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Genetic Insert: Nuclear-localized Infrared Fluorescent Protein
Vector Backbone Description: Backbone Marker:OTTC/NIDA; Vector Backbone:pOTTC475; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28380331
Proper citation: RRID:Addgene_59132 Copy
Species: Homo sapiens
Genetic Insert: Oct4
Vector Backbone Description: Backbone Marker:Dowdy lab; Vector Backbone:T7-VEE-IRES-puro (Addgene plasmid 58970); Vector Types:Mammalian Expression, Venezuelan equine encephalitis (VEE) virus RNA replicon; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23910086
Comments: Summary of features:
1-7561: VEE RNA replicases (nsP1, nsP2, nsP3, nsP4 and 26S promoter, nsP1~4 are nonstructural VEE sequences which encode RNA replicases)
7592-8671: human Oct4
8678-8731: T2A peptide sequence
8738-10147: human Klf4
10154-10213: E2A peptide sequence
10223-11176: human Sox2
11195-11805: IRES sequence
11818-13140: human cMyc
13165-13776: IRES sequence
13777-14376: Puromycin resistance gene
14383-14510:VEE 3’UTR and poly A tail
14679-15539: Ampicillin resistance gene
16319-16336: T7 promoter
Proper citation: RRID:Addgene_58972 Copy
Vector Backbone Description: Backbone Marker:gift from I. Frolov; Backbone Size:10793; Vector Backbone:VEE backbone; Vector Types:Mammalian Expression, Venezuelan equine encephalitis (VEE) virus RNA replicon; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23910086
Proper citation: RRID:Addgene_58971 Copy
Species: Synthetic
Genetic Insert: AAVS1-TALEN-R
Vector Backbone Description: Backbone Marker:Feng Zhang Lab; Backbone Size:7961; Vector Backbone:pTALEN; Vector Types:Mammalian Expression, AAV, TALEN; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24931489
Proper citation: RRID:Addgene_59026 Copy
Species: Aequorea victoria
Genetic Insert: EGFP
Vector Backbone Description: Backbone Marker:Dowdy lab; Vector Backbone:Sp6-VEE-IRES-puro (Addgene plasmid 58971); Vector Types:Mammalian Expression, Venezuelan equine encephalitis (VEE) virus RNA replicon; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23910086
Comments: For author-annotated map, please see T7-VEE-GFP (Addgene plasmid 58977).
Proper citation: RRID:Addgene_58976 Copy
Species: Homo sapiens
Genetic Insert: CAMSAP2
Vector Backbone Description: Vector Backbone:pFasBac; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24706919
Proper citation: RRID:Addgene_59037 Copy
Species: Homo sapiens
Genetic Insert: CAMSAP2 CC+CKK domains
Vector Backbone Description: Vector Backbone:pFasBac; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24706919
Proper citation: RRID:Addgene_59039 Copy
Species: Mus musculus
Genetic Insert: Mouse IRF4
Vector Backbone Description: Vector Backbone:MIG; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25006123
Proper citation: RRID:Addgene_58987 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Backbone Size:4872; Vector Backbone:pUG35; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26184437
Proper citation: RRID:Addgene_58980 Copy
Species: Synthetic
Genetic Insert: mito-ZapCY1 high affinity Zn(II) sensor
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22850482
Comments: Genetically encoded, ratiometric, fluorescent biosensor. mito-ZapCY1 contains 4 tandem repeats of the first 29 aa of human cox subunit 8a (MSVLTPLLLRGLTGSARRLPVPRAKIHSL). HindIII restriction sites are at the 5' and 3' ends of the mitochondrial targeting sequence. Zn(II)-binding domain derived from the first 2 zinc fingers of Zap 1 from Saccharomyces cerevisiae. Contains truncated CFP and Citrine fluorescent proteins.
Proper citation: RRID:Addgene_58996 Copy
Species: Homo sapiens
Genetic Insert: Rb
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24876129
Comments: *CDK mutations (all changed to Ala): T5, S239, S249, T252, T356, T373, S608, S612, S780, S788, S795, S807, S811, T821, T826
Proper citation: RRID:Addgene_58914 Copy
Species: Homo sapiens
Genetic Insert: Rb
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24876129
Comments: *CDK mutations (all changed to Ala): T5, S239, S249, T252, T356, T373, S608, S612, S780, S788, S795, S807, S811, T821, T826
Proper citation: RRID:Addgene_58918 Copy
Species: Drosophila melanogaster
Genetic Insert: Patronin
Vector Backbone Description: Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24706919
Proper citation: RRID:Addgene_59053 Copy
Species: Stigeoclonium helveticum
Genetic Insert: Chronos-GFP
Vector Backbone Description: Backbone Marker:Addgene (Plasmid 11159); Backbone Size:5117; Vector Backbone:pCAGIG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24509633
Comments: The depositing lab sequenced this plasmid completely except for the CAG promoter.
Proper citation: RRID:Addgene_59056 Copy
Species: Chloromonas oogama
Genetic Insert: CoChR-GFP
Vector Backbone Description: Backbone Size:4368; Vector Backbone:AAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24509633
Comments: Plasmid is completely sequenced by the depositing lab except for part of the 3' ITR. Addgene QC NGS analysis identifies ambiguous bases following the 3' ITR. It is unknown what, if any, impact this may have on plasmid function.
Proper citation: RRID:Addgene_59070 Copy
Genetic Insert: mpeg1-mcherry
Vector Backbone Description: Backbone Size:3500; Vector Backbone:Tol2; Vector Types:; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_58935 Copy
Genetic Insert: inLuc
Vector Backbone Description: Vector Backbone:pHTC; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20195464
Comments: Addgene QC results show a small discrepancy within the intron when compared to the full sequence. This does not have any functional consequence.
Proper citation: RRID:Addgene_58954 Copy
Species: Gallus gallus
Genetic Insert: Focal Adhesion Kinase
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5002; Vector Backbone:pcDNA-5/FRT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23393139
Comments: Discrepancies between QC and full sequence are of no functional consequence
Proper citation: RRID:Addgene_59121 Copy
Species: Homo sapiens
Genetic Insert: p38 Kinase Translocation Reporter
Vector Backbone Description: Vector Backbone:pENTR; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24949979
Proper citation: RRID:Addgene_59149 Copy
Species: Homo sapiens
Genetic Insert: FOS
Vector Backbone Description: Vector Backbone:pLX304; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24954536
Proper citation: RRID:Addgene_59140 Copy
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