Searching the RRID Resource Information Network
Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.
Species: E. coli
Genetic Insert: MutS
Vector Backbone Description: Backbone Size:4361; Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:9308969
Comments: pSL6 contains a ClaI–BamHI fragment of pMS312 carrying E. coli mutS and the KanR-conferring HindIII–BamHI fragment of pKC31
Proper citation: RRID:Addgene_28029 Copy
Species: E. Coli
Genetic Insert: FLIPglu-2µΔ13V
Vector Backbone Description: Backbone Size:6866; Vector Backbone:pDRf1GW-ura3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20854260
Proper citation: RRID:Addgene_28000 Copy
Species: E. coli
Genetic Insert: YFP-DHFR(DD)
Vector Backbone Description: Backbone Marker:Garry Nolan; Backbone Size:6943; Vector Backbone:pBMN; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20851347
Proper citation: RRID:Addgene_29326 Copy
Species: E. coli
Genetic Insert: EcoRI endonuclease & EcoRI methylase
Vector Backbone Description: Backbone Marker:n/a; Backbone Size:3000; Vector Backbone:pSCC3; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:2745418
Comments: pSCC3 plasmid derivation is described in King, K. et al. (1989) J.Biol.Chem. 264, 11807-11815.
Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.
Proper citation: RRID:Addgene_40190 Copy
Species: E. Coli
Genetic Insert: Bistable switch using dynamic degradation
Vector Backbone Description: Backbone Size:3460; Vector Backbone:psc101; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22776405
Proper citation: RRID:Addgene_40108 Copy
Species: E. Coli
Genetic Insert: CI inducible sfGFP
Vector Backbone Description: Backbone Size:2097; Vector Backbone:p15A; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22776405
Proper citation: RRID:Addgene_40127 Copy
Species: E. Coli
Genetic Insert: Lac inducible lambda repressor
Vector Backbone Description: Backbone Size:4147; Vector Backbone:ColE1; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:22776405
Proper citation: RRID:Addgene_40126 Copy
Species: E. coli
Genetic Insert: GFP-LacI**
Vector Backbone Description: Backbone Marker:Andrew W. Murray Lab, UCSF (Straight, Sedat, & Murray, 1998. PMID: 9813090); Backbone Size:5650; Vector Backbone:pAFS135; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21724830
Comments: The plasmid pAFS135 (Straight, Sedat, and Murray, 1998) contains GFP(S65T, V163A)-LacI(P3Y, del C-terminal 11 aa)-SV40 NLS under the control of a HIS3 promoter fragment. To construct pAT123, the HIS3 marker of AFS135 was replaced with a ScaI-DraIII fragment from RS405 containing LEU2 (a kind gift from Karine Dubrana and Susan Gasser).
The plasmid pAT222 was obtained by site-directed mutagenesis of pAT123 to create GFP-LacI**, using the primers (base mutations in lowercase):
5’- GTGGCACAgCAACTGGCGGaCAAAggtggcggaTCGTTGCTGATTGGCGTTGCCtCCT-3’ and
5’- AGGaGGCAACGCCAATCAGCAACGAtccgccaccTTTGtCCGCCAGTTGcTGTGCCAC- 3’.
Alternate plasmid name: pAT123LacI2G(4mutations)
Proper citation: RRID:Addgene_40942 Copy
Species: E. coli
Genetic Insert: lacZ ORF with short 5'UTR
Vector Backbone Description: Backbone Size:13150; Vector Backbone:pMS26; Vector Types:Bacterial Expression, bacterial gene addition; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22123741
Comments: Amplification and sequencing primers for verifying insertion into attTn7:
glmS-proximal (Tn7R side)
5’-AATCTGTAACGTTCCGGGTTC-3’ OR
5'-GATGCTGGTGGCGAAGCTGT-3'
pstS-proximal (Tn7L side)
5’-CATTAATAACGAAGAGATGAC-3’ OR
5'-GATGACGGTTTGTCACATGGA-3'
Proper citation: RRID:Addgene_32296 Copy
Species: E. coli
Genetic Insert: BirA
Vector Backbone Description: Backbone Marker:Yves Durocher; Backbone Size:6000; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18296487
Proper citation: RRID:Addgene_32408 Copy
Species: E. coli
Genetic Insert: LacO (256 copies)
Vector Backbone Description: Backbone Marker:Addgene plasmid #33143; Vector Backbone:pVS1; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18614049
Comments: Addgene cannot verify or guarantee the presence of all 256 copies of LacO. This element is unstable.
For use in two-plasmid-based gene-nuclear periphery tethering assay with either plasmids pAK67 (Addgene #33142).
pVS2 contains 256 LacO repeats and a GAL-GFP-GALpA-reporter gene (pVS1 [Addgene #33143] is identical but does not contain the reporter gene). The reporter was previously characterized and contains a GFP open reading frame flanked by the GAL1 promoter and GAL1 3′ UTR ([Abruzzi et al., 2006] and [Dower et al., 2004]).
pKA67 expresses both LacI-GFP and Nup49-GFP to mark the first plasmid as well as the nuclear periphery with GFP.
Proper citation: RRID:Addgene_33144 Copy
Species: E. coli
Genetic Insert: LexA::VP16-SV40 3'UTR
Vector Backbone Description: Vector Backbone:pCasPeR3; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19060186
Comments: A XbaI site was added to the 3' end of LexAVP16-SV40 3'UTR by PCR from pBS-LexAVP16 plasmid (Lai and Lee, 2006).
The PCR fragment was then subcloned into the XbaI site of the pBS vector.
LexAVP16-SV40 was then released by NotI digestion and inserted into the NotI site in pCaspeR3, downstream of Pdf promoter.
Proper citation: RRID:Addgene_33259 Copy
Species: E. coli
Genetic Insert: LexAp65
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2664; Vector Backbone:pDONR P4r-P3r; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_41438 Copy
Species: E. coli
Genetic Insert: rpoN
Vector Backbone Description: Backbone Marker:novagen; Backbone Size:5708; Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22341441
Proper citation: RRID:Addgene_41748 Copy
Species: E. coli
Genetic Insert: ptet_ompC_gfp
Vector Backbone Description: Backbone Size:2000; Vector Backbone:ColE (from pZ system) + AmpR; Vector Types:Synthetic Biology, E. coli; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22618873
Proper citation: RRID:Addgene_37757 Copy
Species: E. coli
Genetic Insert: pLar_rpoS_gfp
Vector Backbone Description: Backbone Size:2000; Vector Backbone:ColE (from pZ system) + AmpR; Vector Types:Synthetic Biology, E. coli; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22618873
Proper citation: RRID:Addgene_37758 Copy
Species: E. coli
Genetic Insert: ptet_ompC_gfp
Vector Backbone Description: Backbone Size:2000; Vector Backbone:ColE (from pZ system) + AmpR; Vector Types:Synthetic Biology, E. coli; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22618873
Proper citation: RRID:Addgene_37754 Copy
Species: E. coli
Genetic Insert: DsbC
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:p15A; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22197595
Proper citation: RRID:Addgene_38152 Copy
Species: E. coli
Genetic Insert: birA
Vector Backbone Description: Backbone Marker:Matthew Bogyo laboratory; Backbone Size:6126; Vector Backbone:Addgene Plasmid #38251 (pET22b-CPDSalI); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19956581
Comments: Encodes untagged biotin ligase gene (birA) from E. coli fused to Vibrio cholerae MARTX toxin cysteine protease domain (CPD) to be used to purify biotin ligase.
Proper citation: RRID:Addgene_38258 Copy
Species: E. coli
Genetic Insert: BirA::GFP
Vector Backbone Description: Backbone Size:2884; Vector Backbone:pWormgate2; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22219512
Proper citation: RRID:Addgene_45100 Copy
Can't find your Plasmid?
We recommend that you click next to the search bar to check some helpful tips on searches and refine your search firstly. If you want to find a specific plasmid, it's easier to enter an RRID or an Addgene Catalog Number to search. You can refine the search results using Facets on the left side of the search results page. If you are on the table view, you can also search in a specific column by clicking the column title and enter the keywords.
If you still could not find your plasmid in the search results, please help us by registering it into the system — it's easy. Register it with Addgene.
Welcome to the RRID Resources search. From here you can search through a compilation of resources used by RRID and see how data is organized within our community.
You are currently on the Community Resources tab looking through categories and sources that RRID has compiled. You can navigate through those categories from here or change to a different tab to execute your search through. Each tab gives a different perspective on data.
If you have an account on RRID then you can log in from here to get additional features in RRID such as Collections, Saved Searches, and managing Resources.
Here is the search term that is being executed, you can type in anything you want to search for. Some tips to help searching:
You can save any searches you perform for quick access to later from here.
We recognized your search term and included synonyms and inferred terms along side your term to help get the data you are looking for.
If you are logged into RRID you can add data records to your collections to create custom spreadsheets across multiple sources of data.
Here are the sources that were queried against in your search that you can investigate further.
Here are the categories present within RRID that you can filter your data on
Here are the subcategories present within this category that you can filter your data on
If you have any further questions please check out our FAQs Page to ask questions and see our tutorials. Click this button to view this tutorial again.
