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Species: Synthetic
Genetic Insert: hSpCas9-Cluster2+Q926A(G582A/V583A/E584A/D585A/N588A/Q926A)
Vector Backbone Description: Backbone Marker:Joung lab (Addgene Plasmid #43861); Vector Backbone:JDS246; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28931002
Proper citation: RRID:Addgene_101184 Copy
Species: Synthetic
Genetic Insert: hSpCas9-Cluster4(F491A/M495A/T496A/N497A)
Vector Backbone Description: Backbone Marker:Joung lab (Addgene Plasmid #43861); Vector Backbone:JDS246; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28931002
Proper citation: RRID:Addgene_101189 Copy
Species: Synthetic
Genetic Insert: hSpCas9-Cluster5+Q926A(K918A/V922A/R925A/Q926A)
Vector Backbone Description: Backbone Marker:Joung lab (Addgene Plasmid #43861); Vector Backbone:JDS246; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28931002
Proper citation: RRID:Addgene_101192 Copy
Species: Synthetic
Genetic Insert: LOV2 and STIM1 cytosolic fragment
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pTriEx; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26646180
Proper citation: RRID:Addgene_101245 Copy
Species: Synthetic
Genetic Insert: ASAP2s
Vector Backbone Description: Backbone Size:4562; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28749338
Comments: The next-generation JEDI-2P sensors are available at: https://www.addgene.org/browse/article/28223314/
Proper citation: RRID:Addgene_101276 Copy
Species: Synthetic
Genetic Insert: MRLC-DD and AP4-Mito
Vector Backbone Description: Vector Backbone:pcDNA3.1(-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25065757
Comments: MRLC-DD: Myosin regulatory light-chain transgene and AP4-Mito:a transgene bearing ActA AP4 motifs fused to a mitochondrial targeting sequence (INACTIVE)
Proper citation: RRID:Addgene_101287 Copy
Species: Synthetic
Genetic Insert: LRP6 Signal peptide, MCS linker, Twin-Strep tag
Vector Backbone Description: Backbone Marker:Geneva Biotech; Vector Backbone:pACEBac1; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:29143175
Proper citation: RRID:Addgene_101652 Copy
Species: Synthetic
Genetic Insert: LRP6 Signal peptide, MCS linker, 3xFLAG tag
Vector Backbone Description: Vector Backbone:pACEBac1; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:29143175
Proper citation: RRID:Addgene_101653 Copy
Species: Synthetic
Genetic Insert: LRP6 Signal peptide, KpnI linker, 3xFLAG tag
Vector Backbone Description: Backbone Marker:Geneva Biotech; Vector Backbone:pACEBac1; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:29143175
Proper citation: RRID:Addgene_101654 Copy
Species: Synthetic
Genetic Insert: crRNAs targeting GFP and mCherry
Vector Backbone Description: Backbone Size:2870; Vector Backbone:pCRRNAcos; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29519933
Proper citation: RRID:Addgene_101817 Copy
Species: Synthetic
Genetic Insert: crRNAs targeting GFP and mCherry
Vector Backbone Description: Backbone Size:2870; Vector Backbone:pCRRNAcos; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29519933
Proper citation: RRID:Addgene_101818 Copy
Species: Synthetic
Genetic Insert: crRNAs targeting GFP and mCherry
Vector Backbone Description: Backbone Size:2870; Vector Backbone:pCRRNAcos; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29519933
Proper citation: RRID:Addgene_101815 Copy
Species: Synthetic
Genetic Insert: crRNAs targeting GFP and mCherry
Vector Backbone Description: Backbone Size:2870; Vector Backbone:pCRRNAcos; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29519933
Proper citation: RRID:Addgene_101816 Copy
Species: Synthetic
Genetic Insert: crRNAs targeting GFP and mCherry
Vector Backbone Description: Backbone Size:2870; Vector Backbone:pCRRNAcos; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29519933
Proper citation: RRID:Addgene_101811 Copy
Species: Synthetic
Genetic Insert: crRNAs targeting GFP and mCherry
Vector Backbone Description: Backbone Size:2870; Vector Backbone:pCRRNAcos; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29519933
Proper citation: RRID:Addgene_101821 Copy
Species: Synthetic
Genetic Insert: 3xERSE-I
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGL4.29; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32577017
Proper citation: RRID:Addgene_101789 Copy
Species: Synthetic
Genetic Insert: crRNAs targeting GFP and mCherry
Vector Backbone Description: Backbone Size:2870; Vector Backbone:pCRRNAcos; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29519933
Proper citation: RRID:Addgene_101804 Copy
Species: Synthetic
Genetic Insert: Lifeact
Vector Backbone Description: Backbone Size:8690; Vector Backbone:pMuLE Dest PuroR; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_101893 Copy
Species: Synthetic
Genetic Insert: PA-mCherry
Vector Backbone Description: Backbone Marker:SINF-EF-G (from Dr. Robert Hawley, George Washington University) was modified to make the lentiviral backbone.; Backbone Size:7500; Vector Backbone:pSin4-EF2-IRES-Pur; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30983487
Comments: There is a "NheI" restriction site which we artificially added between PAmCherry and SNCA-NE to facilitate cloning. i.e. 5'---PAmCherry-NheI-SNCA-NE----3'
This plasmid encodes a novel 18-amino-acid protein tag - "NE", which can be detected by a specific mouse monoclonal antibody in Western blotting, immunopreciptation, and immunocytochemistry. (Source of antibody: http://www.versitech.hku.hk/reagents/ne/)
Proper citation: RRID:Addgene_102364 Copy
Species: Synthetic
Genetic Insert: PA-mCherry
Vector Backbone Description: Backbone Marker:SINF-EF-G (from Dr. Robert Hawley, George Washington University) was modified to make the lentiviral backbone.; Backbone Size:7500; Vector Backbone:pSin4-EF2-IRES-Pur; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30983487
Comments: "KFERQ" is the consensus peptide sequence found in various native proteins for mediating degradation via Chaperone-Mediated Autophagy (CMA)
There is a "NheI" restriction site which we artificially added between PAmCherry and KFERQ-NE to facilitate cloning. i.e. 5'---PAmCherry-NheI-KFERQ-NE----3'
This plasmid encodes a novel 18-amino-acid protein tag - "NE", which can be detected by a specific mouse monoclonal antibody in Western blotting, immunopreciptation, and immunocytochemistry. (Source of antibody: http://www.versitech.hku.hk/reagents/ne/)
Proper citation: RRID:Addgene_102365 Copy
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