 zinc finger, FYVE domain containing 1
Vector Backbone Description: Backbone Marker:Dr.Shoji Yamaoka of Tokyo Medical and Dental University; Backbone Size:6600; Vector Backbone:pMRXIP SECFP-Ci2; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23884233
Proper citation: RRID:Addgene_58765 Copy
Species: S. pyogenes
Genetic Insert: humanized S. pyogenes Cas9 nuclease
Vector Backbone Description: Vector Backbone:pUC ori vector; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24954249
Proper citation: RRID:Addgene_58767 Copy
Species: measles virus
Genetic Insert: Measles virus antigenome
Vector Backbone Description: Vector Backbone:p(+)MV-NSE-FlagP-M502-3p; Vector Types:; Bacterial Resistance:Ampicillin
Comments: Unique AatII site noted in the Modifications to Backbone field was confirmed by restriction digest. Please see Notes from Addgene link to view results.
Proper citation: RRID:Addgene_58800 Copy
Species: S. pyogenes
Genetic Insert: humanized S. pyogenes Cas9 nuclease
Vector Backbone Description: Vector Backbone:pUC ori vector; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24954249
Proper citation: RRID:Addgene_58768 Copy
Species: Stigeoclonium helveticum
Genetic Insert: Chronos-GFP
Vector Backbone Description: Backbone Size:5087; Vector Backbone:AAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24509633
Comments: Plasmid is sequenced completely by the depositing lab except for parts of both ITRs. Multiple digestions were done to verify the vector structure. The construct and the virus were both tested in vitro. They are 10 traditional basepair differences in the CamkII promoter relative to wild-type, which is not a problem.
Proper citation: RRID:Addgene_58805 Copy
Species: S. pyogenes
Genetic Insert: humanized S. pyogenes Cas9 nuclease
Vector Backbone Description: Vector Backbone:pUC ori vector; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:24954249
Proper citation: RRID:Addgene_58779 Copy
Species: Danio rerio
Genetic Insert: zebrafish lymphocyte-specific protein tyrosine kinase (lck) promoter, 7.4 kb
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4777; Vector Backbone:pDONR P4-P1R; Vector Types:Gateway 5' entry clone; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_58891 Copy
Species: S. pyogenes
Genetic Insert: humanized S. pyogenes Cas9 (D10A) nickase
Vector Backbone Description: Vector Backbone:pUC ori vector; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24954249
Proper citation: RRID:Addgene_58772 Copy
Species: Homo sapiens
Genetic Insert: TEM4
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-C2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22911862
Comments: Please note that the Addgene verified sequence identified a L722F mutation in TEM4. It is unknown whether this mutation affects protein function.
Proper citation: RRID:Addgene_58893 Copy
Species: Homo sapiens
Genetic Insert: hDib1
Vector Backbone Description: Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24503441
Proper citation: RRID:Addgene_58787 Copy
Genetic Insert: hDIb1
Vector Backbone Description: Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24503441
Proper citation: RRID:Addgene_58786 Copy
Species: Homo sapiens
Genetic Insert: hDib1
Vector Backbone Description: Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24503441
Proper citation: RRID:Addgene_58788 Copy
Species: S. pyogenes
Genetic Insert: humanized S. pyogenes Cas9 nuclease
Vector Backbone Description: Vector Backbone:pUC ori vector; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:24954249
Proper citation: RRID:Addgene_58781 Copy
Species: S. pyogenes
Genetic Insert: humanized S. pyogenes Cas9 nuclease
Vector Backbone Description: Vector Backbone:pUC ori vector; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:24954249
Proper citation: RRID:Addgene_58782 Copy
Genetic Insert: Nuclear-localized Enhanced Yellow Fluorescent Protein
Vector Backbone Description: Backbone Marker:OTTC/NIDA; Vector Backbone:pOTTC476; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28380331
Proper citation: RRID:Addgene_59133 Copy
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