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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pSL6 SMR1747 Resource Report Resource Website |
RRID:Addgene_28029 | MutS | E. coli | Kanamycin | PMID:9308969 | pSL6 contains a ClaI–BamHI fragment of pMS312 carrying E. coli mutS and the KanR-conferring HindIII–BamHI fragment of pKC31 | Backbone Size:4361; Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:13:10 | 0 | |
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pDR-GW FLIPglu-2µΔ13V Resource Report Resource Website |
RRID:Addgene_28000 | FLIPglu-2µΔ13V | E. Coli | Ampicillin | PMID:20854260 | Backbone Size:6866; Vector Backbone:pDRf1GW-ura3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | H152A | 2026-08-15 01:13:08 | 0 | |
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pBMN YFP-DHFR(DD) Resource Report Resource Website 1+ mentions |
RRID:Addgene_29326 | YFP-DHFR(DD) | E. coli | Ampicillin | PMID:20851347 | Backbone Marker:Garry Nolan; Backbone Size:6943; Vector Backbone:pBMN; Vector Types:Retroviral; Bacterial Resistance:Ampicillin | Change Arg 12 to His (R12H),Asn 18 Thr (N18T), Val 19 Ala (V19A) and Gly 67 to Ser (G67S)--destabilizing domain | 2026-08-15 01:13:18 | 5 | |
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pEQ111m Resource Report Resource Website 1+ mentions |
RRID:Addgene_40190 | EcoRI endonuclease & EcoRI methylase | E. coli | Ampicillin | PMID:2745418 | pSCC3 plasmid derivation is described in King, K. et al. (1989) J.Biol.Chem. 264, 11807-11815. Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions. | Backbone Marker:n/a; Backbone Size:3000; Vector Backbone:pSCC3; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | changed Glutamic Acid 111 to Glutamine | 2026-08-15 01:14:34 | 2 |
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SW5 Resource Report Resource Website |
RRID:Addgene_40108 | Bistable switch using dynamic degradation | E. Coli | Kanamycin | PMID:22776405 | Backbone Size:3460; Vector Backbone:psc101; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin | 2026-08-15 01:14:33 | 0 | ||
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PRM-GFP Resource Report Resource Website 1+ mentions |
RRID:Addgene_40127 | CI inducible sfGFP | E. Coli | Ampicillin | PMID:22776405 | Backbone Size:2097; Vector Backbone:p15A; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:33 | 2 | ||
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PLlacO-1-CImf Resource Report Resource Website |
RRID:Addgene_40126 | Lac inducible lambda repressor | E. Coli | Chloramphenicol | PMID:22776405 | Backbone Size:4147; Vector Backbone:ColE1; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol | 2026-08-15 01:14:33 | 0 | ||
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pAT222 Resource Report Resource Website |
RRID:Addgene_40942 | GFP-LacI** | E. coli | Ampicillin | PMID:21724830 | The plasmid pAFS135 (Straight, Sedat, and Murray, 1998) contains GFP(S65T, V163A)-LacI(P3Y, del C-terminal 11 aa)-SV40 NLS under the control of a HIS3 promoter fragment. To construct pAT123, the HIS3 marker of AFS135 was replaced with a ScaI-DraIII fragment from RS405 containing LEU2 (a kind gift from Karine Dubrana and Susan Gasser). The plasmid pAT222 was obtained by site-directed mutagenesis of pAT123 to create GFP-LacI**, using the primers (base mutations in lowercase): 5’- GTGGCACAgCAACTGGCGGaCAAAggtggcggaTCGTTGCTGATTGGCGTTGCCtCCT-3’ and 5’- AGGaGGCAACGCCAATCAGCAACGAtccgccaccTTTGtCCGCCAGTTGcTGTGCCAC- 3’. Alternate plasmid name: pAT123LacI2G(4mutations) | Backbone Marker:Andrew W. Murray Lab, UCSF (Straight, Sedat, & Murray, 1998. PMID: 9813090); Backbone Size:5650; Vector Backbone:pAFS135; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | LacI**/LacI2G(4 mutations) (see comment below); lacI-I12 mutation (P3Y); C-terminal 11 amino acids deleted to prevent tetramerization | 2026-08-15 01:14:40 | 0 |
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pMS33 Resource Report Resource Website |
RRID:Addgene_32296 | lacZ ORF with short 5'UTR | E. coli | Ampicillin | PMID:22123741 | Amplification and sequencing primers for verifying insertion into attTn7: glmS-proximal (Tn7R side) 5’-AATCTGTAACGTTCCGGGTTC-3’ OR 5'-GATGCTGGTGGCGAAGCTGT-3' pstS-proximal (Tn7L side) 5’-CATTAATAACGAAGAGATGAC-3’ OR 5'-GATGACGGTTTGTCACATGGA-3' | Backbone Size:13150; Vector Backbone:pMS26; Vector Types:Bacterial Expression, bacterial gene addition; Bacterial Resistance:Ampicillin | 2026-08-15 01:13:40 | 0 | |
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secretedBirA-8his Resource Report Resource Website 1+ mentions |
RRID:Addgene_32408 | BirA | E. coli | Ampicillin | PMID:18296487 | Backbone Marker:Yves Durocher; Backbone Size:6000; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:13:41 | 7 | ||
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pVS2 Resource Report Resource Website |
RRID:Addgene_33144 | LacO (256 copies) | E. coli | Ampicillin | PMID:18614049 | Addgene cannot verify or guarantee the presence of all 256 copies of LacO. This element is unstable. For use in two-plasmid-based gene-nuclear periphery tethering assay with either plasmids pAK67 (Addgene #33142). pVS2 contains 256 LacO repeats and a GAL-GFP-GALpA-reporter gene (pVS1 [Addgene #33143] is identical but does not contain the reporter gene). The reporter was previously characterized and contains a GFP open reading frame flanked by the GAL1 promoter and GAL1 3′ UTR ([Abruzzi et al., 2006] and [Dower et al., 2004]). pKA67 expresses both LacI-GFP and Nup49-GFP to mark the first plasmid as well as the nuclear periphery with GFP. | Backbone Marker:Addgene plasmid #33143; Vector Backbone:pVS1; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:13:47 | 0 | |
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pCasper_Pdf-LexA::VP16 Resource Report Resource Website |
RRID:Addgene_33259 | LexA::VP16-SV40 3'UTR | E. coli | Ampicillin | PMID:19060186 | A XbaI site was added to the 3' end of LexAVP16-SV40 3'UTR by PCR from pBS-LexAVP16 plasmid (Lai and Lee, 2006). The PCR fragment was then subcloned into the XbaI site of the pBS vector. LexAVP16-SV40 was then released by NotI digestion and inserted into the NotI site in pCaspeR3, downstream of Pdf promoter. | Vector Backbone:pCasPeR3; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:13:48 | 0 | |
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R4-LexAp65-R3 Resource Report Resource Website |
RRID:Addgene_41438 | LexAp65 | E. coli | Kanamycin | Backbone Marker:Invitrogen; Backbone Size:2664; Vector Backbone:pDONR P4r-P3r; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:14:45 | 0 | |||
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pSigma54-4 Resource Report Resource Website |
RRID:Addgene_41748 | rpoN | E. coli | Ampicillin | PMID:22341441 | Backbone Marker:novagen; Backbone Size:5708; Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | c198a c307g c346a | 2026-08-15 01:14:48 | 0 | |
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pHL 279 Resource Report Resource Website |
RRID:Addgene_37757 | ptet_ompC_gfp | E. coli | Ampicillin | PMID:22618873 | Backbone Size:2000; Vector Backbone:ColE (from pZ system) + AmpR; Vector Types:Synthetic Biology, E. coli; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:16 | 0 | ||
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pHL 363 Resource Report Resource Website |
RRID:Addgene_37758 | pLar_rpoS_gfp | E. coli | Ampicillin | PMID:22618873 | Backbone Size:2000; Vector Backbone:ColE (from pZ system) + AmpR; Vector Types:Synthetic Biology, E. coli; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:16 | 0 | ||
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pHL 233 Resource Report Resource Website |
RRID:Addgene_37754 | ptet_ompC_gfp | E. coli | Ampicillin | PMID:22618873 | Backbone Size:2000; Vector Backbone:ColE (from pZ system) + AmpR; Vector Types:Synthetic Biology, E. coli; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:16 | 0 | ||
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pBA2219 Resource Report Resource Website |
RRID:Addgene_38152 | DsbC | E. coli | Kanamycin | PMID:22197595 | Backbone Marker:Novagen; Vector Backbone:p15A; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:14:19 | 0 | ||
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pET22b-BirA-CPDSalI Resource Report Resource Website |
RRID:Addgene_38258 | birA | E. coli | Ampicillin | PMID:19956581 | Encodes untagged biotin ligase gene (birA) from E. coli fused to Vibrio cholerae MARTX toxin cysteine protease domain (CPD) to be used to purify biotin ligase. | Backbone Marker:Matthew Bogyo laboratory; Backbone Size:6126; Vector Backbone:Addgene Plasmid #38251 (pET22b-CPDSalI); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | cloned from pGEX4T1-BirA | 2026-08-15 01:14:20 | 0 |
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pSO221 Phis-72 BirA GFP Resource Report Resource Website |
RRID:Addgene_45100 | BirA::GFP | E. coli | Ampicillin | PMID:22219512 | Backbone Size:2884; Vector Backbone:pWormgate2; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:15:21 | 0 |
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