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Genetic Insert: TALE Nuclease, NG for 0.5 repeat
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:22222791
Proper citation: RRID:Addgene_32190 Copy
Genetic Insert: TALE Transcriptional Activator Backbone, NI for 0.5 repeat
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:22222791
Proper citation: RRID:Addgene_32187 Copy
Genetic Insert: TALE Transcriptional Activator Backbone, NN for 0.5 repeat
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:22222791
Proper citation: RRID:Addgene_32185 Copy
Genetic Insert: ccdB
Vector Backbone Description: Vector Backbone:pJFRC7; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21931740
Comments: Please see http://www.everyvector.com/sequences/show_public/13276 for additional plasmid map.
Proper citation: RRID:Addgene_32318 Copy
Vector Backbone Description: Backbone Marker:Mo Bi Tec; Vector Backbone:pET01; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:18930792
Proper citation: RRID:Addgene_32484 Copy
Vector Backbone Description: Backbone Size:7032; Vector Backbone:pLKO.1 puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21418658
Comments: The full plasmid sequence was assembled and may contain minor discrepancies, which should not affect the function of the plasmid.
Proper citation: RRID:Addgene_32684 Copy
Vector Backbone Description: Backbone Size:8453; Vector Backbone:pLKO.1 puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21418658
Proper citation: RRID:Addgene_32682 Copy
Vector Backbone Description: Backbone Size:7032; Vector Backbone:pLKO.1 puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21418658
Comments: The full plasmid sequence was digitally assembled and may contain minor discrepancies, which should not effect the function of the plasmid.
Proper citation: RRID:Addgene_32685 Copy
Vector Backbone Description: Backbone Size:4427; Vector Backbone:pACAGW-ChR2-Venus-AAV; Vector Types:AAV, aav; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21772812
Proper citation: RRID:Addgene_32671 Copy
Vector Backbone Description: Backbone Marker:Addgene Yeast Gateway collection (Lindquist / Gitler); Backbone Size:7574; Vector Backbone:pRS424-GPD-ccdB; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:29212664
Proper citation: RRID:Addgene_70124 Copy
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGL3-Promoter; Vector Types:Insect Expression, flySTARR-seq screening vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:23328393
Comments: This plasmid was made by replacing the sequence of pGL3-Promoter between BglII and FseI with a fragment containing the core promoter listed above, an mhc16 intron, an ORF (sgGFP, Qbiogene, Inc), a ccdB suicide gene flanked by homology arms (used for cloning the enhancer candidates during library generation), followed by the pGL3's SV40 late polyA-signal.
Proper citation: RRID:Addgene_71500 Copy
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGL3-Promoter; Vector Types:Insect Expression, flySTARR-seq screening vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25517091
Comments: This plasmid was made by replacing the sequence of pGL3-Promoter between BglII and FseI with a fragment containing the core promoter listed above, an mhc16 intron, an ORF (sgGFP, Qbiogene, Inc), a ccdB suicide gene flanked by homology arms (used for cloning the enhancer candidates during library generation), followed by the pGL3's SV40 late polyA-signal.
Proper citation: RRID:Addgene_71501 Copy
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGL3-Promoter; Vector Types:Insect Expression, flySTARR-seq screening vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25517091
Comments: This plasmid was made by replacing the sequence of pGL3-Promoter between BglII and FseI with a fragment containing the core promoter listed above, an mhc16 intron, an ORF (sgGFP, Qbiogene, Inc), a ccdB suicide gene flanked by homology arms (used for cloning the enhancer candidates during library generation), followed by the pGL3's SV40 late polyA-signal.
Proper citation: RRID:Addgene_71505 Copy
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGL3-Promoter; Vector Types:Insect Expression, flySTARR-seq validation vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:23328393
Comments: We replaced the SV40 promotor of the pGL3-promotor plasmid by DSCP and inserted a Gateway-cassette into the multiple-cloning-site (MCS; using KpnI and BglII), i.e. upstream of the DSCP, to allow Gateway cloning of candidate sequences
Proper citation: RRID:Addgene_71506 Copy
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGL4.10; Vector Types:Mammalian Expression, humanSTARR-seq screening vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:23328393
Comments: Note: The original human STARR-seq plasmid has been used in the Arnold et al. 2013 publication. It should be replaced for all screens in mammalian cells with the updated version reported by Muerdter et al. 2017 for much improved signal-to-noise, especially for screens with highly complex libraries (e.g. those covering an entire mammalian genome). The improved version is Addgene plasmid # 99296. For enhancer-activity measurements in mammalian cells, please also consider the possibility of a plasmid-induced innate immune response (see Muerdter et al., 2017).
The sequence between SacI and AfeI on pGL4 was replaced by the fragment containing the Super Core Promoter 1 (SCP1), a synthetic intron (pIRESpuro3, Clontech; cat. no. 631619) an ORF (sgGFP, Qbiogene, Inc), a ccdB suicide gene flanked by homology arms, and the pGL3's SV40 late polyA-signal.
Proper citation: RRID:Addgene_71509 Copy
Species: Other
Genetic Insert: LSL-Tomato
Vector Backbone Description: Vector Backbone:Rosa26; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:26018130
Proper citation: RRID:Addgene_66590 Copy
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5010; Vector Backbone:pGL3; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:26658467
Proper citation: RRID:Addgene_66937 Copy
Vector Backbone Description: Vector Backbone:pLTE EGFP; Vector Types:Cell-free expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25529348
Comments: attR1-ccdB-CamR-attR2-eGFP-8xHis-Stop
Proper citation: RRID:Addgene_67138 Copy
Vector Backbone Description: Vector Backbone:pLTE EGFP; Vector Types:Cell-free expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25529348
Comments: attR1-ccdB-CamR-attR2-mCheery-8xHis-Myc-Stop
Proper citation: RRID:Addgene_67142 Copy
Vector Backbone Description: Vector Backbone:pCellFree_G08; Vector Types:Cell-free expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25529348
Comments: sfGFP-8xHis
Proper citation: RRID:Addgene_67144 Copy
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