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Species: E. coli
Genetic Insert: Eco1 RT and recombineering ncRNA, priB G1069A, a1/a2 length: 12
Vector Backbone Description: Vector Backbone:pET-21(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34949838
Proper citation: RRID:Addgene_184962 Copy
Species: E. coli
Genetic Insert: Eco1 editing ncRNA and gRNA, FAA1 P233X, a1/a2 length: 27 v1
Vector Backbone Description: Vector Backbone:pZS165 (Addgene plasmid #114451); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34949838
Proper citation: RRID:Addgene_184982 Copy
Species: E. coli
Genetic Insert: Eco1 editing ncRNA and gRNA, FAA1 P233X, a1/a2 length: 12
Vector Backbone Description: Vector Backbone:pZS165 (Addgene plasmid #114451); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34949838
Proper citation: RRID:Addgene_184981 Copy
Species: E. coli
Genetic Insert: Eco1 RT and recombineering ncRNA, murF G1359A, a1/a2 length: 12
Vector Backbone Description: Vector Backbone:pET-21(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34949838
Proper citation: RRID:Addgene_184960 Copy
Species: E. coli
Genetic Insert: Eco1: RT and ncRNA(wt), a1/a2 length: 12, dead RT
Vector Backbone Description: Vector Backbone:pZS165 (Addgene plasmid #114451); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34949838
Proper citation: RRID:Addgene_184966 Copy
Species: E. coli
Genetic Insert: Integrating inducible cassette: Eco1 RT
Vector Backbone Description: Vector Backbone:pZS157 (Addgene plasmid #114454); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34949838
Proper citation: RRID:Addgene_184970 Copy
Species: E. coli
Genetic Insert: Eco1 editing ncRNA and gRNA, TRP2 E64X, a1/a2 length: 12
Vector Backbone Description: Vector Backbone:pZS165 (Addgene plasmid #114451); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34949838
Proper citation: RRID:Addgene_184979 Copy
Species: E. coli
Genetic Insert: LacI
Vector Backbone Description: Backbone Marker:System Biosciences; Backbone Size:6892; Vector Backbone:PB-EF1α-MCS-IRES-Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35058555
Proper citation: RRID:Addgene_179510 Copy
Species: E. coli
Genetic Insert: RNase III
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36493370
Proper citation: RRID:Addgene_189537 Copy
Species: E. coli
Genetic Insert: MG1655-C321 mutS+, λ-, Δ(ybhB-bioAB)::zeoR, ΔprfA, ΔserB
Vector Backbone Description: Vector Backbone:N/A; Vector Types:; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:36280721
Comments: MG1655-C321 -- All 321 UAG codons changed to UAA.
mutS+ -- Restores normal spontaneous mutation frequency.
(λ-) – Deleted Lambda Phage operon to allow growth at 37C.
Δ(ybhB-bioAB)::zeoR – Deletion of biotin biosynthetic operon using Zeocin cassette; strain is d-biotin auxotroph.
ΔprfA – Deletion of polypeptide release factor 1 (RF1) to prevent translation termination at UAG codons.
ΔserB – Deletion of phosphoserine phosphatase to provide intracellular source of phosphoserine; strain is Serine auxotroph.
The genome sequence of a similar strain (C321.deltaA) was deposited at NCBI (accession # CP006698.1)
Proper citation: RRID:Addgene_192872 Copy
Species: E. coli
Genetic Insert: lacZ
Vector Backbone Description: Vector Backbone:pXD69m2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:36494336
Proper citation: RRID:Addgene_191620 Copy
Species: E. coli
Genetic Insert: lacZ
Vector Backbone Description: Vector Backbone:pXD69m2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:36494336
Proper citation: RRID:Addgene_191621 Copy
Species: E. coli
Genetic Insert: lacZ
Vector Backbone Description: Vector Backbone:pXD69m2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:36494336
Proper citation: RRID:Addgene_191627 Copy
Species: E. coli
Genetic Insert: lacZ
Vector Backbone Description: Vector Backbone:pXD69m2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:36494336
Proper citation: RRID:Addgene_191625 Copy
Species: E. coli
Genetic Insert: lacZ
Vector Backbone Description: Vector Backbone:pXD69m2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:36494336
Proper citation: RRID:Addgene_191629 Copy
Species: E. coli
Genetic Insert: human RNaseH1 wild type
Vector Backbone Description: Vector Backbone:pEGFP-N2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36544021
Proper citation: RRID:Addgene_196702 Copy
Species: E. coli
Genetic Insert: wt E. coli SSB
Vector Backbone Description: Backbone Marker:EMB/Novagen; Backbone Size:5443; Vector Backbone:pET21a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24021816
Proper citation: RRID:Addgene_197260 Copy
Species: E. coli
Genetic Insert: bacterial RNaseHI
Vector Backbone Description: Vector Backbone:pLVX; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36544021
Proper citation: RRID:Addgene_196701 Copy
Species: E. coli
Genetic Insert: MscL(G22S)-c-term-cpGFP-ERexp
Vector Backbone Description: Backbone Size:6100; Vector Backbone:Puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36608338
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.10.04.510821v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_195346 Copy
Species: E. coli
Genetic Insert: acrAB
Vector Backbone Description: Backbone Size:3658; Vector Backbone:pBbA5k; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30310093
Proper citation: RRID:Addgene_195516 Copy
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