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Species: Homo sapiens
Genetic Insert: Tau E14
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21172610
Comments: WT htau construct (Addgene plasmid 46904) was used as a template to make mutations and contains human four-repeat tau lacking the N-terminal sequences (4R0N) and contains exons 1, 4 and 5, 7, and 9–13, intron 13, and exon 14.
Proper citation: RRID:Addgene_46907 Copy
Species: Homo sapiens
Genetic Insert: Tau AP P301L
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21172610
Comments: WT htau construct (Addgene plasmid 46904) was used as a template to make mutations and contains human four-repeat tau lacking the N-terminal sequences (4R0N) and contains exons 1, 4 and 5, 7, and 9–13, intron 13, and exon 14.
Proper citation: RRID:Addgene_46906 Copy
Species: Homo sapiens
Genetic Insert: Triple MBT domain from human L3MBTL1
Vector Backbone Description: Backbone Marker:GE Healthcare; Backbone Size:4984; Vector Backbone:pGEX-6P-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23583077
Comments: Original description of the plasmid in West et al. J Biol Chem. 2010 Nov 26;285(48):37725-32.
Proper citation: RRID:Addgene_46987 Copy
Species: Homo sapiens
Genetic Insert: AL-09
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:4674; Vector Backbone:pET12a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16751605
Proper citation: RRID:Addgene_47081 Copy
Genetic Insert: Cre
Vector Backbone Description: Backbone Marker:Addgene plasmid 31830; Backbone Size:3216; Vector Backbone:pT3TS; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21552255
Proper citation: RRID:Addgene_47117 Copy
Vector Backbone Description: Backbone Marker:Eric R Geertsma & Bert Poolman; Backbone Size:5824; Vector Backbone:pBADnLIC; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21410291
Proper citation: RRID:Addgene_47075 Copy
Vector Backbone Description: Backbone Marker:LM Guzman; Backbone Size:5779; Vector Backbone:pBAD24; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21410291
Proper citation: RRID:Addgene_47073 Copy
Vector Backbone Description: Backbone Marker:Eric R Geertsma & Bert Poolman; Backbone Size:5519; Vector Backbone:pRExLIC; Vector Types:Lactococcus lactis expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21410291
Proper citation: RRID:Addgene_47079 Copy
Species: E. coli
Genetic Insert: ltDHFR(DD)
Vector Backbone Description: Backbone Marker:Garry Nolan ; Backbone Size:6943; Vector Backbone:pBMN; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23991108
Comments: This N-terminal ltDHFR(DD) plasmid is the clone#22 in Table 1
Proper citation: RRID:Addgene_47076 Copy
Vector Backbone Description: Backbone Marker:LM Guzman; Backbone Size:6556; Vector Backbone:pBAD24; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21410291
Comments: Folding indicator GFP
Proper citation: RRID:Addgene_47070 Copy
Vector Backbone Description: Backbone Marker:LM Guzman; Backbone Size:5779; Vector Backbone:pBAD24; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21410291
Proper citation: RRID:Addgene_47071 Copy
Species: S. Pyogenes
Genetic Insert: dCas9-VP64
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23892895
Proper citation: RRID:Addgene_47107 Copy
Vector Backbone Description: Backbone Marker:LM Guzman; Backbone Size:5780; Vector Backbone:pBAD24; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21410291
Proper citation: RRID:Addgene_47068 Copy
Species: Homo sapiens
Genetic Insert: Forkhead Box P2
Vector Backbone Description: Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:21653829
Proper citation: RRID:Addgene_47053 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Codon-optimized sequences for MEV pathway expression in E. coli to produce limonene
Vector Backbone Description: Backbone Size:3500; Vector Backbone:pBbA5a; Vector Types:Bacterial Expression, Synthetic Biology, BglBrick; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23727191
Comments: The R364S mutation in the MK is not known to alter the function. The plasmid functions as described in the associated publication.
Proper citation: RRID:Addgene_47049 Copy
Genetic Insert: M2rtTA
Vector Backbone Description: Backbone Size:9846; Vector Backbone:ROSA26-1; Vector Types:Mouse Targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16400644
Proper citation: RRID:Addgene_47381 Copy
Species: Other
Genetic Insert: Cas9
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2; Vector Types:Mammalian Expression, CRISPR, xenopus and zebrafish expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24873830
Comments: Please see the Schier lab website http://labs.mcb.harvard.edu/schier/ for detailed protocols about target site selection, sgRNA production, stop codon cassette design, Cas9 protein purification, injection and downstream analysis.
This plasmid was used to prepare and inject Cas9 mRNA into zebrafish zygotes.
Proper citation: RRID:Addgene_47322 Copy
Species: Other
Genetic Insert: TRANSPORT INHIBITOR RESPONSE 1
Vector Backbone Description: Vector Backbone:pBabe-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23150568
Comments: Nishimura et al., 2009, Nature Methods
Please note that this version contains a nuclear export signal. A related construct without the NES can be found at: http://www.addgene.org/64945/
Proper citation: RRID:Addgene_47328 Copy
Species: Homo sapiens
Genetic Insert: Rab35
Vector Backbone Description: Backbone Marker:clontech; Backbone Size:4731; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23264734
Proper citation: RRID:Addgene_47424 Copy
Species: Caenorhabditis elegans
Genetic Insert: Glutamate-gated chloride channels
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2037; Vector Backbone:pcDNA3.1/V5-His TOPO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23720773
Proper citation: RRID:Addgene_47542 Copy
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